US4292038A
Expired / Lapsed
US
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Formamide-containing latex agglutinating reagent for immunoassay
Assignee: Takeda Pharmaceutical Co Ltd
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Filing: 1980-01-29
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Granted: 1981-09-29
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Expires: 2000-01-29
Abstract
Abstract By using a reagent for latex agglutination reaction which contains one or more members selected from the group consisting of a compound of the formula ##STR1## wherein R 1 is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide, the non-specific agglutination can be remarkably prevented and thus greatly improving the reliability of the latex agglutination test results.
Inventors
- 👤 Koichi Kondo
- 👤 Isamu Yoshida
- 👤 Takashi Kobayashi
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 06:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/814; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/825
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
93 words
In an immunoassay comprising incubating a test sample with particles sensitized with an antigen or antibody and observing any resulting agglutination, the improvement wherein the reaction is conducted in the presence of one or more members selected from the group consisting of a compound of the formula ##STR3## wherein R 1 is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide.
Claim 2
Dependent Claim
93 words
In an immunoassay comprising incubating a test sample with particles sensitized with an antigen or antibody and observing any resulting agglutination, the improvement wherein the reaction is conducted in the presence of one or more members selected from the group consisting of a compound of the formula ##STR3## wherein R 1 is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide.
Claim 3
Dependent Claim
23 words
A method according to claim 1, wherein the concentration of said member is about 1 to 13% relative to the whole reaction system.
Claim 4
Dependent Claim
17 words
A method according to claim 2, wherein the concentration of said member is about 1 to 7.
Claim 5
Dependent Claim
121 words
A diluent for a test sample in an immunoassay which involves incubating the test sample together with latex particles sensitized with an antigen or antibody and observing any resulting agglutination, said diluent containing about 4% to 52% of one or more members selected from the group consisting of a compound of the formula ##STR4## wherein R is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide, in a buffer liquid having a pH range of about 6 to 9 and an electrolyte concentration of about 0.05 to 0.3 M.
Claim 6
Dependent Claim
121 words
A diluent for a test sample in an immunoassay which involves incubating the test sample together with latex particles sensitized with an antigen or antibody and observing any resulting agglutination, said diluent containing about 4% to 52% of one or more members selected from the group consisting of a compound of the formula ##STR4## wherein R is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide, in a buffer liquid having a pH range of about 6 to 9 and an electrolyte concentration of about 0.05 to 0.3 M.
Claim 7
Dependent Claim
15 words
A diluent according to claim 4, which contains both urea and N,N-dimethylformamide as said member.
Claim 8
Dependent Claim
85 words
A diagnostic reagent which contains about 2% to 52% of one or more members selected from the group consisting of a compound of the formula ##STR5## wherein R 1 is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide, and about 0.4% to 1.4% of latex particles sensitized with an antigen or antibody.
Claim 9
Dependent Claim
85 words
A diagnostic reagent which contains about 2% to 52% of one or more members selected from the group consisting of a compound of the formula ##STR5## wherein R 1 is a hydrogen atom, a lower alkyl group or an amino group which may be substituted with a lower alkyl group and R 2 and R 3 are each a hydrogen atom or a lower alkyl group, and a di-lower alkyl sulfoxide, and about 0.4% to 1.4% of latex particles sensitized with an antigen or antibody.
Claim 10
Dependent Claim
14 words
A diagnostic reagent according to claim 6, wherein the latex particles are of polystyrene.
Claim 11
Dependent Claim
23 words
A diagnostic reagent according to claim 6, wherein said member is N,N-dimethylformamide and the latex particles are ones sensitized with anti-C-reactive protein antibody.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-09-28 | 1985-03-30 | 1985-09-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-09-28 | 1989-03-30 | 1989-09-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-09-28 | 1993-03-30 | 1993-09-29 | $7700.00 | Window Closed / Lapsed |