US4751190A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Fluorescence polarization immunoassay and reagents for use therein
Assignee: Abbott Laboratories
•
Filing: 1986-04-03
•
Granted: 1988-06-14
•
Expires: 2006-04-03
Abstract
Abstract An method of carrying out a fluorescence polarization immunoassay is disclosed wherein the immunoassay is conducted in the presence of from about 0.001 to about 1.0 percent (weight/volume) of dioctyl sodium sulfosuccinate. Also disclosed are reagents useful in the practice of the immunoassay method.
Inventors
- 👤 Enrico G. Chiapetta
- 👤 Robert J. Kucera
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 16:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/536; G01N33/542; G01N33/58; G01N33/582; G01N33/68; G01N33/6893; G01N33/72; G01N33/728; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/805; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
70 words
In a method of determining the presence or amount of a ligand in a sample by fluorescence polarization immunoassay comprising adding a tracer and an antibody specific for said ligand and observing a polarization value as an indication of the amount of ligand in the sample the improvement comprising: conducting the fluorescent polarization immunoassay in the presence of from about 0.001 to about 1.0 percent (weight/volume) of dioctyl sodium sulfosuccinate.
Claim 2
Dependent Claim
70 words
In a method of determining the presence or amount of a ligand in a sample by fluorescence polarization immunoassay comprising adding a tracer and an antibody specific for said ligand and observing a polarization value as an indication of the amount of ligand in the sample the improvement comprising: conducting the fluorescent polarization immunoassay in the presence of from about 0.001 to about 1.0 percent (weight/volume) of dioctyl sodium sulfosuccinate.
Claim 3
Dependent Claim
27 words
The method of claim 1 wherein the fluorescence polarization immunoassay is conducted in the presence of about 0.002 to about 0.5 percent (weight/volume) of dioctyl sodium sulfosuccinate.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein the fluorescence polarization immunoassay is conducted in the presence of about 0.005 to about 0.1 percent so dioctyl sodium sulfosuccinate.
References Cited (Backward Citations)
US3864317A
Cited
DE2323605A1
Cited
US3873721A
Cited
US4119401A
Cited
US4492762A
Cited
US4492762B1
Cited
WO1986000933A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-06-14 | 1991-12-14 | 1992-06-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-06-14 | 1995-12-14 | 1996-06-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-06-14 | 1999-12-14 | 2000-06-14 | $7700.00 | Window Closed / Lapsed |