Production of protein with reduced nucleic acid

Claim 2

A process for producing nucleic acid reduced protein (NARP) from a single cell protein (SCP) mixture comprising (a) subjecting an SCP mixture containing single cells with high nucleic acid content and a liquid to a nucleic acid removal step wherein said SCP mixture is heated with a mineral acid at temperatures between about 50° C. and about 100° C. so at least a significant portion of the nucleic acid in the single cells is transferred to said liquid while the single cells remain essentially intact thus producing a mixture of cells with reduced mucleic acid content and a nucleic acid containing fluid, (b) separating said cells with reduced nucleic acid content from said nucleic acid containing fluid, (c) extracting the cells with reduced nucleic acid content with base at a pH of about 10.6 or more to obtain a NARP mixture of a liquid and a solid phase, (d) separating the liquid phase being essentially free of insoluble cellular material and the solid phase being essentially free of the liquid, (e) recovering said liquid phase as a NARP solution and the first product of the process, and (f) recovering the solid phase as a NARP cell product and the second product of the process.

Claim 3

A process in accordance with claim 1 wherein said SCP mixture is contacted with H 2 SO 4 .

Claim 4

A process in accordance with claim 1 wherein said SCP mixture is contacted with H 3 PO 4 and said first separation step (b) is carried out only after prior neutralization of said nucleic acid containing fluid.

Claim 5

A process in accordance with claim 1 wherein prior to said first separation step (b) said mixture containing nucleic acids is neutralized to a pH of about 5 and 10 and only thereafter said cells with reduced nucleic acid content are separated from said nucleic acid containing fluid.

Claim 6

A process in accordance with claim 1 wherein said SCP mixture is obtained by subjecting a mixture of a microorganism and a growth medium to fermentation conditions in a fermentation zone and said SCP mixture is at least a portion of the effluent from said fermentation zone.

Claim 7

A process in accordance with claim 5 wherein said microorganism used is a yeast.

Claim 8

A process in accordance with claim 5 wherein at least a portion of said nucleic acid containing fluid is introduced into said fermentation zone.

Claim 9

A process in accordance with claim 7 wherein said step (a) is carried out by subjecting said single cell protein mixture to a treatment with an acid and heat and wherein said acid is neutralized with an oxide, hydroxide, carbonate or bicarbonate of a metal or equivalent of metal present in said growth medium as minerals or trace minerals.

Claim 10

A process in accordance with claim 8 wherein said acid is a mineral acid, the anion of said acid being the same as the anion of said minerals or trace minerals.

Claim 11

A process in accordance with claim 8 wherein said neutralization is carried out prior to said first separation step (b).

Claim 12

A process in accordance with claim 1 wherein said extraction is carried out with a 0.5 N to 15 N base.

Claim 13

A process in accordance with claim 11 wherein said extraction is carried out with NH 4 OH.

Claim 14

A process for the production of cells of reduced nucleic acid content comprising (a) subjecting a microorganism and a minerals containing growth medium for said microorganism together to fermentation conditions in a fermentation zone, (b) withdrawing an effluent containing said microorganism and growth medium from said fermentation zone and introducing said effluent into an acidifying zone wherein said effluent is contacted with a mineral acid under suitable conditions to remove at least a portion of the nucleic acids contained in the cells of said microorganism into the surrounding fluid, (c) withdrawing from said acidifying zone an acidified mixture of a nucleic acid containing liquid phase and a solid cell phase, the cells of said solid cells phase having a reduced nucleic acid content but an essentially unchanged protein content as compared to the cells in the liquid phase. (d) separating in a separation zone the liquid phase and the solid cell phase into a cell rich phase and a liquid phase essentially free of microbial cells, (e) withdrawing said liquid phase from said separation zone, (f) neutralizing said liquid phase prior to the introduction thereof into the fermentation zone with an oxide, hydroxide, carbonate, or bicarbonate of one or more metals or equivalents of metals, the mineral acid and the metals being selected so that the mineral acid anion and the metal together will form one or more of the minerals already present or desired in said growth medium, (g) introducing at least a portion of the neutralized nucleic acid containing liquid phase into said fermentation zone, (h) subjecting the solid cell phase cells with reduced nucleic acid content to an extraction with a base at a pH of about 10.6 or higher to obtain a liquid and solid phase, (i) separating said liquid and solid phase into a liquid phase containing the extracting protein and a proteinaceous solid phase, (j) drying said liquid phase containing the extracted protein and said proteinaceous solid phase individually so as to obtain NARP products use.

Claim 15

A process for the production of cells of reduced nucleic acid content comprising (a) subjecting a microorganism and a minerals containing growth medium for said microorganism together to fermentation conditions in a fermentation zone, (b) withdrawing an effluent containing said microorganism and growth medium from said fermentation zone and introducing said effluent into an acidifying zone wherein said effluent is contacted with a mineral acid under suitable conditions to remove at least a portion of the nucleic acids contained in the cells of said microorganism into the surrounding fluid, (c) withdrawing from said acidifying zone an acidified mixture of a nucleic acid containing liquid phase and a solid cell phase, the cells of said solid cells phase having a reduced nucleic acid content but an essentially unchanged protein content as compared to the cells in the liquid phase. (d) separating in a separation zone the liquid phase and the solid cell phase into a cell rich phase and a liquid phase essentially free of microbial cells, (e) withdrawing said liquid phase from said separation zone, (f) neutralizing said liquid phase prior to the introduction thereof into the fermentation zone with an oxide, hydroxide, carbonate, or bicarbonate of one or more metals or equivalents of metals, the mineral acid and the metals being selected so that the mineral acid anion and the metal together will form one or more of the minerals already present or desired in said growth medium, (g) introducing at least a portion of the neutralized nucleic acid containing liquid phase into said fermentation zone, (h) subjecting the solid cell phase cells with reduced nucleic acid content to an extraction with a base at a pH of about 10.6 or higher to obtain a liquid and solid phase, (i) separating said liquid and solid phase into a liquid phase containing the extracting protein and a proteinaceous solid phase, (j) drying said liquid phase containing the extracted protein and said proteinaceous solid phase individually so as to obtain NARP products use.

Claim 16

A process in accordance with claim 13 wherein said mineral acid is selected from the group consisting of sulfuric acid, hydrochloric acid, phosphoric acid and said neutralization step is carried out with a base selected from the group consisting from the hydroxides or carbonates of sodium, potassium, calcium, magnesium and ammonium and mixtures thereof.

Claim 17

A process in accordance with claim 13 wherein the base in extraction step (h) is ammonium hydroxide.

Claim 18

A process in accordance with claim 15 wherein, the ammonia values are removed during product drying steps (j) are recycled back to the fermenter.