US4639425A
Expired / Lapsed
US
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Heterogeneous immunoassay and reagent therefore
Assignee: Roche Diagnostics GmbH
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Filing: 1984-07-17
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Granted: 1987-01-27
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Expires: 2004-07-17
Abstract
Abstract The invention is an improvement in a heterogeneous immunoassay, and reagent therefore, for the determination of a substance in a blood serum or plasma sample, such as a parnter of an immune reaction, in which one of the reaction partners is adsorbed on a solid carrier, the improvement comprises adding a detergent to the incubation medium of the reaction, in an amount of 0.0001 to 0.01% (w/v) to reduce interferences from the serum or plasma.
Inventors
- 👤 Manfred Baier
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 13:18
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/825; Y10S436/826
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
75 words
In a heterogeneous immunoassay for the determination of a partner of an immune reaction in a blood serum or plasma sample in which one of the reaction partners is adsorbed on a solid carrier, the improvement comprising adding a detergent in such an amount to the incubation medium of the immune reaction between the soluble partner or partners and the partner adsorbed on the solid carrier that its concentration is from 0.0001 to 0.01% (w/v).
Claim 2
Dependent Claim
75 words
In a heterogeneous immunoassay for the determination of a partner of an immune reaction in a blood serum or plasma sample in which one of the reaction partners is adsorbed on a solid carrier, the improvement comprising adding a detergent in such an amount to the incubation medium of the immune reaction between the soluble partner or partners and the partner adsorbed on the solid carrier that its concentration is from 0.0001 to 0.01% (w/v).
Claim 3
Dependent Claim
14 words
The immunoassay of claim 1 wherein the detergent is a non-ionic or anionic detergent.
Claim 4
Dependent Claim
44 words
The immunoassay of claim 1, wherein the detergent used is a polyoxyethylenesorbitan monolaureate, a polyoxyethylene-sorbitan monooleate, an alkyl-polyether-alcohol mixture, a polyoxyethylene lauryl ether, polyoxyethylene octylphenol ether, a polyethyleneoxide-alkyl ether adduct, a C16 or C18 fatty alcohol with 10 oxyethylene units or sodium dodecyl sulphate.
Claim 5
Dependent Claim
92 words
In a competitive heterogeneous immunoassay for the determination of a substance in a blood serum or plasma sample of the type in which a known amount of a tagged substance is added to the sample solution whereupon the substance to be determined and the tagged substance compete for the binding points of a receptor present in insufficient amount, the receptor being adsorbed on a solid carrier, the improvement comprising adding, a detergent in such an amount to the incubation medium of the immune reaction that its concentration is 0.0001 to 0.01% (w/v).
Claim 6
Dependent Claim
92 words
In a competitive heterogeneous immunoassay for the determination of a substance in a blood serum or plasma sample of the type in which a known amount of a tagged substance is added to the sample solution whereupon the substance to be determined and the tagged substance compete for the binding points of a receptor present in insufficient amount, the receptor being adsorbed on a solid carrier, the improvement comprising adding, a detergent in such an amount to the incubation medium of the immune reaction that its concentration is 0.0001 to 0.01% (w/v).
Claim 7
Dependent Claim
82 words
In a heterogeneous sandwich immunoassay for the determination of a bivalent or multivalent substance in a blood serum or plasma sample, of the type in which the substance is brought into contact with a tagged receptor and with a receptor adsorbed on a solid carrier, the improvement comprising adding a detergent in such an amount to the incubation medium of the immune reaction between the acceptor and the receptor adsorbed on a solid carrier that its concentration is 0.0001 to 0.01% (w/v).
Claim 8
Dependent Claim
82 words
In a heterogeneous sandwich immunoassay for the determination of a bivalent or multivalent substance in a blood serum or plasma sample, of the type in which the substance is brought into contact with a tagged receptor and with a receptor adsorbed on a solid carrier, the improvement comprising adding a detergent in such an amount to the incubation medium of the immune reaction between the acceptor and the receptor adsorbed on a solid carrier that its concentration is 0.0001 to 0.01% (w/v).
Claim 9
Dependent Claim
52 words
In a reagent for carrying out an immune reaction between a reaction partner adsorbed on a solid carrier and a soluble partner or partners in a blood serum or plasma sample the improvement comprising a detergent in such an amount that it is present in a concentration of 0.0001 to 0.01% (w/v).
Claim 10
Dependent Claim
52 words
In a reagent for carrying out an immune reaction between a reaction partner adsorbed on a solid carrier and a soluble partner or partners in a blood serum or plasma sample the improvement comprising a detergent in such an amount that it is present in a concentration of 0.0001 to 0.01% (w/v).
Claim 11
Dependent Claim
15 words
The reagent of a claim 6, wherein the detergent is a non-ionic or anionic detergent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-01-26 | 1990-07-28 | 1991-01-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-01-26 | 1994-07-28 | 1995-01-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-01-26 | 1998-07-28 | 1999-01-27 | $7700.00 | Window Closed / Lapsed |