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US4536478A
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Method for reducing non-specific interferences in agglutination immunoassays
Assignee: Seragen Diagnostics Inc
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Filing: 1984-04-05
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Granted: 1985-08-20
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Expires: 2004-04-05
Abstract
Abstract An agglutination immunoassay in which non-specific interferences are reduced by adding to the reaction mixture a halogen substituted carboxylic acid such as chloroacetic acid, dichloroacetic acid, trichloroacetic acid, bromoacetic acid, dibromoacetic acid, tribromoacetic acid, iodoacetic acid and mixtures thereof.
Inventors
- 👤 Roger L. Sokoloff
- 👤 John M. Reno
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 16:53
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S436/00; Y10S436/815; Y10S436/817; Y10S436/818; Y10S436/825
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
125 words
In an agglutination immunoassay for an analyte in a fluid sample which is combined with a reagent which includes sensitized latex particles and binding partner as required, whereby the degree of agglutination of the particles is a measure of the analyte in the sample, the improvement comprising the step of adding to the reaction mixture an additive to decrease non-specific interferences of the agglutination of the particles, where such additives comprise at least one halogen substituted carboxylic acid or water soluble salt thereof wherein the acid has the formula: ##STR2## and wherein R 1 is Cl, Br or I and R 2 is H, Cl, Br or I and R 3 is H, CH-- 3 --, CH 3 CH 2 --, Cl, Br or I.
Claim 2
Dependent Claim
125 words
In an agglutination immunoassay for an analyte in a fluid sample which is combined with a reagent which includes sensitized latex particles and binding partner as required, whereby the degree of agglutination of the particles is a measure of the analyte in the sample, the improvement comprising the step of adding to the reaction mixture an additive to decrease non-specific interferences of the agglutination of the particles, where such additives comprise at least one halogen substituted carboxylic acid or water soluble salt thereof wherein the acid has the formula: ##STR2## and wherein R 1 is Cl, Br or I and R 2 is H, Cl, Br or I and R 3 is H, CH-- 3 --, CH 3 CH 2 --, Cl, Br or I.
Claim 3
Dependent Claim
37 words
The method of claim 1 wherein the additive is selected from the group consisting of chloroacetic acid, dichloroacetic acid, trichloroacetic acid, bromoacetic acid, dibromoacetic acid, tribromoacetic acid, iodoacetic acid, a water soluble salt thereof and mixtures thereof.
Claim 4
Independent Claim
24 words
The method of any one of claims 1 or 2 wherein the additive is present in a concentration ranging from 0.01 to 2.0 molar.
Claim 5
Independent Claim
20 words
The method of any one of claims 1 or 2 wherein the additive is present as its water soluble salt.
Claim 6
Independent Claim
24 words
The method of any one of claims 1 or 2 wherein the additive composition is a mixture of at least two of said additives.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-19 | 1989-02-18 | 1989-08-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-19 | 1993-02-18 | 1993-08-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-19 | 1997-02-18 | 1997-08-20 | $7700.00 | Window Closed / Lapsed |