US4259207A
Expired / Lapsed
US
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Suspending medium for immunologic reactions
Assignee: Baxter Healthcare Corp
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Filing: 1979-09-19
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Granted: 1981-03-31
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Expires: 1999-09-19
Abstract
Abstract A low ionic strength suspending medium for immunologic reactions which includes a salt solution, a buffer, gelatin, albumin, and an organic solute to control osmolality.
Inventors
- 👤 Mitchell J. Fruitstone
- 👤 Michele M. Tilly
- 👤 Betty G. Pixton
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 17:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5306; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S436/00; Y10S436/826; Y10T; Y10T436/00; Y10T436/10; Y10T436/107497; Y10T436/108331
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
98 words
A suspending medium for an immunologic reaction, comprising gelatin, at a concentration of from about 0.5 wt. % to about 1.5 wt. %; albumin, at a concentration of from about 4.0 wt. % to about 6.0 wt. %; sufficient organic solute to provide an osmolality of from about 150 mOsm/kg. H 2 O to about 450 mOsm/kg. H 2 O; sufficient sodium chloride, potassium chloride, or a mixture thereof to provide an ionic strength equivalent to about a 0.01 molar to about a 0.10 molar solution of sodium chloride; and a pH from about 6.0 to about 8.0.
Claim 2
Dependent Claim
98 words
A suspending medium for an immunologic reaction, comprising gelatin, at a concentration of from about 0.5 wt. % to about 1.5 wt. %; albumin, at a concentration of from about 4.0 wt. % to about 6.0 wt. %; sufficient organic solute to provide an osmolality of from about 150 mOsm/kg. H 2 O to about 450 mOsm/kg. H 2 O; sufficient sodium chloride, potassium chloride, or a mixture thereof to provide an ionic strength equivalent to about a 0.01 molar to about a 0.10 molar solution of sodium chloride; and a pH from about 6.0 to about 8.0.
Claim 3
Dependent Claim
21 words
The suspending medium of claim 1 wherein the gelatin concentration is from about 0.7 wt. % to about 1.3 wt. %.
Claim 4
Dependent Claim
34 words
The suspending medium of claim 1 wherein the gelatin concentration is from about 0.7 wt. % to about 1.3 wt. % and the albumin concentration is from about 4.0 wt.% to about 6.0 wt.%.
Claim 5
Dependent Claim
15 words
The suspending medium of claim 3 wherein the albumin is substantially salt-free bovine serum albumin.
Claim 6
Dependent Claim
24 words
The suspending medium of claim 1 wherein the osmolality is from about 250 mOsm/kg. H 2 O to about 400 mOsm/kg. H 2 O.
Claim 7
Dependent Claim
24 words
The suspending medium of claim 3 wherein the osmolality is from about 250 mOsm/kg. H 2 O to about 400 mOsm/kg. H 2 O.
Claim 8
Dependent Claim
21 words
The suspending medium of claim 6 wherein the organic solute is an amino acid, a sugar, or a water soluble alcohol.
Claim 9
Dependent Claim
15 words
The suspending medium of claim 6 wherein the organic solute is glycine, sucrose, or glucose.
Claim 10
Dependent Claim
12 words
The suspending medium of claim 6 wherein the organic solute is glycine.
Claim 11
Dependent Claim
17 words
The suspending medium of claim 1 wherein the pH is from about 6.4 to about 7.4 .
Claim 12
Dependent Claim
16 words
The suspending medium of claim 3 wherein the pH is from about 6.4 to about 7.4.
Claim 13
Dependent Claim
16 words
The suspending medium of claim 8 wherein the pH is from about 6.4 to about 7.4.
Claim 14
Dependent Claim
14 words
The suspending medium of claim 1, further comprising a pH-controlling amount of a buffer.
Claim 15
Dependent Claim
13 words
The suspending medium of claim 13 wherein the buffer is a phosphate buffer.
Claim 16
Dependent Claim
15 words
The suspending medium of claim 3, further comprising a pH-controlling amount of a phosphate buffer.
Claim 17
Dependent Claim
15 words
The suspending medium of claim 8, further comprising a pH-controlling amount of a phosphate buffer.
Claim 18
Dependent Claim
17 words
The suspending medium of claim 1, further comprising a preserving amount of a bacteriostat or an antibiotic.
Claim 19
Dependent Claim
23 words
The suspending medium of claim 17, wherein the antibiotic is neomycin or chloramphenicol, or the bacteriostat is thimerosal, phenylmercuric acetate, or sodium azide.
Claim 20
Dependent Claim
13 words
The suspending medium of claim 3, further comprising a preserving amount of thimerosal.
Claim 21
Dependent Claim
13 words
The suspending medium of claim 8, further comprising a preserving amount of thimerosal.
Claim 22
Dependent Claim
13 words
The suspending medium of claim 16, further comprising a preserving amount of thimerosal.
Claim 23
Dependent Claim
32 words
The suspending medium of claim 1, 3, 4, 8, 9, 12, 16, or 20, wherein the ionic strength is equivalent to about a 0.02 molar to about 0.05 molar sodium chloride solution.
Claim 24
Dependent Claim
14 words
The suspending medium of claim 22 wherein the immunologic reaction is an immunohematologic reaction.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-03-30 | 1984-09-29 | 1985-03-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-03-30 | 1988-09-29 | 1989-03-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-03-30 | 1992-09-29 | 1993-03-31 | $7700.00 | Window Closed / Lapsed |