US4379847A
Expired / Lapsed
US
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Suspending medium for immunologic reactions
Assignee: American Hospital Supply Corp
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Filing: 1981-01-14
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Granted: 1983-04-12
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Expires: 2001-01-14
Abstract
Abstract A low ionic strength suspending medium for immunologic reactions which includes a salt solution, a buffer, gelatin, albumin, and an organic solute to control osmolality.
Inventors
- 👤 Mitchell J. Fruitstone
- 👤 Michele M. Tilly
- 👤 Betty G. Pixton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 15:32
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5306; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S436/00; Y10S436/826; Y10T; Y10T436/00; Y10T436/10; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
114 words
A suspending medium for an immunologic reaction comprising gelatin, having a Bloom rating from about 75 to about 300 at a concentration such that the Bloom rating times concentration in weight percent is from about 40 to about 150; albumin, at a concentration of from about 3.0 wt. % to about 7.0 wt. %; sufficient organic solute to provide an osmolality of from about 150 mOsm/kg. H 2 O to about 450 mOsm/kg. H 2 O; sufficient salt of an alkali or alkaline earth metal to provide an ionic strength equivalent to about a 0.01 molar to about a 0.10 molar solution of sodium chloride; and a pH from about 6.0 to about 8.0.
Claim 2
Dependent Claim
114 words
A suspending medium for an immunologic reaction comprising gelatin, having a Bloom rating from about 75 to about 300 at a concentration such that the Bloom rating times concentration in weight percent is from about 40 to about 150; albumin, at a concentration of from about 3.0 wt. % to about 7.0 wt. %; sufficient organic solute to provide an osmolality of from about 150 mOsm/kg. H 2 O to about 450 mOsm/kg. H 2 O; sufficient salt of an alkali or alkaline earth metal to provide an ionic strength equivalent to about a 0.01 molar to about a 0.10 molar solution of sodium chloride; and a pH from about 6.0 to about 8.0.
Claim 3
Dependent Claim
20 words
The suspending medium of claim 1 wherein the gelatin Bloom rating times concentration is from about 40 to about 130.
Claim 4
Dependent Claim
21 words
The suspension medium of claim 2 wherein the albumin concentration is from about 4.0 wt. % to about 6.0 wt. %.
Claim 5
Dependent Claim
15 words
The suspending medium of claim 3 wherein the albumin is substantially salt-free bovine serum albumin.
Claim 6
Dependent Claim
35 words
The suspending medium of claim 1 wherein the osmolality is from about 250 mOsm/kg. H 2 O to about 400 mOsm/kg. H 2 O, and the salt is sodium chloride, potassium chloride, or sodium azide.
Claim 7
Dependent Claim
48 words
The suspending medium of claim 3 wherein the osmolality is from about 250 mOsm/kg. H 2 O to about 400 mOsm/kg. H 2 O, the gelatin Bloom rating times concentration is from about 45 to about 90, and the salt is sodium chloride, potassium chloride, or sodium azide.
Claim 8
Dependent Claim
35 words
The suspending medium of claim 6 wherein the organic solute is an amino acid, a sugar, or a water soluble alcohol, and the gelatin has a Bloom rating of from about 100 to about 275.
Claim 9
Dependent Claim
15 words
The suspending medium of claim 6 wherein the organic solute is glycine, sucrose, or glucose.
Claim 10
Dependent Claim
12 words
The suspending medium of claim 6 wherein the organic solute is glycine.
Claim 11
Dependent Claim
16 words
The suspending medium of claim 1 wherein the pH is from about 6.4 to about 7.4.
Claim 12
Dependent Claim
16 words
The suspending medium of claim 3 wherein the pH is from about 6.4 to about 7.4.
Claim 13
Dependent Claim
16 words
The suspending medium of claim 8 wherein the pH is from about 6.4 to about 7.4.
Claim 14
Dependent Claim
12 words
The suspending medium of claim 1 wherein the salt is sodium azide.
Claim 15
Dependent Claim
12 words
The suspending medium of claim 3 wherein the salt is sodium azide.
Claim 16
Dependent Claim
12 words
The suspending medium of claim 8 wherein the salt is sodium azide.
Claim 17
Dependent Claim
15 words
13. The suspending medium of claim 1, further comprising a pH-controlling amount of a buffer.
Claim 18
Dependent Claim
13 words
The suspending medium of claim 16 wherein the buffer is a phosphate buffer.
Claim 19
Dependent Claim
15 words
The suspending medium of claim 3, further comprising a pH-controlling amount of a phosphate buffer.
Claim 20
Dependent Claim
15 words
The suspending medium of claim 8, further comprising a pH-controlling amount of a phosphate buffer.
Claim 21
Dependent Claim
17 words
The suspending medium of claim 1, further comprising a preserving amount of a bacteriostat or an antibiotic.
Claim 22
Dependent Claim
23 words
The suspending medium of claim 20 wherein the antibiotic is neomycin or chloramphenicol, or the bacteriostat is thimerosal, phenylmercuric acetate, or sodium azide.
Claim 23
Dependent Claim
13 words
The suspending medium of claim 3, further comprising a preserving amount of thimerosal.
Claim 24
Dependent Claim
13 words
The suspending medium of claim 8, further comprising a preserving amount of theimerosal.
Claim 25
Dependent Claim
13 words
The suspending medium of claim 19, further comprising a preserving amount of thimerosal.
Claim 26
Dependent Claim
33 words
The suspending medium of claim 1, 3, 4, 8, 9, 12, 15, 19 or 23, wherein the ionic strength is equivalent to about a 0.02 molar to about 0.05 molar sodium chloride solution.
Claim 27
Dependent Claim
14 words
The suspending medium of claim 25 wherein the immunologic reaction is an immunohematologic reaction.
Claim 28
Dependent Claim
33 words
The suspending medium of claim 24 wherein the gelatin has a Bloom rating of about 100 and is employed at a concentration of from about 0.7 wt. % to about 1.3 wt. %.
Claim 29
Dependent Claim
33 words
The suspending medium of claim 24 wherein the gelatin has a Bloom rating of about 260 and is employed at a concentration of from about 0.25 wt. % to about 0.5 wt. %.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-04-11 | 1986-10-11 | 1987-04-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-04-11 | 1990-10-11 | 1991-04-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-04-11 | 1994-10-11 | 1995-04-12 | $7700.00 | Window Closed / Lapsed |