EP0088408A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Process for preparing 2-keto-L-gulonic acid and mutants therefor
Assignee: Shionogi and Co Ltd
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Filing: 1983-03-04
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Granted: —
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Expires: 2003-03-04
Abstract
Abstract A fermentative or enzymatic production of 2-keto-L-gu-Ionic acid from 2,5-diketo-D-giuconic acid using living or processed mutants, being defective in metabolizing 5-keto-D-gluconic acid and incapable of producing 2-keto-D-gluconic acid, is disclosed. The mutant is derived from 2-keto-L-gluconic acid producing microorganisms of the genus Corynebacterium. The production is carried out in the presence of nitrates and/or hydrogen donors in a preliminarily sterilized fermentation broth in which a 2,5-diketo-D-gluconic acid producing microorganism of genus Gluconobacter or Erwinia has been cultivated.
Inventors
- 👤 Takayasu Sonoyama
- 👤 Shigeo Yagi
- 👤 Bunji Kageyama
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-05 18:11
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/15; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/843; Y10S435/847
Claims Analysis
Total Claims: 25
Claim 1
Independent Claim
50 words
In the process for preparing 2-keto-L-gulonic acid from 2,5-diketo-D-gluconic acid in the presence of a living or processed microorganism capable of producing 2- keto-L-gulonic acid, an improvement which comprises using being a mutant (1) being substantially defective in metabolizing 5- keto-D-gluconic acid (2) and substantially incapable of producing 2-keto-D-gluconic acid.
Claim 2
Dependent Claim
50 words
In the process for preparing 2-keto-L-gulonic acid from 2,5-diketo-D-gluconic acid in the presence of a living or processed microorganism capable of producing 2- keto-L-gulonic acid, an improvement which comprises using being a mutant (1) being substantially defective in metabolizing 5- keto-D-gluconic acid (2) and substantially incapable of producing 2-keto-D-gluconic acid.
Claim 3
Dependent Claim
16 words
A process as claimed in Claim 1, wherein said mutant belongs to Coryneform Group of Bacteria.
Claim 4
Dependent Claim
14 words
A process as claimed in Claim 1, wherein said mutant the belongs to/genus Corynebacterium.
Claim 5
Dependent Claim
15 words
A process as claimed in Claim 1, wherein said mutant is Corynebacterium sp. FERM-BP 107.
Claim 6
Dependent Claim
15 words
A process as claimed in Claim 1, wherein said mutant is Corynebacterium sp. FERM-BP 108.
Claim 7
Dependent Claim
23 words
A process as claimed in Claim 1, wherein said mutant is cultivated in an aqueous nutrient medium to which 2,5-diketo-D-gluconic acid is fed.
Claim 8
Dependent Claim
40 words
A process as claimed in Claim 6, wherein said 2,5-diketo-D-gluconic acid is prepared by cultivating a 2,5-diketo-D-gluconic acid producing microorganism which belongs the to/genus Gluconobacter or Erwinia in a nutrient medium a containing D-glucose in the form of fermentation broth.
Claim 9
Dependent Claim
69 words
A process as claimed in Claim 7, wherein said microorganism is selected from the group consisting of Acetomonas albosesamea ATCC 21998, Gluconobacter melanogenum IFO 3293, Gluconobacter rubisinosus IFO 3244, Erwinia citreus ATCC 31623, Erwinia punctata ATCC 31626, Erwinia punctata var.ATCC 31264, Erwinia punctata var.ATCC 31625, Erwinia punctata var. ATCC 31627, Erwinia terreus ATCC 31628, Erwinia terreus var. ATCC 31629, Erwinia terreus var. ATCC 31630.and Erwinia terreus var. ATCC 31631.
Claim 10
Dependent Claim
35 words
A process as claimed in Claim 1, wherein the growth of said mutant and the conversion of 2,5-diketo-D-gluconic acid into 2-keto-L-gulonic acid are effected in the presence of a nitrate salt or a hydrogen donor.
Claim 11
Dependent Claim
23 words
A process as claimed in Claim 9, wherein said nitrate nitrate salt./ salt is an alkali metal or an alkaline earth metal /
Claim 12
Dependent Claim
17 words
A process as claimed in Claim 9, wherein said nitrate salt is sodium nitrate or potassium nitrate.
Claim 13
Dependent Claim
20 words
A process as claimed in Claim 9, wherein the concentration of said nitrate salt is 0.05 - 0.5 w/v %.
Claim 14
Dependent Claim
22 words
A process as claimed in Claim 9, wherein said hydrogen donor is selected from the group consisting of carbohydrates and polyhydric alcohols.
Claim 15
Dependent Claim
25 words
A process as claimed in Claim 9 , wherein said hydrogen donor is selected from the group consisting of glycerol, glucose, fructose, sucrose and molasses.
Claim 16
Dependent Claim
24 words
A process as claimed in Claim 9, wherein said hydrogen donor is present in an amount of 5 -50 % of the 2,5-diketo-D-gluconic acid.
Claim 17
Dependent Claim
20 words
A process as claimed in Claim 7, wherein said 2,5-diketo-D-gluconic acid fermentation broth is sterilized with a surfactant before feeding.
Claim 18
Dependent Claim
15 words
A process as claimed in Claim 16, wherein said surfactant is sodium dodecyl sulfate (SDS).
Claim 19
Dependent Claim
20 words
A process as claimed in Claim 17, wherein said SDS is added at a concentration of 0.01 - 0.25 w/v
Claim 20
Dependent Claim
19 words
A process as claimed in Claim 1, wherein said microorganism is used in the form of a cell suspension.
Claim 21
Dependent Claim
19 words
A process as claimed in Claim 1, wherein said microorganism is used in the form of a cell extract.
Claim 22
Dependent Claim
50 words
A mutant for performing the process according to any of claims 1 to 20, characterized in that it is substantially defective in metabolizing 5-keto-D-gluconic acid and substantially incapable of producing 2-keto-D-gluconic acid, from which has been derived/a parent, a 2-keto-L-gulonic acid producing microorganism which belongs to Coryneform Group of Bacteria.
Claim 23
Dependent Claim
16 words
A mutant as claimed in Claim 21, characterized in that said parent belongs to genus Corynebacterium.
Claim 24
Dependent Claim
9 words
Corynebacteriucu sp. FERM-BP 107, as claimed in Claim 21.
Claim 25
Dependent Claim
9 words
Corynebacterium sp. FERM-BP 108, as claimed in Claim 21.
References Cited (Backward Citations)
No citation records indexed.
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