US4362531A
Expired / Lapsed
US
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Agglutination immunoassays carried out with agent to reduce non-specific interferences
Assignee: Bayer Corp
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Filing: 1981-04-14
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Granted: 1982-12-07
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Expires: 2001-04-14
Abstract
Abstract In particle agglutination immunoassays for an analyte (e.g. an antigen or antibody) in a liquid sample (e.g. human serum), interferences arise due to non-specific protein-protein interactions and the like. These interferences are reduced or overcome by including in the assay mixture a chaotropic or chaotropic-like agent, in a carefully controlled amount. Such agents include guanidine, guanidinium hydrochloride or thiocyanate, sodium or ammonium thiocyanate, and urea, sodium chloride and ethylenediamine tetraacetic acid.
Inventors
- 👤 Floris de Steenwinkel
- 👤 Daniel Collet-Cassart
- 👤 Pierre L. Masson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 12:14
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; Y; Y10; Y10S; Y10S436/00; Y10S436/825
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
138 words
A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination or the particles occurs to an extent dependent on the amount of analyte present, and determining said extent and thereby the amount of analyte present, wherein the mixture also includes one or more agents to reduce non-specific protein interaction interferences, the amount of said agent(s) being sufficient to reduce the effect on agglutination of said interferences, wherein said agents comprise at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 2
Independent Claim
138 words
A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination or the particles occurs to an extent dependent on the amount of analyte present, and determining said extent and thereby the amount of analyte present, wherein the mixture also includes one or more agents to reduce non-specific protein interaction interferences, the amount of said agent(s) being sufficient to reduce the effect on agglutination of said interferences, wherein said agents comprise at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 3
Dependent Claim
43 words
A method according to claim 1, wherein the agent is selected from the group consisting of sodium chloride, ethylene diamine tetra-acetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 4
Dependent Claim
20 words
A method according to claim 1, wherein the mixture also contains a macromolecular substance to enhance the specific agglutination reaction.
Claim 5
Dependent Claim
43 words
A method according to claim 1, wherein the analyte is selected from antigens and haptens, and wherein the mixture also contains the F(ab') 2 fragments of an immunoglobulin specific to said analyte, but is free from the whole immunoglobulin and F(c) fragments thereof.
Claim 6
Dependent Claim
28 words
A method according to claim 1, wherein the mixture contains two different particles each comprising a protein and a reagent, the particles differing in respect of the reagent.
Claim 7
Dependent Claim
19 words
A method according to claim 1, wherein said protein of which the particles are comprised also constitutes said reagent.
Claim 8
Dependent Claim
21 words
A method according to claim 1, wherein the agent is selected from guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate and ammonium thiocyanate.
Claim 9
Dependent Claim
23 words
A method according to claim 7, wherein the agent is present in the mixture in the amount from 0.5 M to 2 M.
Claim 10
Dependent Claim
28 words
A method according to claim 1, wherein the mixture contains two different particles each comprising a protein and a reagent, the particles differing in respect of their size.
Claim 11
Dependent Claim
22 words
A method according to claim 9, wherein said protein of which each particle is comprised also constitutes said reagent of that particle.
Claim 12
Dependent Claim
18 words
A method according to claim 1, wherein the said extent of agglutination is determined by counting unagglutinated particles.
Claim 13
Dependent Claim
46 words
A method according to claim 11, wherein the agent is selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, and lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 14
Independent Claim
183 words
A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination of the particles occurs to an extent dependent on the amount of analyte present, and determining the said extent and thereby the amount of analyte present, wherein (a) as a preliminary step, solutions of the same analyte as is under assay are mixed with varying amounts of an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide and said particles, and the solutions assayed to determine the optimum range of said agent to reduce non-specific protein interaction interferences whilst retaining the desired immunospecific reaction upon which the assay is based; and then (b) assaying the analyte in said liquid sample in the presence of said agent in an amount within said range.
Claim 15
Independent Claim
183 words
A method of immunoassay for an analyte in a liquid sample, which comprises forming a mixture of the liquid sample with finely divided particles comprising a protein and a reagent, whereby specific agglutination of the particles occurs to an extent dependent on the amount of analyte present, and determining the said extent and thereby the amount of analyte present, wherein (a) as a preliminary step, solutions of the same analyte as is under assay are mixed with varying amounts of an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide and said particles, and the solutions assayed to determine the optimum range of said agent to reduce non-specific protein interaction interferences whilst retaining the desired immunospecific reaction upon which the assay is based; and then (b) assaying the analyte in said liquid sample in the presence of said agent in an amount within said range.
Claim 16
Dependent Claim
88 words
A particle agglutination specific binding assay composition for the non-specific protein interaction interference-free determination of an analyte in a liquid sample, which composition comprises: a particle to which is bound protein and a specific binding partner for said analyte; and an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 17
Dependent Claim
88 words
A particle agglutination specific binding assay composition for the non-specific protein interaction interference-free determination of an analyte in a liquid sample, which composition comprises: a particle to which is bound protein and a specific binding partner for said analyte; and an agent comprising at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 18
Dependent Claim
51 words
The particle agglutination specific binding assay composition of claim 14, wherein said agent comprises at least one compound selected from the group consisting of sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide.
Claim 19
Dependent Claim
33 words
The particle agglutination specific binding assay composition of claim 14, wherein said agent comprises at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate and ammonium thiocyanate.
Claim 20
Dependent Claim
16 words
The particle agglutination specific binding assay composition of claim 16, wherein said agent comprises sodium thiocyanate.
Claim 21
Dependent Claim
105 words
A particle agglutination specific binding assay method for the non-specific protein interference-free determination of an analyte in a liquid sample, which method comprises: contacting said liquid sample with a composition which comprises a particle to which is bound protein and a specific binding partner for said analyte and an agent which comprises at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide; and, thereafter, observing any particle agglutination which occurs.
Claim 22
Dependent Claim
105 words
A particle agglutination specific binding assay method for the non-specific protein interference-free determination of an analyte in a liquid sample, which method comprises: contacting said liquid sample with a composition which comprises a particle to which is bound protein and a specific binding partner for said analyte and an agent which comprises at least one compound selected from the group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate, ammonium thiocyanate, sodium chloride, ethylene diamine tetraacetic acid, lithium nitrate, lithium chlorate, lithium isocyanate, lithium bromide, sodium bromide, potassium bromide, potassium thiocyanate, calcium chloride, lithium chloride and lithium iodide; and, thereafter, observing any particle agglutination which occurs.
Claim 23
Dependent Claim
42 words
The method of claim 18 wherein the step of contacting comprises contacting said liquid sample with a composition wherein said chaotropic agent includes at least one compound selected from one group consisting of guanidinium hydrochloride, guanidinium thiocyanate, sodium thiocyanate and ammonium thiocyanate.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-12-06 | 1986-06-07 | 1986-12-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-12-06 | 1990-06-07 | 1990-12-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-12-06 | 1994-06-07 | 1994-12-07 | $7700.00 | Window Closed / Lapsed |