Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
89 words
An assay for determining mutagenesis, comprising: a. exposing a culture of human diploid or near-diploid cells capable of continuous division in suspension culture and having one active x-chromosome which carries the gene for HGPRT to an agent to be tested for its mutagenic effects on said cells; b. incubating exposed cells for a number of generations sufficient to allow full expression of phenotypically-developed resistance to a purine which acts as an HGPRT substrate; and then, c. detecting the amount of mutant cells to thereby determine the degree of mutagenesis.
Claim 2
Dependent Claim
89 words
An assay for determining mutagenesis, comprising: a. exposing a culture of human diploid or near-diploid cells capable of continuous division in suspension culture and having one active x-chromosome which carries the gene for HGPRT to an agent to be tested for its mutagenic effects on said cells; b. incubating exposed cells for a number of generations sufficient to allow full expression of phenotypically-developed resistance to a purine which acts as an HGPRT substrate; and then, c. detecting the amount of mutant cells to thereby determine the degree of mutagenesis.
Claim 3
Dependent Claim
10 words
The assay of claim 1 wherein said purine is 6-thioguanine.
Claim 4
Dependent Claim
12 words
The assay of claim 1 wherein said cells comprise human diploid lymphoblasts.
Claim 5
Dependent Claim
10 words
The assay of claim 3 wherein said purine is 6-thioguanine.
Claim 6
Dependent Claim
16 words
The assay of claim 1 wherein an active, drug-metabolizing system is added to the cell cultures.
Claim 7
Dependent Claim
16 words
The assay of claim 4 wherein an active, drug-metabolizing system is added to the cell cultures.
Claim 8
Dependent Claim
33 words
The assay of claim 6 wherein said active, drug-metabolizing system comprises a post-mitochondrial supernatant of homogenized mammalian tissue in an aqueous suspension medium, said post-mitochondrial supernatant having been filtered to remove contaminating bacteria.
Claim 9
Dependent Claim
12 words
The assay of claim 7 wherein said drug-metabolizing system also contains NADH.
Claim 10
Dependent Claim
75 words
The assay for determining mutagenesis comprising: a. exposing a culture of a human, diploid, lymphoblastoid cell line to an agent to be tested under conditions whereby at least some of the cells survive; b. resuspending exposed cells in fresh medium; c. incubating the resuspended, exposed cells for a sufficient number of generations to allow the expression of the substantially fully phenotypically-developed cell resistance to a purine HGPRT substrate; and, d. determining the induced mutant fraction.
Claim 11
Dependent Claim
75 words
The assay for determining mutagenesis comprising: a. exposing a culture of a human, diploid, lymphoblastoid cell line to an agent to be tested under conditions whereby at least some of the cells survive; b. resuspending exposed cells in fresh medium; c. incubating the resuspended, exposed cells for a sufficient number of generations to allow the expression of the substantially fully phenotypically-developed cell resistance to a purine HGPRT substrate; and, d. determining the induced mutant fraction.
Claim 12
Dependent Claim
12 words
The assay of claim 9 wherein said purine HGPRT substrate is 6-thioguanine.