US4191749A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and material for increasing the percentage of mammalian offspring of either sex
Assignee: Individual
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Filing: 1977-10-11
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Granted: 1980-03-04
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Expires: 1997-10-11
Abstract
Abstract Method, apparatus and material are disclosed for increasing the percentage of mammalian offspring of either sex. The method utilizes a male-specific antibody coupled to a solid-phase immunoabsorbent material to effect a separation of sex-determining spermatazoa derived from a semen suspension. The male-specific antibody selectively binds male-determining spermatazoa. The female-determining spermatazoa are not bound and are recovered directly from the male-specific antibody-coated immunoabsorbent material. The male-determining spermatazoa are recovered from the male-specific antibody-coated immunoabsorbent material after altering the condition thereof to inhibit binding. The apparatus includes a vertical surface comprising beads of immunoabsorbent material over which are distributed male-specific antibodies. The materials are two seminal fluids, one having a substantial preponderance of male-determining spermatazoa, and the other having a substantial preponderance of female-determining spermatazoa.
Inventors
- 👤 Bernard J. Bryant
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 09:51
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/52; Y; Y10; Y10S; Y10S424/00; Y10S424/811
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
33 words
As a new composition, a conjugate of activated solid phase immunoabsorbent material having monospecific malespecific antibodies capable of binding male specific sperm distributed over the surface of said immunoabsorbent material and coupled thereto.
Claim 2
Dependent Claim
33 words
As a new composition, a conjugate of activated solid phase immunoabsorbent material having monospecific malespecific antibodies capable of binding male specific sperm distributed over the surface of said immunoabsorbent material and coupled thereto.
Claim 3
Dependent Claim
23 words
The composition of claim 1 in which said activated solid phase immunoabsorbent material is in the form of a plurality of discrete beads.
Claim 4
Dependent Claim
19 words
The composition claimed in claim 2 in which said plurality of beads comprise polysaccharide beads disposed in a column.
Claim 5
Dependent Claim
31 words
The composition of claim 1 wherein said immunoabsorbent material, has been activated to render it capable of accepting protein molecules by covalent bonding, by the treatment thereof with a cyanogen halide.
Claim 6
Dependent Claim
29 words
The composition of claim 3 wherein said polysaccharide beads have been activated to render them capable of accepting protein molecules by covalent bonding by treatment with a cyanogen halide.
Claim 7
Dependent Claim
12 words
The composition of claim 5 wherein the cyanogen halide is cyanogen bromide.
Claim 8
Dependent Claim
74 words
A process for separating male and female determining sperm from native semen which comprises: (a) coupling monospecific male specific antibodies to a solid phase immunoabsorbent substrate to form a conjugate, (b) loading a separation column with the monospecific male specific conjugate, (c) passing semen containing male and female determining sperm through the column, (d) separating out the female determining sperm from the column, and (e) separating out the male determining sperm from the column.
Claim 9
Dependent Claim
74 words
A process for separating male and female determining sperm from native semen which comprises: (a) coupling monospecific male specific antibodies to a solid phase immunoabsorbent substrate to form a conjugate, (b) loading a separation column with the monospecific male specific conjugate, (c) passing semen containing male and female determining sperm through the column, (d) separating out the female determining sperm from the column, and (e) separating out the male determining sperm from the column.
Claim 10
Dependent Claim
29 words
The process of claim 7 wherein the step of separating out the male determining sperm comprises passing a composition consisting essentially of monospecific male specific antibody through the column.
Claim 11
Dependent Claim
38 words
The process of claim 7 wherein the coupling step (a) comprises: activating the solid phase immunoabsorbent material to render it capable of accepting a protein by covalent bonding, and adding a solution of monospecific male specific antibody thereto.
Claim 12
Dependent Claim
30 words
The process as claimed in claim 7 in which said body of semen contains the naturally-occurring ratio of male-determining spermatazoa to female-determining spermatoazoa prior to said separation of male-determining spermatazoa.
Claim 13
Dependent Claim
40 words
The process as claimed in claim 8 further comprising: incorporating said male-determining spermatazoa into a fluid medium suitable for use in artificial insemination; and artificially inseminating a female of the same species with said fluid medium containing said male-determining spermatazoa.
Claim 14
Dependent Claim
40 words
The process as claimed in claim 7 further comprising: incorporating said female-determining spermatazoa into a fluid medium suitable for use in artifical insemination; and artificially inseminating a female of the same species with said fluid medium containing said female-determining spermatazoa.
Claim 15
Independent Claim
44 words
The method of increasing the percentage of mammalian offspring of either sex, comprising the step of selectively separating male-determining spermatazoa by contacting a body of seman with a male specific antibody capable of binding male specific sperm, said antibody being coupled to immunoabsorbent material.
Claim 16
Independent Claim
44 words
The method of increasing the percentage of mammalian offspring of either sex, comprising the step of selectively separating male-determining spermatazoa by contacting a body of seman with a male specific antibody capable of binding male specific sperm, said antibody being coupled to immunoabsorbent material.
Claim 17
Dependent Claim
20 words
The method as claimed in claim 13, further comprising the step of altering the immunoabsorbent material to release male-determining spermatazoa.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-04 | 1983-09-03 | 1984-03-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-04 | 1987-09-03 | 1988-03-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-04 | 1991-09-03 | 1992-03-04 | $7700.00 | Window Closed / Lapsed |