US4133873A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of determining extracellular antigens and antibodies
Assignee: Individual
•
Filing: 1976-05-24
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Granted: 1979-01-09
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Expires: 1996-05-24
Abstract
Abstract Hepatitis associated antigen (HAA) in human blood serum is precipitated on HAA-specific antibodies immobilized on the inner wall of a plastic tube. A solution of HAA-specific antibodies tagged with fluorescein is held in contact with the deposited antigens until a portion of the antibodies from the solution is affixed to the antigens. The residual, tagged antibodies in the solution are exposed to a light pulse, and the resulting fluorescence is measured as an inverse indication of HAA in the blood serum.
Inventors
- 👤 Hans G. Noller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 19:00
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N33/58; G01N33/582; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/807; Y10S436/81; Y10S436/82
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
115 words
A method of determining the amount of a member of the group consisting of an extracellular antigen and an extracellular antibody capable of specifically combining with said antigen, which comprises: (a) tagging said member with a fluorescent material; (b) exposing the tagged member to a pulse of light of a first wavelength, (1) the intensity and duration of said pulse being sufficient to cause the emission of secondary light by the tagged exposed member without causing significant decomposition of said material, (2) said secondary light having a second wavelength different from said first wavelength; (c) sensing said secondary light; and (d) generating a perceptible signal in response to and commensurate with the sensed secondary light.
Claim 2
Independent Claim
115 words
A method of determining the amount of a member of the group consisting of an extracellular antigen and an extracellular antibody capable of specifically combining with said antigen, which comprises: (a) tagging said member with a fluorescent material; (b) exposing the tagged member to a pulse of light of a first wavelength, (1) the intensity and duration of said pulse being sufficient to cause the emission of secondary light by the tagged exposed member without causing significant decomposition of said material, (2) said secondary light having a second wavelength different from said first wavelength; (c) sensing said secondary light; and (d) generating a perceptible signal in response to and commensurate with the sensed secondary light.
Claim 3
Dependent Claim
18 words
A method as set forth in claim 1, wherein said duration is 10 -3 to 10 -5 second.
Claim 4
Dependent Claim
27 words
A method as set forth in claim 2, wherein said tagged member is present as a solute in an aqueous medium while being exposed to said pulse.
Claim 5
Dependent Claim
29 words
A method as set forth in claim 3, wherein said tagged member is in the solid state and supported on a solid carrier while being exposed to said pulse.
Claim 6
Dependent Claim
25 words
A method as set forth in claim 4, wherein said carrier and the tagged member supported thereon are immersed in a liquid during said exposing.
Claim 7
Dependent Claim
14 words
A method as set forth in claim 2, wherein said member is an antibody.
Claim 8
Dependent Claim
86 words
A method as set forth in claim 6, wherein an aqueous solution of said tagged antibody is contacted prior to said exposing with said antigen while said antigen is immobilized on a solid carrier, the amount of said tagged antibody in said solution being sufficient to permit combining of a first portion of said antibody with said antigen, whereby said portion is immobilized on said carrier, while a second portion of said antibody remains in said solution, one of said portions being exposed to said pulse.
Claim 9
Dependent Claim
16 words
A method as set forth in claim 7, wherein the exposed portion is said second portion.
Claim 10
Dependent Claim
15 words
A method as set forth in claim 7, wherein said antigen is hepatitis associated antigen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-01-08 | 1982-07-10 | 1983-01-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-01-08 | 1986-07-10 | 1987-01-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-01-08 | 1990-07-10 | 1991-01-09 | $7700.00 | Window Closed / Lapsed |