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US4680258A
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Process for sex determination in man by use of monoclonal antibodies to the H-Y antigen
Assignee: Memorial Sloan Kettering Cancer Center
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Filing: 1983-08-09
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Granted: 1987-07-14
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Expires: 2003-08-09
Abstract
Abstract Production of anti-H-Y hybridoma cell lines, and the use of the antibodies to determine the presence of H-Y antigen to indicate the sex of the proband inclusive of fetus, newborn and adult humans.
Inventors
- 👤 Ulrich Hammerling
- 👤 Gloria C. Koo
- 👤 Nobuhiko Tada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 10:41
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/18; C07K16/28; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S436/00; Y10S436/821; Y10S436/828
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
33 words
Hybridoma cell lines selected from the group consisting of ATCC HB 9070, ATCC HB 9071 and ATCC HB 9072 producing monoclonal antibodies 12/44, 12/49, and 11/6 which specifically bind to the H-Y antigen.
Claim 2
Dependent Claim
33 words
Hybridoma cell lines selected from the group consisting of ATCC HB 9070, ATCC HB 9071 and ATCC HB 9072 producing monoclonal antibodies 12/44, 12/49, and 11/6 which specifically bind to the H-Y antigen.
Claim 3
Dependent Claim
99 words
Process for determining gender of a fetus carried in a female, comprising obtaining a sample of blood or amniotic fluid from the female and measuring the H-Y antigen in said sample by contacting said sample with monoclonal anti-H-Y antibodies selected from the group consisting of 12/44, 12/49, and 6/11 produced by hybridoma cell line ATCC HB 9070, ATCC HB 9071 or ATCC HB 9072 and observing reaction between said sample and said monoclonal antibodies, presence of said reaction indicating that the gender of said fetus is male absence of said reaction indicating the gender of said fetus is female.
Claim 4
Dependent Claim
99 words
Process for determining gender of a fetus carried in a female, comprising obtaining a sample of blood or amniotic fluid from the female and measuring the H-Y antigen in said sample by contacting said sample with monoclonal anti-H-Y antibodies selected from the group consisting of 12/44, 12/49, and 6/11 produced by hybridoma cell line ATCC HB 9070, ATCC HB 9071 or ATCC HB 9072 and observing reaction between said sample and said monoclonal antibodies, presence of said reaction indicating that the gender of said fetus is male absence of said reaction indicating the gender of said fetus is female.
Claim 5
Dependent Claim
38 words
Process of claim 2 wherein the step of determining whether or not a reaction occurs comprises a direct radioimmuno assay using radioactive labeled monoclonal anti-H-Y antibodies, or an indirect assay, using unlabeled anti-H-Y antibody and radioactively labeled anti-immunoglobulin.
Claim 6
Dependent Claim
22 words
Process of claim 2 wherein the step of determining whether or not a reaction occurs comprises a Protein A rosette binding assay.
Claim 7
Dependent Claim
15 words
Process of claim 2 wherein said reaction is detected by enzyme-linked antibody binding assay (ELISA).
Claim 8
Dependent Claim
23 words
Process of claim 2 wherein said reaction is detected by an assay selected from the group consisting of direct and indirect immunofluorescence assays.
Claim 9
Dependent Claim
20 words
Process of claim 3 wherein the step of determining whether or not a reaction occurs comprises a complement-dependent cytotoxicity reaction.
Claim 10
Dependent Claim
35 words
Process of claim 2 wherein the step of determining whether or not a reaction occurs comprises a neutralization assay with cell bound H-Y antigen and further comprises measurement of whether neutralization of anti-H-Y antibody occurs.
Claim 11
Dependent Claim
32 words
Process of claim 3 wherein said sample comprises human amniotic cells whereby the presence of H-Y positive cells indicates a male fetus and the absence of H-Y positive cells a female fetus.
Claim 12
Dependent Claim
19 words
Process of claim 9 wherein the step of determining whether a reaction occurs comprises an immunofluorescence flow cytometry assay.
Claim 13
Dependent Claim
29 words
Process of claim 2 wherein said sample comprises human amniotic fluid whereby the presence of H-Y antigen signals a male fetus and the absence of H-Y a female fetus.
Claim 14
Dependent Claim
26 words
Process of claim 2 or 8 wherein said sample comprises serum of a pregnant woman, whereby the presence of H-Y antigen will indicate a male fetus.
Claim 15
Dependent Claim
27 words
Process of claim 2 wherein said sample comprises peripheral blood cells of a pregnant woman, whereby the presence of H-Y positive cells with indicate a male fetus.
Claim 16
Dependent Claim
19 words
Process of claim 13 wherein the step of determining whether a reaction occurs comprises an immunofluorescence flow cytometry assay.
Claim 17
Dependent Claim
19 words
Process of claim 2 wherein the step of determining whether a reaction occurs comprises an immunofluorescence flow cytometry assay.
Claim 18
Dependent Claim
40 words
Process of claim 2, wherein the step of determining whether a reaction occurs comprises an assay method selected from the group consisting of radioimmuno assay, enzyme-linked antibody binding assay (ELISA), direct or indirect immunofluorescence assay, and immunofluorescence flow cytometry assay.
Claim 19
Dependent Claim
33 words
Monoclonal antibodies which specifically bind to H-Y antigen selected from the group consisting of 12/44, 12/49, and 6/11 produced by hybridoma cell line ATCC HB 9070, ATCC HB 9071 or ATCC HB 9072.
Claim 20
Dependent Claim
33 words
Monoclonal antibodies which specifically bind to H-Y antigen selected from the group consisting of 12/44, 12/49, and 6/11 produced by hybridoma cell line ATCC HB 9070, ATCC HB 9071 or ATCC HB 9072.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-07-13 | 1991-01-12 | 1991-07-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-07-13 | 1995-01-12 | 1995-07-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-07-13 | 1999-01-12 | 1999-07-14 | $7700.00 | Window Closed / Lapsed |