EP0108403A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Substituted carboxyfluoresceins
Assignee: Abbott Laboratories
•
Filing: 1983-11-04
•
Granted: —
•
Expires: 2003-11-04
Abstract
Abstract This disclosure relates to a method and reagents for determining ligands in biological fluids such as serum, plasma, spinal fluid, amnionic fluid and urine. In particular, this disclosure relates to a fluorescence polarization immunoassay procedure and to a novel class of tracer compounds employed as reagents in such procedures.
Inventors
- 👤 Charles Arthur Flentge
- 👤 Curtis Lee Kirkemo
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-09 23:06
CPC Classifications
C; C07; C07D; C07D407/00; C07D407/02; C07D407/12; C07D311/00; C07D311/02; C07D311/78; C07D311/80; C07D311/82; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
49 words
A compound of the formula: or wherein T is a wherein n is an integer of from 1 to 8; and R is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; and biologically acceptable salts thereof.
Claim 2
Dependent Claim
49 words
A compound of the formula: or wherein T is a wherein n is an integer of from 1 to 8; and R is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; and biologically acceptable salts thereof.
Claim 3
Dependent Claim
12 words
A compound according to Claim 1 wherein R is a thyroxine analog.
Claim 4
Dependent Claim
9 words
A compound according to Claim 2 wherein R is
Claim 5
Dependent Claim
16 words
A. compound according to Claim 3 wherein n is an integer of from 2 to 4.
Claim 6
Dependent Claim
10 words
A compound according to Claim 4 wherein n is 3.
Claim 7
Dependent Claim
100 words
6, A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: 5 or wherein T is a wherein n is an integer of from 1 to 8; and R is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; and biologically acceptable salts thereof; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the'amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 8
Dependent Claim
100 words
6, A method for determining ligands in a sample comprising intermixing with said sample a tracer of the formula: 5 or wherein T is a wherein n is an integer of from 1 to 8; and R is a ligand-analog having at least one common epitope with a ligand so as to be specifically recognizable by a common antibody; and biologically acceptable salts thereof; and an antibody capable of specifically recognizing said ligand and said tracer; and then determining the'amount of tracer bound to antibody by fluorescence polarization techniques as a measure of the amount of ligand in the sample.
Claim 9
Dependent Claim
19 words
A method according to Claim 6 wherein R" has a molecular weight within a range of 50 to 4000.
Claim 10
Dependent Claim
12 words
A method according to Claim 7 wherein R is a thyroxine analog.
Claim 11
Dependent Claim
9 words
A method according to Claim 8 wherein R is
Claim 12
Dependent Claim
18 words
A method according to Claim 6 or 9 wherein n is an integer of from 2 to 4.
Claim 13
Dependent Claim
10 words
A method according to Claim 10 wherein n is 3.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.