US4119401A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Total bilirubin assay
Assignee: Bayer Corp
•
Filing: 1977-06-07
•
Granted: 1978-10-10
•
Expires: 1997-06-07
Abstract
Abstract Reagent and reagent mixtures useful for detecting and measuring total bilirubin concentrations in biological liquids such as serum and methods of assaying biological liquids using such reagents are disclosed.
Inventors
- 👤 Musa M. Sansur
- 👤 Daniel L. Vlastelica
- 👤 Anne C. Delea
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 07:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/728; Y; Y10; Y10S; Y10S436/00; Y10S436/903; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
56 words
A method for quantitatively determining the total bilirubin concentration in a bilirubin-containing biological liquid which comprises at least partially solubilizing a measured amount of said biological liquid under alkaline conditions, reacting the liberated bilirubin from said biological liquid with a stabilized diazo reagent under said alkaline conditions and spectrophotometrically measuring absorbance of the resulting solubilized mixture.
Claim 2
Independent Claim
56 words
A method for quantitatively determining the total bilirubin concentration in a bilirubin-containing biological liquid which comprises at least partially solubilizing a measured amount of said biological liquid under alkaline conditions, reacting the liberated bilirubin from said biological liquid with a stabilized diazo reagent under said alkaline conditions and spectrophotometrically measuring absorbance of the resulting solubilized mixture.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein said spectrophotometric measurement is read at about 600 mu.
Claim 4
Dependent Claim
15 words
The method of claim 1 wherein said treatment and reaction steps are carried out simultaneously.
Claim 5
Dependent Claim
25 words
The method of claim 1 wherein said mixture containing said biological liquid is rendered solubilized by the combined effect of sodium hydroxide, glycine and dyphylline.
Claim 6
Dependent Claim
25 words
The method of claim 1 wherein the reagents used to effect said partial solubilization and said reaction are maintained under lyophilized conditions prior to use.
Claim 7
Dependent Claim
30 words
The method of claim 1 wherein the reagents used to effect said partial solubilization and said reaction are reconstituted just prior to use in said partial solubilization and reaction steps.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein said treatment and reaction steps are carried out sequentially.
Claim 9
Dependent Claim
51 words
The method of claim 7 wherein said partial solubilization step comprises treating a measured amount of said biological liquid with a mixture comprised of sodium hydroxide and glycine, and said reaction step comprises mixing said partially solubilized liquid with a mixture comprised of sulfanilic acid, sodium nitrite, maleic acid and dyphylline.
Claim 10
Dependent Claim
16 words
The method of claim 8 wherein said partial solubilization and reaction mixtures are in lyophilized form.
References Cited (Backward Citations)
US2770602A
Cited
US3485587A
Cited
US3171783A
Cited
US3438737A
Cited
US3874794A
Cited
US4038031A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-10-09 | 1982-04-10 | 1982-10-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-10-09 | 1986-04-10 | 1986-10-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-10-09 | 1990-04-10 | 1990-10-10 | $7700.00 | Window Closed / Lapsed |