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US4299915A
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Assay for mutagenesis in bacterial cells
Assignee: Massachusetts Institute of Technology
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Filing: 1977-10-17
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Granted: 1981-11-10
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Expires: 1997-10-17
Abstract
Abstract An assay is disclosed for determining mutagenic damage caused by the administration of a known or suspected mutagen to bacterial cells such as Salmonella typhimurium. After administration of or exposure to a mutagenic agent, the bacterial cells are plated in the presence of a purine analog and resistance to purine analogs is used as the genetic marker. This bioassay system can be used by genetic toxicologists to determine the potential genetic hazards from the use of a variety of suspected or known mutagens, including newly-developed chemicals.
Inventors
- 👤 William G. Thilly
- 👤 Thomas R. Skopek
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 05:42
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/025
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
82 words
An assay for determining mutagenesis, comprising: a. exposing a culture of bacterial cells to an agent to be tested for its mutagenic effects on said cells and then adding an active drug-metabolizing system to said culture of bacterial cells; b. incubating exposed cells in the presence of a purine analog which acts as a purine phosphoribosyl transferase substrate and under conditions which allow said exposed cells to express phenotypically-developed resistance to said purine analog; and, c. determining the fraction of mutant cells.
Claim 2
Dependent Claim
82 words
An assay for determining mutagenesis, comprising: a. exposing a culture of bacterial cells to an agent to be tested for its mutagenic effects on said cells and then adding an active drug-metabolizing system to said culture of bacterial cells; b. incubating exposed cells in the presence of a purine analog which acts as a purine phosphoribosyl transferase substrate and under conditions which allow said exposed cells to express phenotypically-developed resistance to said purine analog; and, c. determining the fraction of mutant cells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-11-09 | 1985-05-11 | 1985-11-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-11-09 | 1989-05-11 | 1989-11-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-11-09 | 1993-05-11 | 1993-11-10 | $7700.00 | Window Closed / Lapsed |