US4542104A
Expired / Lapsed
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Phycobiliprotein fluorescent conjugates
Assignee: Leland Stanford Junior University
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Filing: 1983-04-06
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Granted: 1985-09-17
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Expires: 2003-04-06
Abstract
Abstract Fluorescent conjugates are employed providing combinations of a fluorescent sensitizer and a fluorescent phycobiliprotein. The conjugates find use in applications where large Stokes shifts, high absorption coefficients and high fluorescence quantum yields are desired. Particularly, combinations of phycobiliproteins are employed where the wavelength of excitation may be 50 nm or more different from the emission wavelength.
Inventors
- 👤 Lubert Stryer
- 👤 Alexander N. Glazer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 01:58
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/542; G01N33/531; G01N33/532; G01N33/533; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
55 words
A conjugate comprising an energy donor as a first component and a fluorescer as a second component joined together at an energy exchanging distance, wherein one of said first and second components is a phycobiliprotein and the two components have a spectral overlap integral of at least about 10 -16 cm 3 M -1 .
Claim 2
Dependent Claim
55 words
A conjugate comprising an energy donor as a first component and a fluorescer as a second component joined together at an energy exchanging distance, wherein one of said first and second components is a phycobiliprotein and the two components have a spectral overlap integral of at least about 10 -16 cm 3 M -1 .
Claim 3
Dependent Claim
14 words
A conjugate according to claim 1, wherein said first and second components are phycobiliproteins.
Claim 4
Dependent Claim
30 words
A conjugate according to claim 2, wherein said phycobiliproteins are covalently joined by a linking group wherein said first and second components are separated by not more than 10 nm.
Claim 5
Dependent Claim
17 words
A conjugate according to claim 3, wherein a portion of said linking group is a disulfide linkage.
Claim 6
Dependent Claim
22 words
A conjugate according to claim 1, wherein said fluorescer fluoresces at a wavelength in the range of about 550 to 800 nm.
Claim 7
Dependent Claim
15 words
A conjugate according to claim 1, wherein said first and second components are covalently joined.
Claim 8
Dependent Claim
102 words
A conjugate comprising two phycobiliproteins joined within energy exchanging distance, wherein one of said phycobiliproteins absorbs light in the range of about 300 to 600 nm and emits light in the range of about 400 to 700 nm and the other of said phycobiliproteins absorbs light in the range of about 400 to 650 nm and emits light in the range of about 500 to 800 nm, wherein there is substantial overlap between the emission spectrum of one and the absorption spectrum of the other to provide a spectral overlap integral of at least about 10 -16 cm 3 M -1 .
Claim 9
Dependent Claim
102 words
A conjugate comprising two phycobiliproteins joined within energy exchanging distance, wherein one of said phycobiliproteins absorbs light in the range of about 300 to 600 nm and emits light in the range of about 400 to 700 nm and the other of said phycobiliproteins absorbs light in the range of about 400 to 650 nm and emits light in the range of about 500 to 800 nm, wherein there is substantial overlap between the emission spectrum of one and the absorption spectrum of the other to provide a spectral overlap integral of at least about 10 -16 cm 3 M -1 .
Claim 10
Dependent Claim
21 words
A conjugate according to claim 7, wherein one of said phycobiliproteins is phycoerythrin and the other of said phycobiliproteins is allophycocyanin.
Claim 11
Dependent Claim
19 words
A conjugate according to claim 8, wherein said phycobiliproteins are joined by a linking group containing a disulfide linkage.
Claim 12
Dependent Claim
18 words
A conjugate comprising an energy donor-acceptor pair according to claim 1 covalently joined to a ligand or receptor.
Claim 13
Dependent Claim
56 words
In a cell staining, fluorescence-activated cell sorting or competitive protein binding diagnostic assay method employing a fluorescent label, where a labeled ligand and/or receptor are brought together and fluorescence is detected in relation to the binding of said ligand and receptor, the improvement comprising employing the conjugate composition according to claim 1 as the fluorescent label.
Claim 14
Dependent Claim
14 words
A method according to claim 11, wherein said method is fluorescence activated cell sorting.
Claim 15
Dependent Claim
17 words
A method according to claim 11, wherein said method is a diagnostic assay employing a fluorescent label.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 1988-09-16 | 1989-03-18 | 1989-09-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-16 | 1993-03-18 | 1993-09-17 | $3760.00 | Window Closed / Lapsed |
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