US3997397A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of proteic materials from yeast cells
Assignee: Societa Italiana Resine SpA SIR
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Filing: 1975-04-09
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Granted: 1976-12-14
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Expires: 1995-04-09
Abstract
Abstract Proteic material is produced from yeast cells without prior treatment of the cells by subjecting them to lysis action by means of an enzyme complex obtained from strains of the genus Cytophaga.
Inventors
- 👤 Renato Craveri
- 👤 Cesarina Colla
- 👤 Valeria Cavazzoni
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 07:33
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N1/00; C12N1/06; C12N1/063; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/923; Y10S435/93; Y10S435/94; Y10S530/00; Y10S530/82; Y10S530/823; Y10S530/824
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
111 words
A process for producing proteic material from live yeast cells selected from the group consisting of Candida lipolytica, Candida vini, Candida utilis, Hanseniaspora valbyensis, Kloeckera apiculata, Kloeckera africana, Kloeckera sp., and Saccharomycodes ludwigii comprising (1) preparing a culture filtrate for use as a lytic factor for said yeast cells by growing a submerged culture of bacteria of the genus Cytophaga on a culture soil comprising live cells of yeast of the same species as that to be subsequently subject to lysis and (2) then utilizing directly the resulting culture filtrate as a lytic factor for the lysis of biomasses of said live yeasts, without subjecting said live yeast to any pretreatment.
Claim 2
Dependent Claim
111 words
A process for producing proteic material from live yeast cells selected from the group consisting of Candida lipolytica, Candida vini, Candida utilis, Hanseniaspora valbyensis, Kloeckera apiculata, Kloeckera africana, Kloeckera sp., and Saccharomycodes ludwigii comprising (1) preparing a culture filtrate for use as a lytic factor for said yeast cells by growing a submerged culture of bacteria of the genus Cytophaga on a culture soil comprising live cells of yeast of the same species as that to be subsequently subject to lysis and (2) then utilizing directly the resulting culture filtrate as a lytic factor for the lysis of biomasses of said live yeasts, without subjecting said live yeast to any pretreatment.
Claim 3
Dependent Claim
46 words
The process defined in claim 1, wherein the lysis process is carried out in a fermenter by adding to a lysis-producing culture filtrate a suspension of live yeast cells at temperatures from 40° to 45° C under rotary agitation for a processing time of 2-4 hours.
Claim 4
Dependent Claim
18 words
The process defined in claim 1, wherein the yeasts employed are obtained by culturing on a paraffin substrate.
Claim 5
Dependent Claim
18 words
The process defined in claim 1, wherein the yeasts employed are obtained by culturing on a molasses substrate.
Claim 6
Dependent Claim
19 words
The process defined in claim 1, wherein the yeasts employed are obtained by culturing on an aliphatic alcohol substrate.
Claim 7
Dependent Claim
49 words
The process defined in claim 1, wherein, for growing a submerged culture of the Cytophaga producing the lytic factor, of live yeast and from 0.1 to 0.5%, as dry matter, of autoclavate yeast, these yeasts being of the same species as those which will subsequently be subjected to lysis.
Claim 8
Dependent Claim
11 words
The process of claim 1 consisting essentially of said process steps.
Claim 9
Dependent Claim
30 words
The process of claim 1 wherein said bacteria of the genus Cytophaga is the strain 21B II lodged at the Institute of Industrial Micro-Biology at the Milan University of Study.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-12-14 | 1980-06-14 | 1980-12-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-12-14 | 1984-06-14 | 1984-12-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-12-14 | 1988-06-14 | 1988-12-14 | $7700.00 | Window Closed / Lapsed |