US4483929A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Liposomes with glycolipid-linked antibodies
Assignee: Liposome Technology Inc
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Filing: 1982-05-03
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Granted: 1984-11-20
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Expires: 2002-05-03
Abstract
Abstract Lipid vesicles, labelled with encapsulated reporter compositions and bound to antibodies comprise a new class of immunoreagent, useful in immunoassays for ligands.
Inventors
- 👤 Frank C. Szoka
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 18:22
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/58; G01N33/585; G01N33/586; Y; Y10; Y10S; Y10S435/00; Y10S435/966; Y10S436/00; Y10S436/80; Y10S436/803; Y10S436/819; Y10S436/828; Y10S436/829
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
65 words
An immunoreactive liposome reagent for use in the determination of a chemical compound capable of entering into an immunospecific reaction with a known antibody, said reagent comprising a suspension of liposomes containing reporter molecules, and a surface array of molecules of such antibody attached to the liposomes through glycolipid molecules anchored in the liposomes and linked to the antibody molecules by a diimidazole coupling agent.
Claim 2
Dependent Claim
65 words
An immunoreactive liposome reagent for use in the determination of a chemical compound capable of entering into an immunospecific reaction with a known antibody, said reagent comprising a suspension of liposomes containing reporter molecules, and a surface array of molecules of such antibody attached to the liposomes through glycolipid molecules anchored in the liposomes and linked to the antibody molecules by a diimidazole coupling agent.
Claim 3
Dependent Claim
18 words
The reagent of claim 1, wherein the glycolipid is a melibionamide and the diimidazole coupling agent is 1,1-carbonyldiimidazole.
Claim 4
Dependent Claim
10 words
The reagent of claim 2, wherein the melibionamide is tetradecylmelibionamide.
Claim 5
Independent Claim
66 words
A method of producing an immunoreactive liposome reagent having an array of surface-bound antibody molecules, said method comprising providing a glycolipid, linking such antibody molecules covalently to molecules of the glycolipid, in an aqueous medium, with a diimidazole coupling agent, and mixing the resultant antibody-glycolipid couple with liposomes in an aqueous medium to produce binding of the antibody to the liposome surface through the linked glycolipid.
Claim 6
Independent Claim
66 words
A method of producing an immunoreactive liposome reagent having an array of surface-bound antibody molecules, said method comprising providing a glycolipid, linking such antibody molecules covalently to molecules of the glycolipid, in an aqueous medium, with a diimidazole coupling agent, and mixing the resultant antibody-glycolipid couple with liposomes in an aqueous medium to produce binding of the antibody to the liposome surface through the linked glycolipid.
Claim 7
Dependent Claim
12 words
The method of claim 4, wherein the glycolipid provided is a melibionamide.
Claim 8
Dependent Claim
11 words
The method of claim 5, wherein the melibionamide provided is tetradecylmelibionamide.
Claim 9
Dependent Claim
10 words
The method of claim 5, wherein the diimidazole is 1,1-carbonyldiimidazole.
Claim 10
Dependent Claim
16 words
The method of claim 5, wherein said linking is performed in the absence of a detergent.
Claim 11
Dependent Claim
26 words
The method of claim 8, wherein said mixing is also performed in the absence of a detergent to produce a liposome reagent free of contaminating detergent.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-11-20 | 1988-05-21 | 1988-11-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-11-20 | 1992-05-21 | 1992-11-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-11-20 | 1996-05-21 | 1996-11-20 | $7700.00 | Window Closed / Lapsed |