Patent US535722A successfully refreshed from Google Patents.
Patent US4650751A successfully refreshed from Google Patents.
US4650751A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Protected binding assay avoiding non-specific protein interference
Assignee: Bayer Corp
•
Filing: 1983-04-29
•
Granted: 1987-03-17
•
Expires: 2003-04-29
Abstract
Abstract A specific binding enzyme-resistant ligand assay test material, which material comprises (a) a solid phase incorporated with one partner of a specific binding pair comprising said ligand or a binding analog thereof and a specific binding protein therefor; (b) a conjugate comprising the other partner of said specific binding pair incorporated with a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme. Also a specific binding method of assaying for an enzyme-resistant ligand in a sample, which method uses the above test material and which results in a reduction in interference caused by non-specific protein.
Inventors
- 👤 Richard C. Siegel
- 👤 Christina S. Marx
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:09
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/962; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/825; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 52
Claim 1
Independent Claim
100 words
A specific binding assay test composition for determination of an enzyme-resistant ligand in a sample wherein non-specific protein interference is avoided, which test composition comprises: (a) a solid phase incorporated with one partner of a specific binding pair comprising (1) said ligand or a binding analog of said ligand and (2) a specific binding protein for said ligand; (b) a conjugate comprising the other partner of said specific binding pair which is covalently bound to a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme.
Claim 2
Dependent Claim
100 words
A specific binding assay test composition for determination of an enzyme-resistant ligand in a sample wherein non-specific protein interference is avoided, which test composition comprises: (a) a solid phase incorporated with one partner of a specific binding pair comprising (1) said ligand or a binding analog of said ligand and (2) a specific binding protein for said ligand; (b) a conjugate comprising the other partner of said specific binding pair which is covalently bound to a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme.
Claim 3
Dependent Claim
25 words
The composition of claim 1 wherein said solid phase incorporated with a specific binding partner comprises a particle having a specific binding protein surface moiety.
Claim 4
Dependent Claim
25 words
The composition of claim 1 wherein said solid phase incorporated with a specific binding partner comprises a substrate particle bound to a specific binding protein.
Claim 5
Dependent Claim
23 words
The composition of claim 3 wherein said substrate particle is selected from the group consisting of eucaryotic cells, procaryotic cells and synthetic particles.
Claim 6
Dependent Claim
11 words
The composition of claim 4 wherein said synthetic particles are latex.
Claim 7
Dependent Claim
13 words
The composition of claim 1 wherein said specific binding protein is an antibody.
Claim 8
Dependent Claim
12 words
The composition of claim 6 wherein said antibody is specific to triiodothyronine.
Claim 9
Dependent Claim
12 words
The composition of claim 6 wherein said antibody is specific to thyroxine.
Claim 10
Dependent Claim
19 words
The composition of claim 6 wherein said antibody is present in a concentration of at least about 0.04 percent.
Claim 11
Dependent Claim
21 words
The composition of claim 9 wherein said antibody is present in a concentration of from about 0.04 to about 0.07 percent.
Claim 12
Dependent Claim
27 words
The composition of claim 1 wherein said conjugate of said enzyme-resistant ligand comprises a ligand or specific binding analog thereof incorporated with a high molecular weight carrier.
Claim 13
Dependent Claim
24 words
The composition of claim 11 wherein said conjugate comprises a ligand or specific binding analog thereof covalently bound with a high molecular weight carrier.
Claim 14
Dependent Claim
14 words
The composition of claim 12 wherein said high molecular weight carrier is a polymer.
Claim 15
Dependent Claim
10 words
The composition of claim 13 wherein said polymer is dextran.
Claim 16
Dependent Claim
16 words
The composition of claim 13 wherein said polymer is the cross-linking product of epichlorohydrin and sucrose.
Claim 17
Dependent Claim
10 words
The composition of claim 11 wherein said ligand is triiodothyronine.
Claim 18
Dependent Claim
10 words
The composition of claim 11 wherein said ligand is thyroxine.
Claim 19
Dependent Claim
11 words
The composition of claim 1 wherein said protein-inactivating enzyme is trypsin.
Claim 20
Dependent Claim
11 words
The composition of claim 1 wherein said protein-inactivating enzyme is pepsin.
Claim 21
Dependent Claim
23 words
The composition of claim 1 wherein said protein-inactivating enzyme is present in a concentration of not more than about 4.0 milligrams per milliliter.
Claim 22
Dependent Claim
24 words
The composition of claim 20 wherein said protein-inactivating enzyme is present in a concentration of from about 2.0 to about 4.0 milligrams per milliliter.
Claim 23
Dependent Claim
43 words
A specific binding assay test composition for thyroxine or triiodothyronine, which composition comprises: (a) particle-associated thyroxine or triiodothyronine antibody; (b) trypsin; and (c) a conjugate comprising thyroxine or triiodothyronine covalently bound with a high molecular weight polymer whereby non-specific protein interference is avoided.
Claim 24
Dependent Claim
43 words
A specific binding assay test composition for thyroxine or triiodothyronine, which composition comprises: (a) particle-associated thyroxine or triiodothyronine antibody; (b) trypsin; and (c) a conjugate comprising thyroxine or triiodothyronine covalently bound with a high molecular weight polymer whereby non-specific protein interference is avoided.
Claim 25
Dependent Claim
66 words
The specific binding assay composition of claim 22 wherein (a) said particle-associated thyroxine or triiodothyronine antibody is present in a concentration of from about 0.04 to about 0.07 percent; (b) said trypsin is present in a concentration of from about 2.0 to about 4.0 milligrams per milliliter; and (c) said conjugate is present in a concentration of from about 0.22 to about 0.88 micrograms per milliliter.
Claim 26
Dependent Claim
121 words
A specific binding method of assaying for an enzyme-resistant ligand in a sample wherein non-specific protein interference is avoided, which method consists essentially of the steps of: (i) combining said sample in a reaction mixture with: (a) a solid phase incorporated with one partner of a specific binding pair comprising (1) said ligand or a binding analog of said ligand and (2) a specific binding protein for said ligand; (b) a conjugate comprising the other partner of said specific binding pair which is covalently bound to a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme; and (ii) detecting any resultant binding in said reaction mixture.
Claim 27
Dependent Claim
121 words
A specific binding method of assaying for an enzyme-resistant ligand in a sample wherein non-specific protein interference is avoided, which method consists essentially of the steps of: (i) combining said sample in a reaction mixture with: (a) a solid phase incorporated with one partner of a specific binding pair comprising (1) said ligand or a binding analog of said ligand and (2) a specific binding protein for said ligand; (b) a conjugate comprising the other partner of said specific binding pair which is covalently bound to a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme; and (ii) detecting any resultant binding in said reaction mixture.
Claim 28
Dependent Claim
21 words
The method of claim 24 wherein said particle-associated specific binding protein comprises a particle having a specific binding protein surface moiety.
Claim 29
Dependent Claim
21 words
The method of claim 24 wherein said particle-associated specific binding protein comprises a substrate particle bound to a specific binding protein.
Claim 30
Dependent Claim
23 words
The method of claim 24 wherein said substrate particle is selected from the group consisting of eucaryotic cells, procaryotic cells and synthetic particles.
Claim 31
Dependent Claim
12 words
The method of claim 27 wherein said synthetic particles are latex particles.
Claim 32
Dependent Claim
13 words
The method of claim 24 wherein said specific binding protein is an antibody.
Claim 33
Dependent Claim
12 words
The method of claim 29 wherein said antibody is specific to triiodothyronine.
Claim 34
Dependent Claim
12 words
The method of claim 29 wherein said antibody is specific to thyroxine.
Claim 35
Dependent Claim
19 words
The method of claim 29 wherein said antibody is present in a concentration of at least about 0.04 percent.
Claim 36
Dependent Claim
22 words
The method of claim 32 wherein said antibody is present in a concentration of from about 0.04 percent to about 0.07 percent.
Claim 37
Dependent Claim
11 words
The method of claim 24 wherein said protein-inactivating enzyme is trypsin.
Claim 38
Dependent Claim
11 words
The method of claim 24 wherein said protein-inactivating enzyme is pepsin.
Claim 39
Dependent Claim
24 words
The method of claim 24 wherein said active protein-inactivating enzyme is present in a concentration of not more than about 4.0 milligrams per milliliter.
Claim 40
Dependent Claim
24 words
The method of claim 36 wherein said protein-inactivating enzyme is present in a concentration of from about 2.0 to about 4.0 milligrams per milliliter.
Claim 41
Dependent Claim
24 words
The method of claim 24 wherein said conjugate comprises an enzyme-resistant ligand or specific binding analog thereof incorporated with a high molecular weight carrier.
Claim 42
Dependent Claim
24 words
The method of claim 38 wherein said conjugate comprises a ligand or specific binding analog thereof covalently bound with a high molecular weight carrier.
Claim 43
Dependent Claim
14 words
The method of claim 39 wherein said high molecular weight carrier is a polymer.
Claim 44
Dependent Claim
10 words
The method of claim 40 wherein said polymer is dextran.
Claim 45
Dependent Claim
16 words
The method of claim 40 wherein said polymer is the cross-linking product of epichlorohydrin and sucrose.
Claim 46
Dependent Claim
10 words
The method of claim 38 wherein said ligand is triiodothyronine.
Claim 47
Dependent Claim
10 words
The method of claim 38 wherein said ligand is thyroxine.
Claim 48
Dependent Claim
66 words
A specific binding method of assaying for thyroxine or triiodothyronine in a sample wherein non-specific protein interference is avoided, which method consists essentially of (i) combining said sample in a reaction mixture with: (a) particle-associated thyroxine or triiodothyronine antibody; (b) a conjugate comprising thyroxine or triidothyronine covalently bound with a high molecular weight polymer; (c) trypsin; and (ii) detecting any resultant binding in said reaction mixture.
Claim 49
Dependent Claim
66 words
A specific binding method of assaying for thyroxine or triiodothyronine in a sample wherein non-specific protein interference is avoided, which method consists essentially of (i) combining said sample in a reaction mixture with: (a) particle-associated thyroxine or triiodothyronine antibody; (b) a conjugate comprising thyroxine or triidothyronine covalently bound with a high molecular weight polymer; (c) trypsin; and (ii) detecting any resultant binding in said reaction mixture.
Claim 50
Dependent Claim
65 words
The specific binding assay of claim 45 wherein: (a) said particle-associated thyroxine or triiodothyronine antibody is present in a concentration of from about 0.04 to about 0.07 percent; (b) said trypsin is present in a concentration of from about 2.0 to about 4.0 milligrams per milliliter; and (c) said conjugate is present in a concentration of from about 0.22 to about 0.88 micrograms per milliliter.
Claim 51
Dependent Claim
112 words
A test kit for use in determining a ligand in a sample by a specific binding assay method, which kit comprises the packaged combination of one or more components incorporated with: (a) a solid phase incorporated with one partner of a specific binding pair comprising (1) said ligand or a binding analog of said ligand and (2) a specific binding protein for said ligand; (b) a conjugate comprising the other partner of said specific binding pair which is covalently bound to a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme whereby non-specific protein interference is avoided.
Claim 52
Dependent Claim
112 words
A test kit for use in determining a ligand in a sample by a specific binding assay method, which kit comprises the packaged combination of one or more components incorporated with: (a) a solid phase incorporated with one partner of a specific binding pair comprising (1) said ligand or a binding analog of said ligand and (2) a specific binding protein for said ligand; (b) a conjugate comprising the other partner of said specific binding pair which is covalently bound to a substance which protects the specific binding protein of said pair from enzyme inactivation when bound with its partner; and (c) an active protein-inactivating enzyme whereby non-specific protein interference is avoided.
References Cited (Backward Citations)
US4032626A
Cited
US4225576A
Cited
US4362531A
Cited
US4455381A
Cited
US4427781A
Cited
US4578350A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-16 | 1990-09-15 | 1991-03-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-16 | 1994-09-15 | 1995-03-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-16 | 1998-09-15 | 1999-03-17 | $7700.00 | Window Closed / Lapsed |