US3882225A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Direct agglutination immunological reagent
Assignee: Bayer Corp
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Filing: 1968-12-09
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Granted: 1975-05-06
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Expires: 1988-12-09
Abstract
Abstract Immunological reagents capable of direct agglutination with antigens are prepared by pre-polymerizing the antibody to form an aggregate and then coupling this aggregate to a microbial cell carrier particle with a chemical coupling agent. The prepolymerization of the antibody can be accomplished by the use of the same or another coupling agent. The use of the microbial cell carrier particle allows the reagents formed to be used as immunological indicator particles in slide tests which permit a quick read-out of test results. The reagent formed may be prepared and shipped in dry form and then reconstituted with liquid for use.
Inventors
- 👤 Virendra Patel
- 👤 Marion Cook Fetter
- 👤 Shradha Nand
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 17:37
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
99 words
AN IMMUNOLOGICAL INDICATOR REAGENT CAPABLE OF DETECTING AN ANTIGEN SELECTED FROM THE GROUP CONSISTING OF BOVINE SERUM ALBUMIN, DIPTHERIA TOXOID, TETANUS TOXOID, AND C-REACTIVE PROTEIN, COMPRISING A PRE-POLYMERIZED AGGREGATE OF AN ANTIBODY COUPLED TO A BACTERIAL CELL WITH A COUPLING AGENT SAID; BACTERIAL CELL BEING OF UNIFORM SHAPE AND SIZE SELECTED FROM THE GROUP CONSISTING OF E. COLI, B. SUBTILIS, L. LEICHMANNII, B. PUMILUS AND P. FRAGI SAID COUPLING AGENT BEING SELECTED FROM THE GROUP CONSISTING OF BIS DIAZOBENZIDINE, BIS DIAZOBENZIDINE DISULFONIC ACID, ACROLEIN, DIFLUORODINITROBENZENE, DICYCLOHEXYLCARBODIIMIDE, CYANURIC CHLORIDE, 2,7-DIAMINOFLUORENE, TOLUENE DIISOCYANATE, DICHLOROTRIAZINE, DIFLUORODINITRODIPHENYL SULFONE, TETRAZO-P-PHENYLENEDIAMINE, GLUTARALDEHYDE AND N-5BUTYL-5-METHYLISOXAZOLIUM PERCHLORATE.
Claim 2
Dependent Claim
99 words
AN IMMUNOLOGICAL INDICATOR REAGENT CAPABLE OF DETECTING AN ANTIGEN SELECTED FROM THE GROUP CONSISTING OF BOVINE SERUM ALBUMIN, DIPTHERIA TOXOID, TETANUS TOXOID, AND C-REACTIVE PROTEIN, COMPRISING A PRE-POLYMERIZED AGGREGATE OF AN ANTIBODY COUPLED TO A BACTERIAL CELL WITH A COUPLING AGENT SAID; BACTERIAL CELL BEING OF UNIFORM SHAPE AND SIZE SELECTED FROM THE GROUP CONSISTING OF E. COLI, B. SUBTILIS, L. LEICHMANNII, B. PUMILUS AND P. FRAGI SAID COUPLING AGENT BEING SELECTED FROM THE GROUP CONSISTING OF BIS DIAZOBENZIDINE, BIS DIAZOBENZIDINE DISULFONIC ACID, ACROLEIN, DIFLUORODINITROBENZENE, DICYCLOHEXYLCARBODIIMIDE, CYANURIC CHLORIDE, 2,7-DIAMINOFLUORENE, TOLUENE DIISOCYANATE, DICHLOROTRIAZINE, DIFLUORODINITRODIPHENYL SULFONE, TETRAZO-P-PHENYLENEDIAMINE, GLUTARALDEHYDE AND N-5BUTYL-5-METHYLISOXAZOLIUM PERCHLORATE.
Claim 3
Dependent Claim
26 words
A reagent as in claim 16 capable of detecting bovine serum albumin comprising the pre-polymerized aggregate of an antibody covalently bound to E. coli with acrolein
Claim 4
Dependent Claim
26 words
A reagent as in claim 1 capable of detecting diptheria toxoid comprising the pre-polymerized aggregate of an antibody covalently bound to E. coli with bis diazobenzidine.
Claim 5
Dependent Claim
26 words
A reagent as in claim 1 capable of detecting tetanus toxoid comprising the pre-polymerized aggregate of an antibody covalently bound to E. coli with bis diazobenzidine.
Claim 6
Dependent Claim
32 words
A reagent as in claim capable of detecting C-reactive protein comprising the capable of detecting C-reactive protein comprising the pre-polymerized aggregate of an antibody covalently bound to E. coli with bis diazobenzidine.
Claim 7
Dependent Claim
26 words
A reagent as in claim 1 capable of detecting bovine serum albumin comprising the pre-polymerized aggregate of an antibody covalently bound to E. coli with 2,7-diaminofluorene.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-05 | 1978-11-04 | 1979-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-05 | 1982-11-04 | 1983-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-05 | 1986-11-04 | 1987-05-06 | $7700.00 | Window Closed / Lapsed |