US4076797A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Radioimmunoassay
Assignee: Hoffmann La Roche Inc
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Filing: 1976-09-28
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Granted: 1978-02-28
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Expires: 1996-09-28
Abstract
Abstract A radioimmunoassay for syphilis using Treponema pallidum reiter variant as the antigen in an indirect procedure is disclosed.
Inventors
- 👤 Raymond Vincent Davis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 15:51
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/81
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
129 words
An indirect, solid phase radioimmunoassay for syphilis comprising the steps of (a) fixing Treponema pallidum reiter variant to the inner surface of a test tube, (b) dispensing test samples of human serum diluted with a sorbent containing group specific antibody binders into the test tube in contact with the Treponema pallidum reiter variant and incubating, (c) washing the inner surface of the test tube to remove unreacted elements of the test sample, (d) dispensing 125 I antihuman gamma globulin into the test tube in contact with the antigenic surface and incubating, (e) washing the inner surface of the test tube to remove unreacted 125 I antihuman gamma globulin, (f) measuring the radioactivity in the test tube and comparing to a standard to determine the presence or absence of syphilis.
Claim 2
Dependent Claim
129 words
An indirect, solid phase radioimmunoassay for syphilis comprising the steps of (a) fixing Treponema pallidum reiter variant to the inner surface of a test tube, (b) dispensing test samples of human serum diluted with a sorbent containing group specific antibody binders into the test tube in contact with the Treponema pallidum reiter variant and incubating, (c) washing the inner surface of the test tube to remove unreacted elements of the test sample, (d) dispensing 125 I antihuman gamma globulin into the test tube in contact with the antigenic surface and incubating, (e) washing the inner surface of the test tube to remove unreacted 125 I antihuman gamma globulin, (f) measuring the radioactivity in the test tube and comparing to a standard to determine the presence or absence of syphilis.
Claim 3
Dependent Claim
40 words
The method of claim 1 wherein in step (a) the Treponema pallidum reiter variant is fixed to the inner surface of the test tube on and near the enclosed end thereof by adding acetone, incubating, discarding the acetone and drying.
Claim 4
Dependent Claim
26 words
The method of claim 1 wherein the antihuman gamma globulin is immunospecifically purified by immunoadsorption to human gamma globulin followed by separation therefrom prior to use.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-02-27 | 1981-08-29 | 1982-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-02-27 | 1985-08-29 | 1986-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-02-27 | 1989-08-29 | 1990-02-28 | $7700.00 | Window Closed / Lapsed |