US4007088A
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Process of manufacturing native microbial protein with a low content of nucleic acids
Assignee: Czech Academy of Sciences
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Filing: 1975-08-08
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Granted: 1977-02-08
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Expires: 1995-08-08
Abstract
Abstract The invention relates to a process of manufacturing native microbial protein with a low content of nucleic acids, the product being useful as food or feed. The products obtained by the process of the invention may be widely used in the food industry, especially in the meat, milk, and bread industries. These products may replace meat or milk and can also be used in the nutrition of animals. The products may also be used as substitutes for casein, in the fortification of flour, or to improve the quality and value of bread and the like.
Inventors
- 👤 Zdenek Fencl
- 👤 Frantisek Machek
- 👤 Vladimir Sillinger
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 01:17
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/08; A; A23; A23J; A23J1/00; A23J1/008; A23J1/18; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/837; Y10S435/848; Y10S435/849; Y10S435/858; Y10S435/911; Y10S435/921; Y10S435/93; Y10S435/938; Y10S435/944; Y10S530/00; Y10S530/82; Y10S530/821; Y10S530/825
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
145 words
A process for producing native microbial protein from single-cell microbial cells having a content of nucleic acids lower than 1% and useful as food or feed, which comprises the steps of: a. disrupting microbial cells to release the nuclease contained therein and forming thereafter a homogenate having disrupted cells and nuclease; b. adjusting the pH value of the said homogenate to 5.9-8.0 and maintaining the temperature at 50°-60°C for 20 minutes-2.5 hours in order to permit the degradation of nucleic acids by means of the nucleases contained in the homogenate; c. precipitating protein material from the microbial homogenate by adjusting the pH value to the isoelectric point of the protein material; d. separating the precipitated protein material; e. drying the precipitated protein material; f. extracting the precipitated and dried protein material with alcohol to remove lipoid substances; and g. drying again said extracted protein material.
Claim 2
Dependent Claim
145 words
A process for producing native microbial protein from single-cell microbial cells having a content of nucleic acids lower than 1% and useful as food or feed, which comprises the steps of: a. disrupting microbial cells to release the nuclease contained therein and forming thereafter a homogenate having disrupted cells and nuclease; b. adjusting the pH value of the said homogenate to 5.9-8.0 and maintaining the temperature at 50°-60°C for 20 minutes-2.5 hours in order to permit the degradation of nucleic acids by means of the nucleases contained in the homogenate; c. precipitating protein material from the microbial homogenate by adjusting the pH value to the isoelectric point of the protein material; d. separating the precipitated protein material; e. drying the precipitated protein material; f. extracting the precipitated and dried protein material with alcohol to remove lipoid substances; and g. drying again said extracted protein material.
Claim 3
Dependent Claim
32 words
Process in accordance with claim 1, wherein the subject single-cell microbial cells are chosen from the group of yeasts consisting of the genus Saccharomyces cerevisiae, Candida, Torulopsis, Rhodotorula, Oiidium, Pichia, and Hansenula.
Claim 4
Dependent Claim
27 words
Process in accordance with claim 1, wherein the single-cell microbial cells are chosen from the group consisting of bacteria of the genus Megaterium, Methanomonas, and Escherichia coli.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-02-08 | 1980-08-09 | 1981-02-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-02-08 | 1984-08-09 | 1985-02-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-02-08 | 1988-08-09 | 1989-02-08 | $7700.00 | Window Closed / Lapsed |