EP0046284A2
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EP
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Method for preparing 2,5-diketo-D-gluconic acid and microorganisms for carrying out the method
Assignee: Shionogi and Co Ltd
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Filing: 1981-08-14
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Granted: —
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Expires: 2001-08-14
Abstract
Abstract 2,5-Diketo-D-gluconic acid is prepared in high yield and in high broth concentration by cultivating newly isolated microorganisms of genus Erwinia in an aqueous nutrient medium in the presence of D-glucose. The production is also possible by simple contact of said microorganisms or their processed products therefrom, with D-glucose.
Inventors
- 👤 Takayasu Sonoyama
- 👤 Shigeo Yagi
- 👤 Bunji Kageyama
- 👤 Masahiro Tanimoto
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-04 22:39
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/847
Claims Analysis
Total Claims: 32
Claim 1
Independent Claim
59 words
A method for preparing 2,5-diketo-D-gluconic acid or a salt thereof which comprises converting D-glucose into 2,5-- diketo-D-gluconic acid in the presence of at least one strain of 2,5-diketo-D-gluconic acid producing microorganisms of the genus Erwinia or any processed product of said microorganism under conditions sufficient to produce and accumulate said acid or any salts thereof in a contacting mixture.
Claim 2
Independent Claim
59 words
A method for preparing 2,5-diketo-D-gluconic acid or a salt thereof which comprises converting D-glucose into 2,5-- diketo-D-gluconic acid in the presence of at least one strain of 2,5-diketo-D-gluconic acid producing microorganisms of the genus Erwinia or any processed product of said microorganism under conditions sufficient to produce and accumulate said acid or any salts thereof in a contacting mixture.
Claim 3
Dependent Claim
24 words
A method as claimed in Claim 1, wherein 2,5-diketo-D-gluconic acid or any salts thereof accumulated during the conversion are recovered from the resultant mixture.
Claim 4
Independent Claim
21 words
A method as claimed in Claim.1, wherein the concentration of D-glucose in the contacting mixture is up to 40 w/w %.
Claim 5
Dependent Claim
16 words
A method as claimed in Claim 1, wherein the conversion is conducted in an aerating condition.
Claim 6
Dependent Claim
19 words
A method as claimed in Claim 1, wherein the conversion is conducted at a temperature of 15-35 °C -
Claim 7
Dependent Claim
21 words
A method as claimed in Claim 1, wherein the pH of the contacting mixture is maintained at 4.0-7.5 during the conversion.
Claim 8
Dependent Claim
43 words
A method as claimed in Claim 1, wherein at least one strain of said microorganisms is cultivated in an aqueous nutrient medium in the presence of D-glucose to produce and accumulate 2,5-diketo-D-gluconic acid or any salts thereof in the medium during the cultivation.
Claim 9
Dependent Claim
39 words
A method as claimed in Claim 1, wherein at least one proces.sed product of said microorganisms is contacted with a substrate containing D-glucose to produce and accumulate 2,5-diketo-D-gluconic acid or any salts thereof in the substrate during the contact.
Claim 10
Dependent Claim
18 words
A method as claimed in Claim 1, wherein said processed product is a cell suspension of said microorganisms.
Claim 11
Dependent Claim
18 words
A method as claimed in Claim 1, wherein said processed product is an enzyme extracted from said microorganisms.
Claim 12
Dependent Claim
18 words
A method as claimed in Claim 1, wherein said microorganism is a strain of Erwinia citreus ATCC 31623.
Claim 13
Dependent Claim
22 words
A method as claimed in Claim 1, wherein said microorganism is a strain of Erwinia punctata ATCC 31624, 31625, 31626 or 31627.
Claim 14
Dependent Claim
22 words
A method as claimed in Claim 1, wherein said microorganism is a strain of Erwinia terreus ATCC 31628, 31629, 31630 or 31631.
Claim 15
Dependent Claim
4 words
Erwinia citreus ATCC 31623.
Claim 16
Dependent Claim
4 words
Erwinia citreus ATCC 31623.
Claim 17
Dependent Claim
4 words
Erwinia punctata ATCC 31624.
Claim 18
Dependent Claim
4 words
Erwinia punctata ATCC 31624.
Claim 19
Dependent Claim
4 words
Erwinia punctata ATCC 31625.
Claim 20
Dependent Claim
4 words
Erwinia punctata ATCC 31625.
Claim 21
Dependent Claim
4 words
Erwinia punctata ATCC 31626.
Claim 22
Dependent Claim
4 words
Erwinia punctata ATCC 31626.
Claim 23
Dependent Claim
4 words
Erwinia punctata ATCC 31627.
Claim 24
Dependent Claim
4 words
Erwinia punctata ATCC 31627.
Claim 25
Dependent Claim
4 words
Erwinia terreus ATCC 31628.
Claim 26
Dependent Claim
4 words
Erwinia terreus ATCC 31628.
Claim 27
Dependent Claim
4 words
Erwinia terreus ATCC 31629.
Claim 28
Dependent Claim
4 words
Erwinia terreus ATCC 31629.
Claim 29
Dependent Claim
4 words
Erwinia terreus ATCC 31630.
Claim 30
Dependent Claim
4 words
Erwinia terreus ATCC 31630.
Claim 31
Dependent Claim
4 words
Erwinia terreus ATCC 31631.
Claim 32
Dependent Claim
4 words
Erwinia terreus ATCC 31631.
References Cited (Backward Citations)
No citation records indexed.
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