EP0115332A2
Expired / Lapsed
EP
⏱️ 0.0 years remaining
Chromogenic tracers for use in an assay
Assignee: Becton Dickinson and Co
•
Filing: 1984-01-21
•
Granted: —
•
Expires: 2004-01-21
Abstract
Abstract A chromogen, in particular, a fluorescent compound, is conjugated to a ligand through an amino acid spacer radial, which includes a free carboxyl group. The tracers are particularly suitable for use in a flow through assay for low molecular weight analytes. The tracers are rapidly bound, of good sensitivity, and low non-specific binding.
Inventors
- 👤 Daniel B. Wagner
- 👤 Richard L. Tyson
Bibliographic Details
Application Number
—
Jurisdiction
EP
Data Source
google_patents
Last Synchronized
2026-11-10 01:59
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; G01N33/532; G01N33/533; G01N33/74; G01N33/78; G01N33/94; G01N33/9453; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/811; Y10S436/815; Y10S436/816; Y10S436/817
Claims Analysis
Total Claims: 34
Claim 1
Independent Claim
101 words
A tracer for use in an assay comprising: a chromogen conjugated to a ligand through a spacer radical, said spacer radical for conjugating the chromogen to the ligand being derived from an amino acid having the following structural formula: wherein X is selected from the group consisting of hydrogen, ammonium, amine salt and metal cations; Y is selected from the group consisting of -NH- and - and R is selected from the group consisting of substituted and unsubstituted divalent hydrocarbon radicals having no more than 13 carbon atoms and wherein -R- and -CH- may be linked to form a carbocyclic radical.
Claim 2
Dependent Claim
101 words
A tracer for use in an assay comprising: a chromogen conjugated to a ligand through a spacer radical, said spacer radical for conjugating the chromogen to the ligand being derived from an amino acid having the following structural formula: wherein X is selected from the group consisting of hydrogen, ammonium, amine salt and metal cations; Y is selected from the group consisting of -NH- and - and R is selected from the group consisting of substituted and unsubstituted divalent hydrocarbon radicals having no more than 13 carbon atoms and wherein -R- and -CH- may be linked to form a carbocyclic radical.
Claim 3
Dependent Claim
12 words
The tracer of Claim 1 wherein the chromogen is a fluorescein dye.
Claim 4
Dependent Claim
19 words
The tracer of Claim 2 wherein R is an alkylene group having 4 carbon atoms and Y is -NH-.
Claim 5
Dependent Claim
10 words
The tracer of Claim 3 wherein the ligand is triiodothyronine.
Claim 6
Dependent Claim
10 words
The tracer of Claim 3 wherein the ligand is thyroxine.
Claim 7
Dependent Claim
10 words
The tracer of Claim 3 wherein the ligand is digoxigenin.
Claim 8
Dependent Claim
9 words
The tracer of Claim 1 wherein R is alkylene.
Claim 9
Dependent Claim
12 words
The tracer of Claim 7 wherein the chromogen is a fluorescent dye.
Claim 10
Dependent Claim
9 words
The tracer of Claim 8 wherein Y is -NH-.
Claim 11
Dependent Claim
18 words
The tracer of Claim 9 wherein R is an alkylene group having from 2 to 6 carbon atoms.
Claim 12
Dependent Claim
13 words
The tracer of Claim 10 wherein the fluorescent dye is a fluorescein dye.
Claim 13
Dependent Claim
10 words
The tracer of Claim 1 wherein the tracer is N-a-(3-digoxigeninyl)-N-e-[5(6)-carboxyfluoresceinoyl]-lysine.
Claim 14
Dependent Claim
10 words
The tracer of Claim 1 wherein the tracer is carboxyfluorescein-lysylamide-3-ketodigoxigenin.
Claim 15
Dependent Claim
10 words
The tracer of Claim 1 wherein the tracer is carboxyfluorescein-lysylamide-3,3',5-triiodo-L-thyronine.
Claim 16
Dependent Claim
10 words
The tracer of Claim 1 wherein the tracer is carboxyfluorescein-lysylamide-3,3',5-triiodo-N-acetyl-L-thyronine.
Claim 17
Dependent Claim
10 words
The tracer of Claim 1 wherein the tracer is N-a-(3-digoxigeminyl-N-e-(dichlorotriazinylaminofluoresceinyl)-lysine.
Claim 18
Dependent Claim
34 words
In an assay for an analyte employing a tracer wherein a chromogen is conjugated to a ligand through a spacer radical, the improvement comprising: said tracer being a tracer as defined in Claim 1.
Claim 19
Dependent Claim
24 words
The assay of Claim 17 wherein during the assay the tracer is passed through a flow through chamber including a binder for the tracer.
Claim 20
Dependent Claim
12 words
The assay of Claim 18 wherein the chromogen is a fluorescein dye.
Claim 21
Dependent Claim
21 words
The assay of Claim 19 wherein R is an alkylene group having 4 carbon atoms and Y is - NH -.
Claim 22
Dependent Claim
25 words
The assay of Claim 20 wherein the ligand is triiodothyronine and wherein the assay is a T 3 uptake assay for T 3 serum binding.
Claim 23
Dependent Claim
18 words
2 2. The assay of Claim 20 wherein the ligand is thyroxine and wherein the analyte is thyroxine.
Claim 24
Dependent Claim
16 words
The assay of Claim 20 wherein, the ligand is digoxigenin and wherein the analyte is digoxin.
Claim 25
Dependent Claim
9 words
The assay of Claim 18 wherein R is alkylene.
Claim 26
Dependent Claim
12 words
The assay of Claim 24 wherein the chromogen is a fluorescent dye.
Claim 27
Dependent Claim
9 words
The assay of Claim 25 wherein Y is -NH-.
Claim 28
Dependent Claim
17 words
The assay of Claim 26 wherein R is an alkylene group'having from 2 to 6 carbon atoms.
Claim 29
Dependent Claim
13 words
The assay of Claim 27 wherein the fluorescent dye is a fluorescein dye.
Claim 30
Dependent Claim
16 words
The assay of Claim 18 wherein the tracer is carboxyfluorescein-lysylamide-N-acetyl-L-thyroxine and wherein the analyte is thyroxine.
Claim 31
Dependent Claim
16 words
The assay of Claim 18 wherein the tracer is N-a-(3-digoxigeninyl)-N-e-[5(6)-carboxyfluoresceinoyl]-lysine and wherein the analyte is digoxin.
Claim 32
Dependent Claim
26 words
The assay of Claim 18 wherein the tracer is carboxyfluorescein-lysylamide-3,3',5-triiodo-L-thyronine and wherein the assay is a T 3 uptake assay for T 3 serum binding proteins.
Claim 33
Dependent Claim
27 words
The assay of Claim 18 wherein the tracer is carboxyfluorescein-lysylamide-3,3'5-triiodo-N-acetyl-L- thyronine and wherein the assay is a T 3 uptake assay for T 3 serum binding proteins.
Claim 34
Dependent Claim
48 words
In a reagent kit for use in an assay for an analyte which includes a tracer wherein a chromogen is linked to a ligand through a spacer radical, and a binder for the tracer, the improvement comprising: said tracer being a tracer formula as defined in Claim 1.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
Maintenance schedule is calculated for granted US utility patents.