Indirect-photometric chromatography done in and with a variable capacity weakly basic or acidic ion exchange column

Claim 1

In the method for the chromatographic analysis of transparent sample ions in a solution of photometrically monitorable liquid effluent wherein: sample is contacted with a flow-through ion exchange medium, and an elution through said ion exchange medium is performed using a liquid eluent which contains photometrically detectable monitor/displacing ions that effectually displace sample ions off the involved ion exchange medium so as to eventually cause displaced sample ions to appear in the effluent from the ion exchange medium; there is utilized an eluent containing said monitor/displacing ions which are photometrically detectable in said effluent and which are of the same charge but not necessarily the same valence as the sample ions to be detected; said effluent is photometrically monitored and the concentration of transparent sample ions of interest eluting from the ion exchange medium is indirectly quantitated based on the fluctuation(s) caused by same in the effluent response as produced by the appearance of the transparent sample ions in the effluent causing a concurrent and proportional dip in the concentration of the observed monitor/displacing ions in the effluent; wherein the improvement comprises: using a pH dependent variable capacity ion exchange medium that is weakly basic in nature when anions are to be detected and determined from said sample making contact with said medium and using a weakly acidic ion exchange medium when cations are so involved; and using an eluent, the pH of which controls the capacity of the ion exchange medium to a capacity within the range encompassed on the lower end, by the condition which produces overloading and on the upper end, by a capacity which is not greater than about 50 percent of the maximum potential ion exchange capacity of the ion exchange medium whereby the pH of the eluent controls the capacity of the ion exchange medium without detrimentally affecting efficiency of separation.

Claim 8

The method as set forth in any of claims 1, 2, 4 or 6 wherein the improvement further comprises utilizing a balance factor, as defined by the equation: ##EQU5## wherein: A=the pH of the eluent; B=the inflection point, in terms of A, of the curve of K' of an ion of interest in an analysis verses A for a flixed eluent displacing ion concentration used for the analysis and; C=the molar concentration of the eluent displacing ion used for the analysis, which is maintained between about 1 to about -3.

Claim 10

The method as set forth in any of claims 1, 2, 4 or 6 wherein the improvement further comprises utilizing a balance factor, as defined by the equation: ##EQU6## wherein: A=the pH of the eluent; B=the inflection point, in terms of A, of the curve of K' of an ion of interest in an analysis verses A for a fixed eluent displacing ion concentration used for the analysis and; C=the molar concentration of the eluent displacing ion used for the analysis, which is maintained between about 0 to about -2.

Claim 12

The method as set forth in any of claims 1, 2, 4 or 6 wherein the improvement further comprises adjusting the capacity of the ion exchange medium between about 0.1 to about 30 percent of the maximum potential ion exchange capacity of the medium.