US4042457A
Expired / Lapsed
US
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Preparation of tissue invasion inhibitor and method of treatment utilizing the inhibitor
Assignee: Rush Presbyterian St Lukes Medical Center
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Filing: 1975-11-10
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Granted: 1977-08-16
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Expires: 1995-11-10
Abstract
Abstract A composition of matter having activity as an inhibitor of cell proliferation is obtained by aqueous extraction of tissue having a high content of collagen and/or proteoglycans.
Inventors
- 👤 Klaus E. Kuettner
- 👤 Reuben Eisenstein
- 👤 Nino Sorgente
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 07:38
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/44; A61K35/32
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
104 words
The method of preparing a composition of matter having activity as an inhibitor of cell proliferation comprising the steps of providing connective tissue having a high content of collagen or proteoglycans in condition for extraction, extracting said inhibitor from said tissue with an aqueous extraction medium which includes a solute which does not irreversibly denature the proteinaceous matter to be extracted, separating the resultant aqueous extract from the tissue, recovering from the aqueous extract substances having a molecular weight below about 50,000, treating the fraction of aqueous extract having a molecular weight below about 50,000 to remove salts therefrom, and dehydrating the resultant material.
Claim 2
Independent Claim
104 words
The method of preparing a composition of matter having activity as an inhibitor of cell proliferation comprising the steps of providing connective tissue having a high content of collagen or proteoglycans in condition for extraction, extracting said inhibitor from said tissue with an aqueous extraction medium which includes a solute which does not irreversibly denature the proteinaceous matter to be extracted, separating the resultant aqueous extract from the tissue, recovering from the aqueous extract substances having a molecular weight below about 50,000, treating the fraction of aqueous extract having a molecular weight below about 50,000 to remove salts therefrom, and dehydrating the resultant material.
Claim 3
Dependent Claim
19 words
The method of claim 1 wherein the connective tissue comprises tissue normally devoid of an intimate capillary blood supply.
Claim 4
Dependent Claim
10 words
The method of claim 2 wherein the tissue comprises cartilage.
Claim 5
Dependent Claim
11 words
The method of claim 2 wherein the tissue comprises blood vessels.
Claim 6
Dependent Claim
21 words
A composition of matter having activity as an inhibitor of cell proliferation comprising the product of the method of claim 1.
Claim 7
Dependent Claim
21 words
A composition of matter having activity as an inhibitor of cell proliferation comprising the product of the method of claim 2.
Claim 8
Dependent Claim
17 words
A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 1.
Claim 9
Dependent Claim
17 words
A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 2.
Claim 10
Dependent Claim
17 words
A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 3.
Claim 11
Dependent Claim
17 words
A method of inhibiting proliferation of cells comprising treating the cells with the product of claim 4.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-08-15 | 1981-02-14 | 1981-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-08-15 | 1985-02-14 | 1985-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-08-15 | 1989-02-14 | 1989-08-16 | $7700.00 | Window Closed / Lapsed |