US4605630A — Large-liposome agglutination reagent and method

Claim 1

Independent Claim 102 words

An agglutination assay reagent comprising liposomes prepared by forming a dispersion of lipid vesicles having heterogeneous sizes both less than and greater than 1 micron, and treating the dispersion to remove vesicles whose sizes are less than about 1 micron, each liposome having a surface array of ligand molecules at a surface concentration effective to produce liposome agglutination within about 5 minutes, when the reagent is incubated at room temperature with a multivalent ligand-binding analyte on a slide, and a dye trapped by the liposomes at a concentration effective to allow said agglutination to be visualized directly without magnification on a slide.

Claim 2

Dependent Claim 102 words

An agglutination assay reagent comprising liposomes prepared by forming a dispersion of lipid vesicles having heterogeneous sizes both less than and greater than 1 micron, and treating the dispersion to remove vesicles whose sizes are less than about 1 micron, each liposome having a surface array of ligand molecules at a surface concentration effective to produce liposome agglutination within about 5 minutes, when the reagent is incubated at room temperature with a multivalent ligand-binding analyte on a slide, and a dye trapped by the liposomes at a concentration effective to allow said agglutination to be visualized directly without magnification on a slide.

Claim 3

Dependent Claim 35 words

The reagent of claim 1, wherein the liposomes are predominantly in the 1 to 12 micron size range, and the percentage of liposomes having diameters less than about 1 micron is less than about 25%.

Claim 4

Dependent Claim 28 words

The reagent of claim 1, wherein the ligand is cardiolipin contained in the lipid phase of the liposomes, at a molar concentration of between about 1% and 20%.

Claim 5

Dependent Claim 22 words

The reagent of claim 1, wherein the interior spaces of liposomes are substantially filled with a semi-rigid gel or a polymerized matrix.

Claim 6

Dependent Claim 13 words

The reagent of claim 4, wherein the semi-rigid gel includes agar or agarose.

Claim 7

Dependent Claim 113 words

An agglutination assay reagent for determination of serum or plasma reagin antibody comprising liposomes containing cardiolipin at a molar concentration between about 1 and 20% and prepared by (a) forming a dispersion of lipid vesicles having heterogeneous sizes both less than and greater than 1 micron, and (b) treating the dispersion to remove vesicles whose sizes are less than about 1 micron, wherein incubation of the liposomes in the presence of reagin antibody at room temperature for 5 minutes is effective to produce liposome liposome agglutination on a slide, and a dye trapped by the liposomes at a concentration effective to allow said liposomes to be visualized directly without magnification on a slide.

Claim 8

Dependent Claim 113 words

An agglutination assay reagent for determination of serum or plasma reagin antibody comprising liposomes containing cardiolipin at a molar concentration between about 1 and 20% and prepared by (a) forming a dispersion of lipid vesicles having heterogeneous sizes both less than and greater than 1 micron, and (b) treating the dispersion to remove vesicles whose sizes are less than about 1 micron, wherein incubation of the liposomes in the presence of reagin antibody at room temperature for 5 minutes is effective to produce liposome liposome agglutination on a slide, and a dye trapped by the liposomes at a concentration effective to allow said liposomes to be visualized directly without magnification on a slide.

Claim 9

Dependent Claim 17 words

The reagent of claim 6, wherein the dye is encapsulated with the interior spaces of the liposomes.

Claim 10

Dependent Claim 14 words

The reagent of claim 7, wherein dye is carried on the liposome outer surfaces.

Claim 11

Dependent Claim 95 words

A rapid agglutination assay method for determination of a multivalent ligand-binding analyte, said method comprising providing a suspension of liposomes (a) prepared by forming a dispersion of lipid vesicles having heterogeneous sizes both less than and greater than 1 micron, and treating the dispersion to remove vesicles whose sizes are less than about 1 micron, and (b) having a surface array of ligand molecules and a trapped dye, and mixing the suspension with the analyte on a slide to produce liposome agglutination which is readily detectable, without magnification, within about five minutes at room temperature.

Claim 12

Dependent Claim 95 words

A rapid agglutination assay method for determination of a multivalent ligand-binding analyte, said method comprising providing a suspension of liposomes (a) prepared by forming a dispersion of lipid vesicles having heterogeneous sizes both less than and greater than 1 micron, and treating the dispersion to remove vesicles whose sizes are less than about 1 micron, and (b) having a surface array of ligand molecules and a trapped dye, and mixing the suspension with the analyte on a slide to produce liposome agglutination which is readily detectable, without magnification, within about five minutes at room temperature.

Claim 13

Dependent Claim 17 words

The method of claim 9, wherein liposomes having diameters below about 1 micron are removed by centrifugation.

Claim 14

Dependent Claim 26 words

The method of claim 9, for use in determining reagin antibody, wherein the liposomes are prepared to include between about 1 and 20 mole percent cardiolipin.

Claim 15

Dependent Claim 27 words

The method of claim 9 wherein said preparing includes forming said liposomes to encapsulate as aqueous fluid material which can be gelled or polymerized after liposome formation.

Claim 16

Dependent Claim 14 words

The method of claim 12, wherein the aqueous fluid material includes agar or agarose.

Claim 17

Dependent Claim 27 words

The method of claim 9, for use in determining a multivalent antigen, wherein the ligand includes anti-ligand antibodies covalently attached to surface lipid components in the vesicles.

Claim 18

Dependent Claim 21 words

The method of claim 14, for determination of hepatitis B surface antigen, wherein the antibodies include anti-hepatitis B surface antigen antibodies.

Claim 19

Dependent Claim 96 words

A rapid-agglutination assay method for determination of reagin antibody comprising providing a suspension of reagent particles composed of liposomes containing cardiolipin at a molar concentration between about 1%-20% and a trapped dye and prepared by (a) forming a dispersion of lipid vesicles both having heterogeneous sizes less than and greater than 1 micron, and (b) treating the dispersion to remove vesicles whose size is less than about 1 micron, and mixing the reagent with a sample of the antibody to produce agglutination which is readily detectable, without magnification, after 2 to 5 minutes at room temperature.

Claim 20

Dependent Claim 96 words

A rapid-agglutination assay method for determination of reagin antibody comprising providing a suspension of reagent particles composed of liposomes containing cardiolipin at a molar concentration between about 1%-20% and a trapped dye and prepared by (a) forming a dispersion of lipid vesicles both having heterogeneous sizes less than and greater than 1 micron, and (b) treating the dispersion to remove vesicles whose size is less than about 1 micron, and mixing the reagent with a sample of the antibody to produce agglutination which is readily detectable, without magnification, after 2 to 5 minutes at room temperature.

Claim 21

Dependent Claim 21 words

The method of claim 16, wherein the liposomes contain phosphatidyl choline, cardiolipin and cholesterol at a molar ratio of about 10:1:4.

Claim 22

Dependent Claim 14 words

The method of claim 16, which further includes adding heparin to the reagent/sample mixture.

Claim 23

Dependent Claim 66 words

In an assay procedure which includes reacting multivalent ligand-binding molecules with a suspension of ligand-coated liposomes having heterogeneous sizes both greater and less than about 1 micron, to produce liposome agglutination in proportion to the concentration of ligand-binding molecules, the improvement comprising: enhancing the rate and extent of liposome agglutination by treating the liposome suspension to remove liposomes whose sizes are less than about 1 micron.

Claim 24

Dependent Claim 66 words

In an assay procedure which includes reacting multivalent ligand-binding molecules with a suspension of ligand-coated liposomes having heterogeneous sizes both greater and less than about 1 micron, to produce liposome agglutination in proportion to the concentration of ligand-binding molecules, the improvement comprising: enhancing the rate and extent of liposome agglutination by treating the liposome suspension to remove liposomes whose sizes are less than about 1 micron.

Claim 25

Dependent Claim 25 words

The method of claim 19, wherein the treated liposome suspension contains less than about 25% of liposomes having diameters of less than about 1 micron.

Claim 26

Dependent Claim 32 words

The method of claim 19, wherein the liposome population is produced by preparing a suspension of heterogeneous-sized liposomes, and removing liposomes whose sizes are less than about 1.0 microns preferentially by centrifugation.

Claim 27

Dependent Claim 32 words

The method of claim 19, wherein the lipids are prepared to include a trapped dye, at a concentration thereof which allos agglutinated liposomes in such population to be visualized readily without magnification.

Claim 28

Dependent Claim 91 words

An agglutination assay reagent comprising liposomes predominantly in the 1-20 micron size range, wherein the interior liposome spaces are substantially filled with a semi-rigid gel or a polymerized matrix, each liposome having a surface array of ligand molecules at a surface concentration effective to produce liposome agglutination within about 5 minutes, when the reagent is incubated at room temperature with a multivalent ligand-binding analyte on a slide, and a dye trapped by the liposomes at a concentration adapted to allow such agglutination to be visualized readily without magnification on a slide.

Claim 29

Dependent Claim 91 words

An agglutination assay reagent comprising liposomes predominantly in the 1-20 micron size range, wherein the interior liposome spaces are substantially filled with a semi-rigid gel or a polymerized matrix, each liposome having a surface array of ligand molecules at a surface concentration effective to produce liposome agglutination within about 5 minutes, when the reagent is incubated at room temperature with a multivalent ligand-binding analyte on a slide, and a dye trapped by the liposomes at a concentration adapted to allow such agglutination to be visualized readily without magnification on a slide.

Claim 30

Dependent Claim 13 words

The reagent of claim 23, wherein the semi-rigid gel includes agar or agarose.

Claim 31

Dependent Claim 86 words

A rapid agglutination assay method for determination of a multivalent ligand-binding analyte, said method comprising providing a suspension of reagent particles composed of liposomes primarily in the 1-20 micron size range, each liposome having a surface array of ligand molecules and a trapped dye, wherein the interior liposome spaces are substantially filled with a semi-rigid gel or polymerized matrix, and mixing the suspension with the analyte on a slide to produce particle agglutination which is readily detectable, without magnification, within about 5 minutes at room temperature.

Claim 32

Dependent Claim 86 words

A rapid agglutination assay method for determination of a multivalent ligand-binding analyte, said method comprising providing a suspension of reagent particles composed of liposomes primarily in the 1-20 micron size range, each liposome having a surface array of ligand molecules and a trapped dye, wherein the interior liposome spaces are substantially filled with a semi-rigid gel or polymerized matrix, and mixing the suspension with the analyte on a slide to produce particle agglutination which is readily detectable, without magnification, within about 5 minutes at room temperature.

Claim 33

Dependent Claim 13 words

The method of claim 25, wherein the semi-rigid gel includes agar or agarose.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1989-08-11	1990-02-10	1990-08-12	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1993-08-11	1994-02-10	1994-08-12	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1997-08-11	1998-02-10	1998-08-12	$7700.00	Window Closed / Lapsed

Large-liposome agglutination reagent and method

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