US4757012A
Expired / Lapsed
US
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Ascorbic acid intermediates and process enzymes
Assignee: Genentech Inc
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Filing: 1984-06-14
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Granted: 1988-07-12
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Expires: 2004-06-14
Abstract
Abstract Recombinant plasmids containing the gene encoding 2,5-diketogluconic acid reductase are prepared and used to transform microorganisms. 2,5,DKG reductase is expressed by the microorganisms.
Inventors
- 👤 David A. Estell
- 👤 Robert A. Lazarus
- 👤 David R. Light
- 👤 Jeffrey V. Miller
- 👤 William H. Rastetter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 16:25
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; C12N; C12N9/00; C12N9/0004; C12N9/0006; C12Y; C12Y101/00; C12Y101/01; C12Y101/01274
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
42 words
A process for producing a recombinant microorganism capable of converting glucose to 2-KLG which comprises transforming a host cell capable of converting glucose to 2,5-DKG but lacking the ability to convert 2,5-DKG to 2-KLG with an expression vector which encodes 2,5-DKG reductase.
Claim 2
Dependent Claim
42 words
A process for producing a recombinant microorganism capable of converting glucose to 2-KLG which comprises transforming a host cell capable of converting glucose to 2,5-DKG but lacking the ability to convert 2,5-DKG to 2-KLG with an expression vector which encodes 2,5-DKG reductase.
Claim 3
Dependent Claim
14 words
The process of claim 1 wherein said host cell is of the genus Erwinia.
Claim 4
Dependent Claim
14 words
The process of claim 1 wherein said host cell is Erwinia herbicola (ATCC 21998).
Claim 5
Dependent Claim
13 words
A recombinant plasmid comprising a DNA segment encoding the enzyme 2,5 DKG reductase.
Claim 6
Dependent Claim
13 words
A recombinant plasmid comprising a DNA segment encoding the enzyme 2,5 DKG reductase.
Claim 7
Dependent Claim
25 words
The plasmid of claim 4 wherein the enzyme is one which migrates in reducing or non-reducing SDS PAGE corresponding to a M.W. of approximately 34,000.
References Cited (Backward Citations)
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Cited
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Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-07-12 | 1992-01-11 | 1992-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-07-12 | 1996-01-11 | 1996-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-07-12 | 2000-01-11 | 2000-07-12 | $7700.00 | Window Closed / Lapsed |