US4698315A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and kit for determining total digoxin levels involving agent to dissociate digoxin-protein complex
Assignee: Hoffmann La Roche Inc
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Filing: 1985-04-22
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Granted: 1987-10-06
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Expires: 2005-04-22
Abstract
Abstract A method for determining total digoxin levels in a biological fluid sample containing digoxin-protein complex which method comprises disrupting the digoxin-protein complex by treating said sample solution with an effective amount of a dissociation agent selected from one or more members of the group consisting of a C3-C26 saturated or unsaturated fatty acid, a C2-7 lower alkanol, quinidine and alpha -tocopherol so as to release the bound digoxin from serum protein, passing the treated sample solution through a centrifugal ultrafilter so as to selectively pass digoxin containing sample solution through said filter and retaining said serum proteins and determining the digoxin contents in said sample solution in a fluorescence polarization assay. A kit for carrying out the method is provided.
Inventors
- 👤 Bruce C. Farrenkopf
- 👤 Richard A. Kaufman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 22:56
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/94; G01N33/9453; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/815; Y10S436/825; Y10T; Y10T436/00; Y10T436/25; Y10T436/25375
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
114 words
A method for determining total digoxin levels in a biological fluid sample containing digoxin-protein complex which method comprises disrupting the digoxin-protein complex by treating said sample solution with an effective amount of a dissociation agent selected from one or more members of the group consisting of a C 3 -C 26 saturated or unsaturated fatty acid, a C 2-7 lower alkanol, quinidine and α-tocopherol so as to release the bound digoxin from serum protein, passing the treated sample solution through a centrifugal ultrafilter so as to selectively pass digoxin containing sample solution through said filter and retaining said serum proteins and determining the digoxin contents in said sample solution in a fluorescence polarization assay.
Claim 2
Independent Claim
114 words
A method for determining total digoxin levels in a biological fluid sample containing digoxin-protein complex which method comprises disrupting the digoxin-protein complex by treating said sample solution with an effective amount of a dissociation agent selected from one or more members of the group consisting of a C 3 -C 26 saturated or unsaturated fatty acid, a C 2-7 lower alkanol, quinidine and α-tocopherol so as to release the bound digoxin from serum protein, passing the treated sample solution through a centrifugal ultrafilter so as to selectively pass digoxin containing sample solution through said filter and retaining said serum proteins and determining the digoxin contents in said sample solution in a fluorescence polarization assay.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein said dissociation agent is linolenic acid.
Claim 4
Dependent Claim
12 words
The method of claim 1 wherein said dissociation agent is arachadonic acid.
Claim 5
Dependent Claim
12 words
The method of claim 1 wherein said dissociation agent is linoleic acid.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein said dissociation agent is a mixture of isopropyl alcohol and linolenic acid.
Claim 7
Dependent Claim
12 words
The method of claim 1 wherein said dissociation agent is isopropyl alcohol.
Claim 8
Dependent Claim
47 words
A kit useful for determining total digoxin levels in a biological fluid sample, said kit comprising one bottle each of the following reagents in quantities sufficient to carry out multiple assays: (1) anti-digoxin antibody reagent solution; (2) Tracer reagent solution; and (3) digoxin-protein complex dissociating agent solution.
Claim 9
Dependent Claim
47 words
A kit useful for determining total digoxin levels in a biological fluid sample, said kit comprising one bottle each of the following reagents in quantities sufficient to carry out multiple assays: (1) anti-digoxin antibody reagent solution; (2) Tracer reagent solution; and (3) digoxin-protein complex dissociating agent solution.
Claim 10
Dependent Claim
13 words
The kit of claim 7 wherein said anti-digoxin antibody is rabbit anti-digoxin serum.
Claim 11
Dependent Claim
19 words
The kit of claim 7 wherein said digoxin-protein complex dissociating agent is a mixture of linolenic acid in isopropanol.
Claim 12
Dependent Claim
14 words
The kit of claim 7 wherein said tracer solution is a fluorescein digoxin conjugate.
Claim 13
Dependent Claim
45 words
The kit of claim 7 wherein (1) said anti-digoxin antibody is rabbit anti-digoxin serum; (2) said digoxin-protein complex dissociating agent is selected from the group consisting of linolenic acid, arachadonic acid and linoleic acid; and (3) digoxin tracer reagent solution is a fluorescein digoxin conjugate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-10-05 | 1991-04-06 | 1991-10-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-10-05 | 1995-04-06 | 1995-10-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-10-05 | 1999-04-06 | 1999-10-06 | $7700.00 | Window Closed / Lapsed |