US4579824A
Expired / Lapsed
US
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Hematology control
Assignee: Individual
•
Filing: 1983-05-18
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Granted: 1986-04-01
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Expires: 2003-05-18
Abstract
Abstract A hematology control is disclosed which is useful for clinical hematology procedures and, in particular, mean corpuscular volume (MCV) determinations. The hematology control comprises an aqueous suspension of red blood cells in which varying levels of MCV are provided by applying to the red cell component, after light treatment with aldehyde, an osmotic pressure that is directly proportional to the desired red blood cell size.
Inventors
- 👤 Allan L. Louderback
- 👤 Paul Szatkowski
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 09:33
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/96; G01N2496/00; G01N2496/05; Y; Y10; Y10T; Y10T436/00; Y10T436/10; Y10T436/101666; Y10T436/107497
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
145 words
A hematology control for mean corpuscular volume determinations comprising an aqueous suspension of mammalian red blood cells in which said red blood cells have been treated to provide a predetermined mean corpuscular volume by contacting with an aqueous aldehyde solution in 0.9% saline in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18° to about 28° C., whereby said red blood cells retain their plasticity for up to about 5 days and then washing said red cells while still retaining said plasticity with Alsever's Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to provide an osmotic pressure on the cells which is directly proportional to the cell size required for the predetermined mean corpuscular volume.
Claim 2
Dependent Claim
145 words
A hematology control for mean corpuscular volume determinations comprising an aqueous suspension of mammalian red blood cells in which said red blood cells have been treated to provide a predetermined mean corpuscular volume by contacting with an aqueous aldehyde solution in 0.9% saline in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18° to about 28° C., whereby said red blood cells retain their plasticity for up to about 5 days and then washing said red cells while still retaining said plasticity with Alsever's Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to provide an osmotic pressure on the cells which is directly proportional to the cell size required for the predetermined mean corpuscular volume.
Claim 3
Dependent Claim
33 words
The hematology control of claim 1 in which the predetermined mean corpuscular volume ranges from about 75 to about 95μ 3 and the osmotic pressure ranges from about 115 to about 275 milliosmols.
Claim 4
Dependent Claim
18 words
The hematology control of claim 1 in which the mammalian red blood cells are human red blood cells.
Claim 5
Dependent Claim
31 words
The hematology control of claim 3 including simulated human white blood cells comprising avian red blood cells which have been stringently fixed with dilute aldehyde solution whereby said cells become hard.
Claim 6
Dependent Claim
23 words
The hematology control of claim 1 in which the red blood cells are suspended in 0.9% saline solution during said contacting with aldehyde.
Claim 7
Dependent Claim
12 words
The hematology control of claim 1 in which the aldehyde is formaldehyde.
Claim 8
Dependent Claim
50 words
The hematology control of claim 4 in which the concentration of the human red blood cells ranges from about 3×10 6 to about 5×10 6 /mm 3 and in which the concentration of the avian red blood cells ranges from about 5×10 3 to about 15×10 3 /mm 3 .
Claim 9
Independent Claim
157 words
A method of making a hematology control having a predetermined mean corpuscular volume comprising treating mammalian red blood cells with an aqueous solution of aldehyde in 0.9% saline solution in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18° to about 28° C., whereby said red cells retain their plasticity for up to about 5 days, adjusting the mean corpuscular volume to any desired level within a range of from about 75 to about 95μ 3 by thoroughly washing the red blood cells while still retaining said plasticity in Alsever's Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to thereby provide an osmotic pressure on the red blood cells of from about 115 to about 275 milliosmols, and thereafter placing the red blood cells in aqueous suspension.
Claim 10
Independent Claim
157 words
A method of making a hematology control having a predetermined mean corpuscular volume comprising treating mammalian red blood cells with an aqueous solution of aldehyde in 0.9% saline solution in proportions equivalent to about 40 ml of 37% formaldehyde and about 500 ml saline, for about 2 hours at temperature of from about 18° to about 28° C., whereby said red cells retain their plasticity for up to about 5 days, adjusting the mean corpuscular volume to any desired level within a range of from about 75 to about 95μ 3 by thoroughly washing the red blood cells while still retaining said plasticity in Alsever's Solution having a pH of from about 6.8 to about 7.4 and modified to contain from about 0.01% to about 0.21% sodium chloride to thereby provide an osmotic pressure on the red blood cells of from about 115 to about 275 milliosmols, and thereafter placing the red blood cells in aqueous suspension.
Claim 11
Dependent Claim
17 words
The method of claim 8 in which the mammalian red blood cells are human red blood cells.
Claim 12
Dependent Claim
11 words
The method of claim 8 in which the aldehyde is formaldehyde.
Claim 13
Dependent Claim
12 words
The method of claim 8 in which the pH is about 7.2.
Claim 14
Dependent Claim
27 words
The method of claim 8 in which the modified Alsever's Solution contains a sugar component consisting of sucrose having a concentration of about 10 gm per liter.
Claim 15
Dependent Claim
27 words
The hematology control of claim 1 including platelets which have been treated with aldehyde and modified Alsever's Solution in the same manner as the red blood cells.
Claim 16
Dependent Claim
28 words
The hematology control of claim 3 including human platelets which have been treated with aldehyde and modified Alsever's Solution in the same manner as the red blood cells.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-31 | 1989-09-30 | 1990-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-31 | 1993-09-30 | 1994-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-31 | 1997-09-30 | 1998-04-01 | $7700.00 | Window Closed / Lapsed |