Patent US4534967A successfully refreshed from Google Patents.
US4534967A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell proliferation inhibitor and method of preparation
Assignee: Boston Biomedical Research Institute
•
Filing: 1982-08-03
•
Granted: 1985-08-13
•
Expires: 2002-08-03
Abstract
Abstract An inhibitor of endothelial cell growth is prepared by directly liquifying vitreous gel, as for example, forcing vitreous gel through a small orifice so as to directly convert the gel into a liquid, removing insoluble and suspended material and chromatographically fractionating the liquid and isolating the fractions. An inhibitor is also prepared by culturing hyalocyte cells in a medium and chromatographically fractionating the medium.
Inventors
- 👤 Bernard Jacobson
- 👤 Laurie Raymond
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 14:14
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/44
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
65 words
A non-extractive method for preparing an inhibitor of cell growth, comprising directly liquefying vitreous gel in the absence of extraction solvent by application of shear forces to the gel so as to directly convert the gel into a liquid, removing any insoluble and suspended material from the liquid, chromatographically separating the liquid into fractions of varying molecular weight and isolating a product having inhibitory activity.
Claim 2
Dependent Claim
65 words
A non-extractive method for preparing an inhibitor of cell growth, comprising directly liquefying vitreous gel in the absence of extraction solvent by application of shear forces to the gel so as to directly convert the gel into a liquid, removing any insoluble and suspended material from the liquid, chromatographically separating the liquid into fractions of varying molecular weight and isolating a product having inhibitory activity.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein the direct liquefaction is accomplished by forcing the gel through a small orifice.
Claim 4
Dependent Claim
16 words
The method of claim 1 wherein the direct liquefaction is accomplished by application of ultrasonic vibration.
Claim 5
Dependent Claim
13 words
The method of claim 1 wherein the product inhibits growth of endothelial cells.
Claim 6
Dependent Claim
14 words
The method of claim 1 wherein the product inhibits growth of smooth muscle cells.
Claim 7
Dependent Claim
18 words
The method of claim 1 wherein the product has a molecular weight of less than approximately 10,000 daltons.
Claim 8
Dependent Claim
16 words
The method of claim 1 wherein the product has a molecular weight of approximately 6000 daltons.
Claim 9
Dependent Claim
13 words
The method of claim 1 wherein the vitreous gel is of animal origin.
Claim 10
Dependent Claim
13 words
The method of claim 1 wherein the vitreous gel is of human origin.
Claim 11
Dependent Claim
18 words
The method of claim 1 wherein the liquid is separated into fractions by liquid chromatography utilizing an eluant.
Claim 12
Dependent Claim
11 words
The method of claim 10 wherein the eluant is physiological saline.
Claim 13
Dependent Claim
12 words
The inhibitor of cell growth made by the method of claim 1.
Claim 14
Dependent Claim
20 words
A method of treating or preventing ocular neovascularization comprising the administration to ocular tissue of the inhibitor of claim 12.
Claim 15
Dependent Claim
20 words
A method of reducing neovascularization in a tumor comprising the administration to the tumor of the inhibitor of claim 12.
References Cited (Backward Citations)
US30239A
Cited
US3864084A
Cited
US4042457A
Cited
USRE30239E
Cited
US4164560A
Cited
US4176177A
Cited
US4217412A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-08-12 | 1989-02-11 | 1989-08-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-08-12 | 1993-02-11 | 1993-08-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-08-12 | 1997-02-11 | 1997-08-13 | $7700.00 | Window Closed / Lapsed |