USRE30872E
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing 2-keto-L-gulonic acid
Assignee: Shionogi and Co Ltd
•
Filing: 1980-01-22
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Granted: 1982-02-23
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Expires: 2000-01-22
Abstract
Abstract 2-Keto-L-gulonic acid is prepared from 2,5-diketo-D-gluconic acid through microbial conversion. The 2-keto-L-gulonic acid producing microorganism used for this microbial conversion includes strains which belong to genera of Brevibacterium, Arthrobacter, Micrococcus, Staphylococcus, Pseudomonas and Bacillus. Both the incubation of the microorganism in a medium containing 2,5-diketo-L-gluconic acid and the direct contact of any products obtained from the cells with a substrate containing said 2,5-diketo-D-gluconic acid may be used in the disclosed process.
Inventors
- 👤 Takayasu Sonoyama
- 👤 Bunji Kageyama
- 👤 Tahiko Honjo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 00:36
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/40; C12P7/58; C12P7/60; C07; C07C; C07C59/00; C07C59/185; C07C59/215; C07H; C07H7/00; C07H7/02; C07H7/027; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/832; Y10S435/837; Y10S435/84; Y10S435/859; Y10S435/874; Y10S435/882; Y10S435/883
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
62 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises; contacting a 2-keto-L-gulonic acid producing strain selected from microorganisms which belong to the genera of Brevibacterium, .[.Arthrobacter,.]. Bacillus, Staphylococcus, Micrococcus and Pseudomonas, with 2,5-diketo-D-gluconic acid or a salt thereof, under conditions sufficient to produce 2-keto-L-gulonic acid, and recovering the produced 2-keto-L-gulonic acid or any salts thereof from the resultant mixture.
Claim 2
Dependent Claim
62 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises; contacting a 2-keto-L-gulonic acid producing strain selected from microorganisms which belong to the genera of Brevibacterium, .[.Arthrobacter,.]. Bacillus, Staphylococcus, Micrococcus and Pseudomonas, with 2,5-diketo-D-gluconic acid or a salt thereof, under conditions sufficient to produce 2-keto-L-gulonic acid, and recovering the produced 2-keto-L-gulonic acid or any salts thereof from the resultant mixture.
Claim 3
Dependent Claim
46 words
A process as claimed in claim 1, wherein the 2-keto-L-gulonic acid producing strain is incubated in a medium containing 2,5-diketo-D-gluconic acid or a salt thereof to effect a fermentative action therein, and recovering 2-keto-L-gulonic acid or any salts thereof accumulated during the incubation from the medium.
Claim 4
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Brevibacterium.
Claim 5
Dependent Claim
15 words
A process as claimed in claim 3, wherein said strain belongs to Brevibacterium ketosoreductum. .[.
Claim 6
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Artheofacter..].
Claim 7
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Bacillus.
Claim 8
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Staphylococcus.
Claim 9
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Micrococcus.
Claim 10
Dependent Claim
19 words
A process as claimed in claim 2, wherein said 2-keto-L-gulonic acid producing strain belongs to the genus of Pseudomonas.
Claim 11
Dependent Claim
55 words
A process as claimed in claim 1, wherein cells of said microorganism strain are contacted with a substance containing 2,5-diketo-D-gluconic acid or a salt thereof under conditions sufficient to produce 2-keto-L-gulonic acid, and the 2-keto-L-gulonic acid or any salts thereof produced and accumulated as a result of the contact are recovered from the resultant mixture.
Claim 12
Dependent Claim
29 words
A process as claimed in claim 1, wherein an aqueous medium containing viable cells of said microorganism is contacted with a substance containing 2,5-diketo-D-gluconic acid or a salt thereof.
Claim 13
Dependent Claim
71 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises cultivating 2-keto-L-gulonic acid producing microorganisms selected from the group consisting of Brevibacterium ketosoreductum, .[.Arthrobacter simplex,.]. Bacillus megaterium, Staphylococcus aureus, Micrococcus dinitrificans, Micrococcus rubens, Micrococcus roseus and Pseudomonas chlororaphis, and mutants thereof, in an aqueous nutrient medium in the presence of 2,5-diketo-D-gluconic acid or a salt thereof, and recovering the produced 2-keto-L-gulonic acid or salts thereof from the resultant mixture.
Claim 14
Dependent Claim
71 words
A process for producing 2-keto-L-gulonic acid or a salt thereof which comprises cultivating 2-keto-L-gulonic acid producing microorganisms selected from the group consisting of Brevibacterium ketosoreductum, .[.Arthrobacter simplex,.]. Bacillus megaterium, Staphylococcus aureus, Micrococcus dinitrificans, Micrococcus rubens, Micrococcus roseus and Pseudomonas chlororaphis, and mutants thereof, in an aqueous nutrient medium in the presence of 2,5-diketo-D-gluconic acid or a salt thereof, and recovering the produced 2-keto-L-gulonic acid or salts thereof from the resultant mixture.
Claim 15
Dependent Claim
30 words
A process as claimed in claim 12, wherein said microorganism is Brevibacterium ketosoreductum ATCC 21914. .[.14. A process as claimed in claim 12, wherein said microorganism is Arthrobacter simplex ATCC
Claim 16
Dependent Claim
12 words
.]. 15. A process as claimed in claim 12, wherein said microorganism
Claim 17
Dependent Claim
21 words
is Bacillus megaterium ATCC 21916. 16. A process as claimed in claim 12, wherein said microorganism is Staphylococcus aureus ATCC 21915.
References Cited (Backward Citations)
US2741577A
Cited
US3076750A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-02-22 | 1985-08-24 | 1986-02-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-02-22 | 1989-08-24 | 1990-02-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-02-22 | 1993-08-24 | 1994-02-23 | $7700.00 | Window Closed / Lapsed |