US3949065A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Composition and method for the detection of syphilis
Assignee: IL Holding Spa
•
Filing: 1973-09-11
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Granted: 1976-04-06
•
Expires: 1993-09-11
Abstract
Abstract An antigen preparation for use in the detection of syphilis and other treponemal diseases comprising (1) V.D.R.L. or U.S.R., antigen reagent and (2) A. A COMPOUND HAVING THE FORMULA B. A COMPOUND HAVING THE FORMULA C. A COMPOUND HAVING THE FORMULA OR (D) MIXTURES OF (A) + (B) OR (A) + (C), AND (3) WHICH MAY ADDITIONALLY INCLUDE A POLYMERIC FLOCCULATING AGENT. A method of using said antigen preparation and a test card having a flocculating agent deposited thereon, are also disclosed.
Inventors
- 👤 Peter Salvatore Forgione
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 15:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/571; C; C09; C09B; C09B31/00; C09B31/02; C09B31/12; C09B31/14; C09B35/00; C09B35/02; C09B35/039; C09B35/04; Y; Y10; Y10S; Y10S436/00; Y10S436/805
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
111 words
An antigen preparation for use in carrying out an agglutination test for syphilis which consists essentially of I. from about 0.5 to about 25.0%, by weight, based on the total weight of said preparation, of a syphilis antigen reagent, Ii. from about 0.0001 to about 0.2%, by weight, same basis, of a dye compound having the formula ##SPC5## d. mixtures of (a) and (b) or (a) and (c) and Iii. from about 0.0001% to about 0.05%, by weight, same basis, of a polymeric flocculating agent selected from the group consisting of polyvinylimidazoline, a polyacrylate, an acrylamide polymer and a styrene-maleic anhydride copolymer, the remainder of said preparation being solvent and buffer.
Claim 2
Dependent Claim
111 words
An antigen preparation for use in carrying out an agglutination test for syphilis which consists essentially of I. from about 0.5 to about 25.0%, by weight, based on the total weight of said preparation, of a syphilis antigen reagent, Ii. from about 0.0001 to about 0.2%, by weight, same basis, of a dye compound having the formula ##SPC5## d. mixtures of (a) and (b) or (a) and (c) and Iii. from about 0.0001% to about 0.05%, by weight, same basis, of a polymeric flocculating agent selected from the group consisting of polyvinylimidazoline, a polyacrylate, an acrylamide polymer and a styrene-maleic anhydride copolymer, the remainder of said preparation being solvent and buffer.
Claim 3
Dependent Claim
14 words
The antigen preparation of claim 1 wherein said dye compound has the formula ##SPC6##
Claim 4
Dependent Claim
14 words
The antigen preparation of claim 1 wherein said dye compound has the formula ##SPC7##.
Claim 5
Dependent Claim
16 words
The antigen preparation of claim 1 wherein said dye is a mixture of (a) and (b).
Claim 6
Dependent Claim
14 words
The antigen preparation of claim 1 wherein said dye compound has the formula ##SPC8##.
Claim 7
Dependent Claim
16 words
The antigen preparation of claim 1 wherein said dye is a mixture of (a) and (c).
Claim 8
Dependent Claim
27 words
A method for carrying out an agglutination test for syphilis which comprises contacting (1) a test serum or plasma with (2) the antigen preparation of claim 1.
Claim 9
Dependent Claim
13 words
The method of claim 7 wherein said dye compound has the formula ##SPC9##.
Claim 10
Dependent Claim
12 words
The method of claim 7 wherein said dye has the formula ##SPC10##.
Claim 11
Dependent Claim
15 words
The method of claim 7 wherein said dye is a mixture of (a) and (b).
Claim 12
Dependent Claim
13 words
The method of claim 7 wherein said dye compound has the formula ##SPC11##.
Claim 13
Dependent Claim
15 words
The method of claim 7 wherein said dye is a mixture of (a) and (c).
Claim 14
Dependent Claim
89 words
An antigen preparation for use in carrying out an agglutination test for syphilis which consists essentially of, I. from about 0.5 to about 25.0%, by weight, based on the total weight of the preparation, of a syphilis antigen reagent, and Ii. from about 0.0001 to about 0.2%, by weight, same basis, of a dye comprising: a. a mixture of ##SPC12## the ratio of the components of said mixtures ranging from about 9 : 1 to about 1 : 9, respectively, the remainder of said preparation being solvent and buffer.
Claim 15
Dependent Claim
89 words
An antigen preparation for use in carrying out an agglutination test for syphilis which consists essentially of, I. from about 0.5 to about 25.0%, by weight, based on the total weight of the preparation, of a syphilis antigen reagent, and Ii. from about 0.0001 to about 0.2%, by weight, same basis, of a dye comprising: a. a mixture of ##SPC12## the ratio of the components of said mixtures ranging from about 9 : 1 to about 1 : 9, respectively, the remainder of said preparation being solvent and buffer.
Claim 16
Dependent Claim
12 words
The antigen preparation of claim 13 wherein said dye comprises mixture (a).
Claim 17
Dependent Claim
12 words
The antigen preparation of claim 13 wherein said dye comprises mixture (b).
Claim 18
Dependent Claim
46 words
A method for carrying out an agglutination test for syphilis which comprises mixing (1) a test serum or plasma, and (2) the antigen preparation of claim 13 with (3) a dried test spot comprising a polymeric flocculating agent on a solid sheet having a smooth surface.
Claim 19
Dependent Claim
27 words
A method for carrying out an agglutination test for syphilis which comprises contacting (1) a test serum or plasma with (2) the antigen preparation of claim 13.
Claim 20
Dependent Claim
11 words
The method of claim 17 wherein said dye comprises mixture (a).
Claim 21
Dependent Claim
11 words
The method of claim 17 wherein said dye comprises mixture (b).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-06 | 1979-10-06 | 1980-04-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-06 | 1983-10-06 | 1984-04-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-06 | 1987-10-06 | 1988-04-06 | $7700.00 | Window Closed / Lapsed |