US4698312A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Stabilizing blood cells with aromatic polyaldehyde for use in hematology controls and calibrators
Assignee: IL Holding Spa
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Filing: 1986-07-28
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Granted: 1987-10-06
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Expires: 2006-07-28
Abstract
Abstract Blood cells such as erythrocytes of animal origin are treated with a solution containing water, an organic cosolvent (such as dimethylsulfoxide, alone or admixed with a polyol), an aromatic polyaldehyde (such as phthaldialdehyde) and optionally salts and buffers. In an illustrative case, the treating solution is hypertonic and shrinks goat erythrocytes to the size of human platelets.
Inventors
- 👤 Show-Chu Wong
- 👤 Harbans S. Deol
- 👤 Debra L. Harz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 11:02
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N2496/00; G01N2496/05; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/106664
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
61 words
A method for preparing a stabilized cell component of a hematology control or calibrator by reacting blood cells with an aqueous aldehyde solution, characterized by the aqueous aldehyde solution comprising: (a) water, (b) an aromatic polyaldehyde having carbonyls attached to aromatic ring carbons, and (c) an organic co-solvent in an amount miscible with water and sufficient to solubilize the aromatic polyaldehyde.
Claim 2
Independent Claim
61 words
A method for preparing a stabilized cell component of a hematology control or calibrator by reacting blood cells with an aqueous aldehyde solution, characterized by the aqueous aldehyde solution comprising: (a) water, (b) an aromatic polyaldehyde having carbonyls attached to aromatic ring carbons, and (c) an organic co-solvent in an amount miscible with water and sufficient to solubilize the aromatic polyaldehyde.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the aromatic polyaldehyde is a dialdehyde with two carbonyls attached to adjacent aromatic ring carbons.
Claim 4
Dependent Claim
11 words
The method of claim 2 wherein the aromatic polyaldehyde is phthaldialdehyde.
Claim 5
Dependent Claim
26 words
The method of claim 3 wherein the blood cells reacted with the aromatic polyaldehyde are goat erythrocytes and the fixed cell component is a platelet analog.
Claim 6
Dependent Claim
25 words
The method of claim 4 wherein the aqueous aldehyde solution is sufficiently hypertonic to shrink the goat erythrocytes to substantially the size of human platelets.
Claim 7
Dependent Claim
26 words
The method of claim 1 wherein the blood cells reacted with the aromatic polyaldehyde are goat erythrocytes and the fixed cell component is a platelet analog.
Claim 8
Dependent Claim
25 words
The method of claim 6 wherein the aqueous aldehyde solution is sufficiently hypertonic to shrink the goat erythrocytes to substantially the size of human platelets.
Claim 9
Dependent Claim
25 words
The method of claim 1 wherein the aqueous aldehyde solution is sufficiently hypertonic to shrink the blood cells to substantially the size of human platelets.
Claim 10
Dependent Claim
27 words
The method of claim 1 wherein the aqueous aldehyde solution is sufficiently hypotonic to swell the blood cells to substantially the size of human white blood cells.
Claim 11
Dependent Claim
12 words
The method of claim 9 wherein the blood cells are avian erythrocytes.
Claim 12
Dependent Claim
12 words
The method of claim 9 wherein the blood cells are human erythrocytes.
Claim 13
Dependent Claim
11 words
The method of claim 1 wherein the organic cosolvent is dimethylsulfoxide.
Claim 14
Dependent Claim
20 words
The method of claim 1 wherein the co-solvent is a mixture of dimethylsulfoxide with a watersoluble polyol of 2-8 carbons.
Claim 15
Dependent Claim
11 words
The method of claim 13 wherein the watersoluble polyol is glycerol.
Claim 16
Dependent Claim
26 words
A hemotology control component comprising an aqueous suspension of blood cells which have been stabilized with an aromatic polyaldehyde having carbonyls attached to aromatic ring carbons.
Claim 17
Dependent Claim
26 words
A hemotology control component comprising an aqueous suspension of blood cells which have been stabilized with an aromatic polyaldehyde having carbonyls attached to aromatic ring carbons.
Claim 18
Dependent Claim
23 words
The hematology control component of claim 15 wherein the aromatic polyaldehyde is a dialdehyde with two carbonyls attached to adjacent aromatic ring carbons.
Claim 19
Dependent Claim
13 words
The hematology control component of claim 16 wherein the aromatic polyaldehyde is phthaldialdehyde.
Claim 20
Dependent Claim
22 words
The hematology control component of claim 17 wherein the blood cells are goat erythrocytes shrunken to substantially the size of human platelets.
Claim 21
Dependent Claim
24 words
The hematology control component of claim 17 wherein the blood cells are avian erythrocytes swelled to substantially the size of human white blood cells.
Claim 22
Dependent Claim
24 words
The hematology control component of claim 15 wherein the blood cells are avian erythrocytes swelled to substantially the size of human white blood cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-10-05 | 1991-04-06 | 1991-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-10-05 | 1995-04-06 | 1995-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-10-05 | 1999-04-06 | 1999-10-06 | $7700.00 | Window Closed / Lapsed |