Patent US2442351A successfully refreshed from Google Patents.
US4046723A
Expired / Lapsed
US
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Azide bonding of a protein to a latex
Assignee: Dow Chemical Co
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Filing: 1976-04-22
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Granted: 1977-09-06
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Expires: 1996-04-22
Abstract
Abstract A method of coupling a latex having a surface carboxylic amide group to a protein by an amide bond for producing a composition useful in immunological testing for antibodies or antigen.
Inventors
- 👤 Linneaus C. Dorman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:48
CPC Classifications
C; C08; C08G; C08G81/00; C08G81/02; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/823; Y10S528/00; Y10S528/92
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
77 words
A method for coupling a latex having surface carboxylic amide groups to a protein having free amino groups which comprises hydrazinolysing the carboxylic amide group on the latex to a carboxylic hydrazide group by treatment with hydrazine, diazotizing the carboxylic hydrazide group on the latex with a nitrite in the presence of an acid to form a carboxylic azide group, and coupling the carboxylic azide group of the latex with a free amino group on the protein.
Claim 2
Independent Claim
77 words
A method for coupling a latex having surface carboxylic amide groups to a protein having free amino groups which comprises hydrazinolysing the carboxylic amide group on the latex to a carboxylic hydrazide group by treatment with hydrazine, diazotizing the carboxylic hydrazide group on the latex with a nitrite in the presence of an acid to form a carboxylic azide group, and coupling the carboxylic azide group of the latex with a free amino group on the protein.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the latex is a monodispersed latex.
Claim 4
Dependent Claim
14 words
The method of claim 2 wherein the latex is a monodispersed styrene acrylamide latex.
Claim 5
Dependent Claim
12 words
The method of claim 3 wherein the protein is human chorionic gonadotropin.
Claim 6
Dependent Claim
20 words
The method of claim 1 wherein the nitrite is sodium nitrite and the acid is a strong non-oxidizing mineral acid.
Claim 7
Independent Claim
89 words
A method for coupling monodispersed styrene acrylamide latex to human chorionic gonadotropin comprising hydrazinolysing the carboxylic amide group on the latex to a carboxylic hydrazide by treatment with hydrazine at a temperature of 40°-60° C., dialyzing the latex for a time sufficient to remove unreacted hydrazine, diazotizing the carboxylic hydrazide group formed on the latex with sodium nitrite and hydrochloric acid, and coupling the human chorionic gonadotropin to the latex at a pH of 7-9.5 at a temperature of 2°-5° C. for a period of at least 24 hours.
Claim 8
Independent Claim
89 words
A method for coupling monodispersed styrene acrylamide latex to human chorionic gonadotropin comprising hydrazinolysing the carboxylic amide group on the latex to a carboxylic hydrazide by treatment with hydrazine at a temperature of 40°-60° C., dialyzing the latex for a time sufficient to remove unreacted hydrazine, diazotizing the carboxylic hydrazide group formed on the latex with sodium nitrite and hydrochloric acid, and coupling the human chorionic gonadotropin to the latex at a pH of 7-9.5 at a temperature of 2°-5° C. for a period of at least 24 hours.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-09-05 | 1981-03-07 | 1981-09-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-09-05 | 1985-03-07 | 1985-09-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-09-05 | 1989-03-07 | 1989-09-06 | $7700.00 | Window Closed / Lapsed |