US4032626A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagent and method for tbg assay
Assignee: Corning Glass Works
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Filing: 1976-06-30
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Granted: 1977-06-28
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Expires: 1996-06-30
Abstract
Abstract The concentration of thyroxine binding globulin (TBG) in a fluid sample can be determined via radioassay technique with an immunochemical composite comprising anti-TBG antibodies immobilized on a water-insoluble carrier. The composite is incubated with the sample in a quantity sufficient to assure complexation of substantially all TBG in the sample to the antibodies of the composite. The quantity of TBG complexed is determined with radiolabeled thyroxine (T 4 ) which complexes with at least a portion of the complexed TBG. By separating the labeled composite from the remaining labeled T 4 , and counting the bound (or unbound) T 4 , the concentration of TBG can be determined from a standard curve.
Inventors
- 👤 Frank B. Ward
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 10:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/81; Y10S436/825
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
148 words
A method of determining the concentration of thyroxine binding globulin is a fluid sample, the method comprising the steps of: (A) incubating the sample with (i) a composite comprising antibodies to thyroxine binding globulin immobilized on an insoluble carrier material, the incubation being under conditions, and with composite quantity sufficient to complex substantially all thyroxine binding globulin in the sample onto the antibodies of the composite; and (ii) a quantity of radiolabeled thyroxine in a quantity sufficient to complex with at least a portion of the thyroxine binding globulin complexed to the antibodies of the composite; (B) separating the composite and materials complexed thereto from unbound radiolabeled thyroxine; (C) counting the radioactivity of the separated composite or the remaining radiolabeled thyroxine; and (D) correlating the count of step (C) with a standard curve relating concentrations of thyroxine binding globulin to counts of the type determined by step (C).
Claim 2
Independent Claim
148 words
A method of determining the concentration of thyroxine binding globulin is a fluid sample, the method comprising the steps of: (A) incubating the sample with (i) a composite comprising antibodies to thyroxine binding globulin immobilized on an insoluble carrier material, the incubation being under conditions, and with composite quantity sufficient to complex substantially all thyroxine binding globulin in the sample onto the antibodies of the composite; and (ii) a quantity of radiolabeled thyroxine in a quantity sufficient to complex with at least a portion of the thyroxine binding globulin complexed to the antibodies of the composite; (B) separating the composite and materials complexed thereto from unbound radiolabeled thyroxine; (C) counting the radioactivity of the separated composite or the remaining radiolabeled thyroxine; and (D) correlating the count of step (C) with a standard curve relating concentrations of thyroxine binding globulin to counts of the type determined by step (C).
Claim 3
Dependent Claim
21 words
The method, as claimed in claim 1, wherein the incubation of step (A)(ii) is subsequent to the incubation of step (A)(i).
Claim 4
Dependent Claim
22 words
The method of claim 1 wherein the composite comprises antibodies to thyroxine binding globulin fixed on the surfaces of an inorganic carrier.
Claim 5
Dependent Claim
24 words
The method of claim 3 wherein the inorganic carrier comprises glass particles which, with the antibodies fixed thereon, are suspendable in the incubation medium.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the radiolabeled thyroxine is labeled with I 125 .
Claim 7
Dependent Claim
19 words
The method of claim 1 wherein the total incubation time of step A is less than about 60 minutes.
Claim 8
Dependent Claim
37 words
A reagent for determining the concentration of thyroxine binding globulin in a fluid sample, the reagent being an immunochemical composite comprising antibodies to thyroxine binding globulin attached to the surfaces of an essentially water insoluble carrier material.
Claim 9
Dependent Claim
37 words
A reagent for determining the concentration of thyroxine binding globulin in a fluid sample, the reagent being an immunochemical composite comprising antibodies to thyroxine binding globulin attached to the surfaces of an essentially water insoluble carrier material.
Claim 10
Dependent Claim
11 words
The reagent of claim 7 wherein the carrier material is inorganic.
Claim 11
Dependent Claim
13 words
The reagent of claim 8 wherein the carrier consists of silanized glass particles.
Claim 12
Dependent Claim
27 words
The reagent of claim 9 wherein the particles are suspendable in an aqueous solution and have an average particle size ranging from about 0.01 to 10 microns.
Claim 13
Dependent Claim
11 words
The reagent of claim 10 wherein the glass particles are porous.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-06-27 | 1980-12-27 | 1981-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-06-27 | 1984-12-27 | 1985-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-06-27 | 1988-12-27 | 1989-06-28 | $7700.00 | Window Closed / Lapsed |