US4532204A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Mutation assays involving blood cells that metabolize toxic substances
Assignee: Massachusetts Institute of Technology
•
Filing: 1982-07-19
•
Granted: 1985-07-30
•
Expires: 2002-07-19
Abstract
Abstract A line of human blood cells which have high levels of oxidative activity (such as oxygenase, oxidase, peroxidase, and hydroxylase activity) is disclosed. Such cells grow in suspension culture, and are useful to determine the mutagenicity of xenobiotic substances that are metabolized into toxic or mutagenic substances. Mutation assays using these cells, and other cells with similar characteristics, are also disclosed.
Inventors
- 👤 Charles L. Crespi
- 👤 William G. Thilly
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 10:05
CPC Classifications
C; C02; C02F; C02F1/00; C02F1/02; F; F24; F24D; F24D11/00; F24D11/02; F24D11/0214; F24D11/0235; F24D11/025; F24V; F24V99/00; F25; F25B; F25B30/00; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/5005; G01N33/5008; G01N33/5014; G01N33/5017; Y; Y02; Y02B; Y02B30/00; Y02B30/52; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
69 words
A method for determining mutagenicity of a substance, comprising the steps of: a. exposing a culture of AHH-1 cells to the substance; b. thereafter growing the exposed AHH-1 cells for a phenotypic stabilization period; c. detecting the amount of mutant cells; and d. comparing the frequency of mutation of the exposed AHH-1 cells to the frequency of mutation of unexposed AHH-1 cells to determine the mutagenicity of the substance.
Claim 2
Independent Claim
69 words
A method for determining mutagenicity of a substance, comprising the steps of: a. exposing a culture of AHH-1 cells to the substance; b. thereafter growing the exposed AHH-1 cells for a phenotypic stabilization period; c. detecting the amount of mutant cells; and d. comparing the frequency of mutation of the exposed AHH-1 cells to the frequency of mutation of unexposed AHH-1 cells to determine the mutagenicity of the substance.
Claim 3
Dependent Claim
13 words
A method of claim 1 wherein the substance is a polynuclear aryl hydrocarbon.
Claim 4
Dependent Claim
18 words
A method of claim 1 wherein the substance is aflatoxin B 1 , benzo(α)-pyrene, 2-acetoaminofluorene or dimethyl nitrosamine.
Claim 5
Independent Claim
72 words
A method of determining the mutagenicity of a substance, comprising the steps of: a. dividing a culture of AHH-1 cells into at least two aliquots; b. exposing at least one of the aliquots to the substance; c. culturing the exposed aliquot and at least one unexposed aliquot for a phenotypic stabilization period; and d. comparing the frequency of mutation between the exposed and unexposed aliquots to determine the mutagenicity of the substance.
Claim 6
Independent Claim
72 words
A method of determining the mutagenicity of a substance, comprising the steps of: a. dividing a culture of AHH-1 cells into at least two aliquots; b. exposing at least one of the aliquots to the substance; c. culturing the exposed aliquot and at least one unexposed aliquot for a phenotypic stabilization period; and d. comparing the frequency of mutation between the exposed and unexposed aliquots to determine the mutagenicity of the substance.
Claim 7
Dependent Claim
42 words
A method of claim 4, wherein the frequency of genetic change is determined by contacting the exposed and unexposed aliquots with a selective agent during the culturing step and comparing the effect of the selective agent on the cells of each aliquot.
Claim 8
Dependent Claim
15 words
A culture consisting essentially of the human cell line AHH-1, ATCC accession number CRL 8146.
Claim 9
Dependent Claim
15 words
A culture consisting essentially of the human cell line AHH-1, ATCC accession number CRL 8146.
References Cited (Backward Citations)
US4036693A
Cited
US4066510A
Cited
US4299915A
Cited
US4302535A
Cited
WO1981003663A1
Cited
US4393133A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-07-29 | 1989-01-28 | 1989-07-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-07-29 | 1993-01-28 | 1993-07-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-07-29 | 1997-01-28 | 1997-07-30 | $7700.00 | Window Closed / Lapsed |