US4038031A
Expired / Lapsed
US
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Test composition, device and method for detecting bilirubin
Assignee: Bayer Corp
•
Filing: 1975-10-02
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Granted: 1977-07-26
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Expires: 1995-10-02
Abstract
Abstract A test composition, device, and method for detecting bilirubin in urine. The test composition includes a diazonium compound reactable with urinary bilirubin to produce a color change, an acid pH producing constituent, and a potentiating agent for the test reaction. The potentiating agent is an adduct of a ureido compound and an organic sulfonic acid or a salt form thereof. Particularly useful as the ureido constituent of the adduct are urea and substituted and unsubstituted cyclic ureido compounds, particularly those belonging to the xanthine family. The test device comprises a carrier, such as an absorbent matrix, incorporated with the test composition. The test method is carried out by contacting a urine test sample with the test composition and observing any resulting colorimetric response.
Inventors
- 👤 Charles T. W. Lam
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:35
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/728; Y; Y10; Y10S; Y10S436/00; Y10S436/903; Y10T; Y10T436/00; Y10T436/14; Y10T436/145555; Y10T436/146666
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
77 words
A test composition for detecting bilirubin in a urine sample, which composition comprises a diazonium compound which reacts with urinary bilirubin to produce a color change; a constituent capable of producing an acidic pH in said urine sample; and an organic sulfonic acid or an organic sulfonic acid salt adduct of urea or of a derivative of urea, which adduct has a potentiating effect on reaction of said diazonium compound with urinary bilirubin in an acidic environment.
Claim 2
Dependent Claim
77 words
A test composition for detecting bilirubin in a urine sample, which composition comprises a diazonium compound which reacts with urinary bilirubin to produce a color change; a constituent capable of producing an acidic pH in said urine sample; and an organic sulfonic acid or an organic sulfonic acid salt adduct of urea or of a derivative of urea, which adduct has a potentiating effect on reaction of said diazonium compound with urinary bilirubin in an acidic environment.
Claim 3
Dependent Claim
21 words
A test composition as claimed in claim 1 wherein said derivative of urea is an acyclic lower alkyl derivative of urea.
Claim 4
Dependent Claim
22 words
A test composition as claimed in claim 1 wherein said derivative of urea is a cyclic ureido compound or a derivative thereof.
Claim 5
Dependent Claim
59 words
A test composition as claimed in claim 3 wherein said cyclic ureido compound or derivative thereof has a dioxoheterocyclic ring structure of the formula ##STR5## or a tautomeric form thereof, wherein R is a linking group forming a 5 or 6 member ring and wherein the heterocyclic ring may contain substituents that do not substantially impair said potentiating effect.
Claim 6
Dependent Claim
19 words
A test composition as claimed in claim 1 wherein said derivative of urea is 2,6-dioxopyrimidine or a derivative thereof.
Claim 7
Dependent Claim
87 words
A test composition as claimed in claim 1 wherein said derivative of urea is selected from the group consisting of compounds having the formula ##STR6## or a tautomeric form thereof, wherein R 1 and R 2 are respectively hydrogen or a lower alkyl group and wherein R 3 and R 4 (1) are respectively hydrogen, halogeno, or a lower alkyl group or (2) together with the ethylene link in the heterocyclic ring form an isocyclic or heterocyclic ring system which does not substantially impair said potentiating effect.
Claim 8
Dependent Claim
25 words
A test composition as claimed in claim 6 wherein the organic sulfonic acid or the organic sulfonic acid salt constituent of said adduct is aromatic.
Claim 9
Dependent Claim
60 words
A test composition as claimed in claim 1 wherein said derivative of urea is selected from the group consisting of compounds having the formula ##STR7## or a tautomeric form thereof, wherein R 1 and R 2 are respectively hydrogen or a lower alkyl group and wherein R 3 is hydrogen, a lower alkyl group, or a hydroxy-substituted lower alkyl group.
Claim 10
Dependent Claim
25 words
A test composition as claimed in claim 8 wherein the organic sulfonic acid or the organic sulfonic acid salt constituent of said adduct is aromatic.
Claim 11
Dependent Claim
27 words
A test composition as claimed in claim 1 wherein said urea derivative is selected from the group consisting of caffeine, dyphylline, uridine, urazole, 2-imidazolidone, and parabanic acid.
Claim 12
Dependent Claim
25 words
A test composition as claimed in claim 10 wherein the organic sulfonic acid or the organic sulfonic acid salt constituent of said adduct is aromatic.
Claim 13
Dependent Claim
14 words
A test composition is claimed in claim 1 wherein said urea derivative is caffeine.
Claim 14
Dependent Claim
25 words
A test composition as claimed in claim 12 wherein the organic sulfonic acid or the organic sulfonic acid salt constituent of said adduct is aromatic.
Claim 15
Dependent Claim
31 words
A test composition as claimed in claim 12 wherein the organic sulfonic acid or the organic sulfonic acid salt constituent of said adduct is sulfosalicylic acid or a salt form thereof.
Claim 16
Dependent Claim
16 words
A test composition as claimed in claim 1 wherein said diazonium compound is an aryldiazonium salt.
Claim 17
Dependent Claim
16 words
A test composition as claimed in claim 15 wherein said aryldiazonium salt is a 2,4-dichlorobenzenediazonium salt.
Claim 18
Dependent Claim
25 words
A test composition as claimed in claim 1 wherein the organic sulfonic acid or the organic sulfonic acid salt constituent of said adduct is aromatic.
Claim 19
Dependent Claim
27 words
A test composition as claimed in claim 17 wherein said aromatic sulfonic acid is selected from the group consisting of sulfosalicylic acid, naphthalenedisulfonic acids, and biphenyldisulfonic acids.
Claim 20
Dependent Claim
25 words
A test composition as claimed in claim 1 wherein said constituent capable of producing an acidic pH in said urine sample is an organic acid.
Claim 21
Dependent Claim
21 words
A test composition as claimed in claim 19 wherein said organic acid has a pK a of less than about 4.
Claim 22
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 1 and a carrier therefor.
Claim 23
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 6 and a carrier therefor.
Claim 24
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 8 and a carrier therefor.
Claim 25
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 9 and a carrier therefor.
Claim 26
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 11 and a carrier therefor.
Claim 27
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 12 and a carrier therefor.
Claim 28
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 13 and a carrier therefor.
Claim 29
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 17 and a carrier therefor.
Claim 30
Dependent Claim
21 words
A test device for detecting bilirubin in urine, which device comprises the test composition of claim 18 and a carrier therefor.
Claim 31
Dependent Claim
29 words
A method for detecting bilirubin in urine, which method comprises contacting a urine test sample with the test composition of claim 1 and observing any colorimetric response which results.
Claim 32
Dependent Claim
28 words
A method for detecting bilirubin in urine, which method comprises contacting urine test sample with the test composition of claim 11 and observing any colorimetric response which results.
Claim 33
Dependent Claim
29 words
A method for detecting bilirubin in urine, which method comprises contacting a urine test sample with the test composition of claim 13 and observing any colorimetric response which results.
Claim 34
Dependent Claim
29 words
A method for detecting bilirubin in urine, which method comrises contacting a urine test sample with the test device of claim 22 and observing any colorimetric response which results.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-07-25 | 1981-01-24 | 1981-07-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-07-25 | 1985-01-24 | 1985-07-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-07-25 | 1989-01-24 | 1989-07-26 | $7700.00 | Window Closed / Lapsed |