US4235792A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Immunological materials
Assignee: University of Toronto
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Filing: 1977-04-14
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Granted: 1980-11-25
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Expires: 1997-04-14
Abstract
Abstract Immune lysis of sensitized liposomes containing a marker is employed in an assay method wherein an antigenic material to be determined is reacted with a specific antiserum and the degree of inhibition of the antiserum is detected by the extent of release of a marker when the antiserum reacts with liposomes containing a sensitizer specific to the antiserum.
Inventors
- 👤 Jen C. Hsia
- 👤 Chou-Tok Tan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/74; G01N33/78; C; C07; C07F; C07F9/00; C07F9/02; C07F9/06; C07F9/08; C07F9/09; C07F9/091; C07J; C07J41/00; C07J41/0033; C07J41/0055; C07J51/00; G01N33/58; G01N33/585; G01N33/586; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/816; Y10S436/817; Y10S436/829
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
69 words
A synthetic sensitizer for use in forming sensitized liposomes and characterized by a molecular structure comprising an antigenic radical selected from the group consisting of thyroxine-N-group, and 3,5,3'-triiodo-thyronine-N-group linking to the 5-position of a 2,4-dinitro-phenylene group and said phenylene group being linked at the 1-position to an amphiphilic radical selected from the group consisting of phosphatidyl esters of hydroxyamines, amino-acylated phosphatidyl esters of hydroxy amines, cholesterols, sphingosins and dihydrosphingosins.
Claim 2
Dependent Claim
69 words
A synthetic sensitizer for use in forming sensitized liposomes and characterized by a molecular structure comprising an antigenic radical selected from the group consisting of thyroxine-N-group, and 3,5,3'-triiodo-thyronine-N-group linking to the 5-position of a 2,4-dinitro-phenylene group and said phenylene group being linked at the 1-position to an amphiphilic radical selected from the group consisting of phosphatidyl esters of hydroxyamines, amino-acylated phosphatidyl esters of hydroxy amines, cholesterols, sphingosins and dihydrosphingosins.
Claim 3
Dependent Claim
25 words
A sensitizer as claimed in claim 1 wherein said amphiphilic radical is selected from the group consisting of phosphatidylethanolamines, aminocarboxyl phosphatidylethanolamines, and N,N'-bis (dinitro-halo-phenyl)-diaminocarboxyl phosphatidylethanolamine.
Claim 4
Dependent Claim
113 words
A sensitizer as claimed in claim 1 wherein said amphiphilic radical is selected from the group consisting of (a) phosphatidylethanolamines of the formula ##STR14## wherein R and R' are independently H, OH, R" or ##STR15## wherein R" is a saturated or unsaturated branched or straight chain alkyl or alkylene group of 1 to 24 carbons, and wherein at least one of R and R' is ##STR16## or OR"; (b) aminocapropyl phosphatidylethanolamines of the formula ##STR17## wherein R and R' are as defined above; and (c) divalent lysyl phosphatidylethanolamine of the formula ##STR18## wherein R and R' are as defined above and each NH-- group is linked through a 2,4-dinitrophenylene group to said antigen.
Claim 5
Dependent Claim
9 words
A sensitizer comprising the compound 2,4-dinitro-5-(thyroxine-N-) phenylated dipalmitoyl phosphatidylethanolamine.
Claim 6
Dependent Claim
9 words
A sensitizer comprising the compound 2,4-dinitro-5-(thyroxine-N-) phenylated dipalmitoyl phosphatidylethanolamine.
Claim 7
Dependent Claim
147 words
A sensitizer characterized by a molecular structure consisting of an antigen group selected from the group consisting of thyroxine-N-group and 3,5,3'-triiodothyronine-N-group linked to the 4-position of 3,3'-dinitrophenylene sulfone residue of which the 4' position is linked to an amphiphilic group selected from the group consisting of (a) phosphatidylethanolamines of formula ##STR19## wherein R and R' are independently H, OH, R", OR" or ##STR20## wherein R" is a saturated or unsaturated, branched or straight chain alkyl or alkylene group of 1 to 24 carbons, and wherein at least one of R and R' is ##STR21## or OR"; (b) aminocaproyl phosphatidylethanolamines of formula ##STR22## wherein R and R' are as defined above; and (c) divalent lysyl phosphatidylethanolamine of formula ##STR23## wherein R and R' are as defined above and each NH-- group is linked through the 4- and 4'- position of a 3,3'-dinitrophenylene sulfone residue to said antigen group.
Claim 8
Dependent Claim
147 words
A sensitizer characterized by a molecular structure consisting of an antigen group selected from the group consisting of thyroxine-N-group and 3,5,3'-triiodothyronine-N-group linked to the 4-position of 3,3'-dinitrophenylene sulfone residue of which the 4' position is linked to an amphiphilic group selected from the group consisting of (a) phosphatidylethanolamines of formula ##STR19## wherein R and R' are independently H, OH, R", OR" or ##STR20## wherein R" is a saturated or unsaturated, branched or straight chain alkyl or alkylene group of 1 to 24 carbons, and wherein at least one of R and R' is ##STR21## or OR"; (b) aminocaproyl phosphatidylethanolamines of formula ##STR22## wherein R and R' are as defined above; and (c) divalent lysyl phosphatidylethanolamine of formula ##STR23## wherein R and R' are as defined above and each NH-- group is linked through the 4- and 4'- position of a 3,3'-dinitrophenylene sulfone residue to said antigen group.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-25 | 1984-05-26 | 1984-11-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-25 | 1988-05-26 | 1988-11-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-25 | 1992-05-26 | 1992-11-25 | $7700.00 | Window Closed / Lapsed |