US4777128A
Expired / Lapsed
US
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Fluorescence immunoassay involving energy transfer between two fluorophores
Assignee: Ethigen Corp
•
Filing: 1986-05-27
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Granted: 1988-10-11
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Expires: 2006-05-27
Abstract
Abstract In a fluorescence immunoassay system, first and second fluorophores are covalently bound to one of a member of a specific binding pair of liquid and receptor, the first of said fluorophores being capable of absorbing light at a first wavelength to produce light emission at a second wavelength, which second wavelength can be absorbed by the second fluorophore.
Inventors
- 👤 Arnold S. Lippa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 01:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/576; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 46
Claim 1
Independent Claim
68 words
A member of a specific binding pair of ligand and receptor, said member being covalently bound to first and second fluorophores, the first of said fluorophores being capable of absorbing light energy at a first wavelength to produce light energy emission at a second wavelength, which second wavelength can be absorbed by the second fluorophore, wherein specific binding of ligand and receptor affects energy transfer between said fluorophores.
Claim 2
Dependent Claim
68 words
A member of a specific binding pair of ligand and receptor, said member being covalently bound to first and second fluorophores, the first of said fluorophores being capable of absorbing light energy at a first wavelength to produce light energy emission at a second wavelength, which second wavelength can be absorbed by the second fluorophore, wherein specific binding of ligand and receptor affects energy transfer between said fluorophores.
Claim 3
Dependent Claim
24 words
The member of claim 1 wherein specific binding of the pair inhibits absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 4
Dependent Claim
24 words
The member of claim 1 wherein specific binding of the pair enhances absorption by the second fluorophore of light, emitted by the first fluorophore.
Claim 5
Dependent Claim
18 words
The member of claim 1 wherein said member is receptor and said receptor is antibody to said ligand.
Claim 6
Dependent Claim
17 words
The member of claim 4 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 7
Dependent Claim
18 words
The member of claim 4 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, Thyroxin or Digoxin.
Claim 8
Dependent Claim
25 words
The member of claim 1 wherein the second fluorsphore produces an emission of light at a third wavelength upon absorbing light of the second wavelength.
Claim 9
Dependent Claim
18 words
The member of claim 7 wherein said member is receptor and said receptor is antibody to said ligand.
Claim 10
Dependent Claim
17 words
The member of claim 8 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 11
Dependent Claim
18 words
The member of claim 8 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, Thyroxin or Digoxin.
Claim 12
Dependent Claim
24 words
The member of claim 7, wherein specific binding of the pair inhibits absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 13
Dependent Claim
23 words
The member of claim 11 wherein said member is receptor and said receptor is antibody to said ligand and said ligand is Digoxin.
Claim 14
Dependent Claim
18 words
The member of claim 11 wherein said member is receptor and said receptor is antibody to said ligand.
Claim 15
Dependent Claim
10 words
The member of claim 13 wherein said ligand is Digoxin.
Claim 16
Dependent Claim
17 words
The member of claim 11 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 17
Dependent Claim
23 words
The member of claim 15 wherein said member is receptor and said receptor is antibody to said ligand and said ligand is Digoxin.
Claim 18
Dependent Claim
24 words
The member of claim 7 wherein specific binding of the pair enhances absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 19
Dependent Claim
30 words
The member of claim 17 wherein said member is receptor and said receptor is antibody to said ligand and said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, or Thyroxin.
Claim 20
Dependent Claim
18 words
The member of claim 17 wherein said member is receptor and said receptor is antibody to said ligand.
Claim 21
Dependent Claim
17 words
The member of claim 19 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, or Thyroxin.
Claim 22
Dependent Claim
17 words
The member of claim 17 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 23
Dependent Claim
30 words
The member of claim 21 wherein said member is receptor and said receptor is antibody to said ligand and said ligand is Hepatitis B Surface Antigen, Imunoglobulin G, or Thyroxin.
Claim 24
Independent Claim
132 words
A method for determining the presence or amount of a first member of a specific binding pair of ligand and receptor in a fluid sample, the method comprising: (a) forming a reaction mixture of contacting said sample with a second member of said pair, said second member being covalently bound to first and second fluorophores, the first of said fluorophores being capable of absorbing light at a first wavelength to produce light emission at a second wavelength, which second wavelength can be absorbed by the second fluorophore, wherein specific binding of ligand and receptor affects energy transfer between said fluorophores; (b) irradiating the reaction mixture with light of the first wavelength; and (c) measuring the amount of fluorescence from one of said fluorophores in the reaction mixture as compared to a standard.
Claim 25
Independent Claim
132 words
A method for determining the presence or amount of a first member of a specific binding pair of ligand and receptor in a fluid sample, the method comprising: (a) forming a reaction mixture of contacting said sample with a second member of said pair, said second member being covalently bound to first and second fluorophores, the first of said fluorophores being capable of absorbing light at a first wavelength to produce light emission at a second wavelength, which second wavelength can be absorbed by the second fluorophore, wherein specific binding of ligand and receptor affects energy transfer between said fluorophores; (b) irradiating the reaction mixture with light of the first wavelength; and (c) measuring the amount of fluorescence from one of said fluorophores in the reaction mixture as compared to a standard.
Claim 26
Dependent Claim
24 words
The method of claim 23 wherein specific binding of the pair inhibits absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 27
Dependent Claim
24 words
The method of claim 23 wherein specific binding of the pair enhances absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 28
Dependent Claim
19 words
The method of claim 23 wherein said second member is receptor and said receptor is antibody to said ligand.
Claim 29
Dependent Claim
17 words
The method of claim 26 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 30
Dependent Claim
18 words
The method of claim 26 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, Thyroxin or Digoxin.
Claim 31
Dependent Claim
25 words
The method of claim 23 wherein the second fluorophore produces an emission of light at a third wavelength upon absorbing light of the second wavelength.
Claim 32
Dependent Claim
19 words
The method of claim 29 wherein said second member is receptor and said receptor is antibody to said ligand.
Claim 33
Dependent Claim
17 words
The method of claim 30 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 34
Dependent Claim
18 words
The method of claim 30 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, Thyroxin or Digoxin.
Claim 35
Dependent Claim
24 words
The method of claim 29 wherein specific binding of the pair inhibits absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 36
Dependent Claim
10 words
The method of claim 33 wherein said ligand is Digoxin.
Claim 37
Dependent Claim
19 words
The method of claim 33 wherein said second member is receptor and said receptor is antibody to said ligand.
Claim 38
Dependent Claim
10 words
The method of claim 35 wherein said ligand is Digoxin.
Claim 39
Dependent Claim
17 words
The method of claim 33 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 40
Dependent Claim
23 words
The method of claim 37 wherein said member is receptor and said receptor is antibody to said ligand and said ligand is Digoxin.
Claim 41
Dependent Claim
24 words
The method of claim 29 wherein specific binding of the pair enhances absorption by the second fluorophore of light emitted by the first fluorophore.
Claim 42
Dependent Claim
17 words
The method of claim 39 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, or Thyroxin.
Claim 43
Dependent Claim
19 words
The method of claim 39 wherein said second member is receptor and said receptor is antibody to said ligand.
Claim 44
Dependent Claim
17 words
The method of claim 41 wherein said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, or Thyroxin.
Claim 45
Dependent Claim
17 words
The method of claim 39 wherein the first fluorophore is fluorescein and the second fluorophore is eosin.
Claim 46
Dependent Claim
30 words
The method of claim 43 wherein said member is receptor and said receptor is antibody to said ligand and said ligand is Hepatitis B Surface Antigen, Immunoglobulin G, of Thyroxin.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-10-11 | 1992-04-11 | 1992-10-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-10-11 | 1996-04-11 | 1996-10-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-10-11 | 2000-04-11 | 2000-10-11 | $7700.00 | Window Closed / Lapsed |