US4559307A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Treatment of yeast cells with proteolytic enzymes
Assignee: Burns Philp Food Inc
•
Filing: 1983-10-17
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Granted: 1985-12-17
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Expires: 2003-10-17
Abstract
Abstract Functional protein having reduced nucleic acid content is produced without initial denaturation of the protein by contacting undenatured yeast cells with an alkaline protease at a temperature of about 20° C. to 40° C. for about 2 minutes to 2 hours at a pH of about 8 to 11. The yeast cells are preferably Pichia pastoris and the alkaline protease is preferably from Bacillus lichenformis.
Inventors
- 👤 Thomas R. Hopkins
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 21:38
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/08; C12N1/005; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/938
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
53 words
A process for the recovery of functional protein from undenatured yeast cells which comprises contacting said undenatured yeast cells with an alkaline protease at a temperature of from about 20° C. to about 40° C. for about 2 minutes to 2 hours and at a pH of from about 8 to about 11.
Claim 2
Dependent Claim
53 words
A process for the recovery of functional protein from undenatured yeast cells which comprises contacting said undenatured yeast cells with an alkaline protease at a temperature of from about 20° C. to about 40° C. for about 2 minutes to 2 hours and at a pH of from about 8 to about 11.
Claim 3
Dependent Claim
15 words
A process according to claim 1 wherein said yeast cells are from the genera Pichia.
Claim 4
Dependent Claim
13 words
A process according to claim 2 wherein said yeast cells are Pichia pastoris.
Claim 5
Dependent Claim
16 words
A process according to claim 1 wherein said alkaline protease is derived from the genera Bacillus.
Claim 6
Dependent Claim
15 words
A process according to claim 4 wherein said alkaline protease is derived from Bacillus lichenformis.
Claim 7
Dependent Claim
20 words
A process according to claim 1 carried out at a temperature of from about 35° C. to about 40° C.
Claim 8
Dependent Claim
13 words
A process according to claim 1 carried out at a pH of 9.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-16 | 1989-06-17 | 1989-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-16 | 1993-06-17 | 1993-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-16 | 1997-06-17 | 1997-12-17 | $7700.00 | Window Closed / Lapsed |