US4393133A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Human hepatoma derived cell line, process for preparation thereof, and uses therefor
Assignee: The Wistar Institute
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Filing: 1980-06-12
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Granted: 1983-07-12
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Expires: 2000-06-12
Abstract
Abstract Human hepatoma cell lines, useful for metabolic studies such as screening potential carcinogens and mutagens, for cultivation of viruses, and for preparation of vaccines is obtained by culturing human hepatocarcinoma or hepatoblastoma on lethally irradiated cell feeder layers in the presence of a culture medium.
Inventors
- 👤 Barbara B. Knowles
- 👤 David P. Aden
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 20:15
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/067; C12N5/0693; C12N7/00; A; A61; A61K; A61K39/00; C12N2502/00; C12N2502/13; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S436/00; Y10S436/82
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
92 words
A process for assessing the metabolic conversion of chemicals and drugs considered to be potential carcinogens and mutagens, which comprises: (a) maintaining a culture of a human hepatic cell line selected from the group consisting of the cell lines designated Hep 3B and Hep G2 in a nutrient medium, (b) exposing said cell line to the chemical or drug to be tested, (c) analyzing said culture for the presence of metabolites of said chemical or drug, and (d) introducing said metabolites to cultures of other mammalian cells to determine their mutagenic capabilities.
Claim 2
Dependent Claim
92 words
A process for assessing the metabolic conversion of chemicals and drugs considered to be potential carcinogens and mutagens, which comprises: (a) maintaining a culture of a human hepatic cell line selected from the group consisting of the cell lines designated Hep 3B and Hep G2 in a nutrient medium, (b) exposing said cell line to the chemical or drug to be tested, (c) analyzing said culture for the presence of metabolites of said chemical or drug, and (d) introducing said metabolites to cultures of other mammalian cells to determine their mutagenic capabilities.
Claim 3
Dependent Claim
14 words
The process according to claim 1 in which said cell line comprises Hep G2.
Claim 4
Dependent Claim
14 words
The process according to claim 1 in which said cell line comprises Hep 3B.
Claim 5
Dependent Claim
64 words
A process for isolation of hepatitis B virus surface antigen for use as a vaccine which comprises: (a) maintaining a human hepatic cell line, designated Hep 3B, which contains the hepatitis B virus genome in nutrient culture medium, (b) recovering the supernatant fluid from said culture, and (c) purifying the hepatitis B virus surface antigen in said supernatant fluid for use as a vaccine.
Claim 6
Dependent Claim
64 words
A process for isolation of hepatitis B virus surface antigen for use as a vaccine which comprises: (a) maintaining a human hepatic cell line, designated Hep 3B, which contains the hepatitis B virus genome in nutrient culture medium, (b) recovering the supernatant fluid from said culture, and (c) purifying the hepatitis B virus surface antigen in said supernatant fluid for use as a vaccine.
Claim 7
Dependent Claim
63 words
A process for isolation of human plasma proteins which comprises: (a) maintaining a human hepatic cell line which synthesizes plasma proteins selected from the group consisting of the cell lines designated Hep 3B and Hep G2 in a nutrient culture medium, (b) recovering the supernatant fluid from said culture, and (c) separating plasma proteins from said supernatant fluid, and purifying said plasma proteins.
Claim 8
Dependent Claim
63 words
A process for isolation of human plasma proteins which comprises: (a) maintaining a human hepatic cell line which synthesizes plasma proteins selected from the group consisting of the cell lines designated Hep 3B and Hep G2 in a nutrient culture medium, (b) recovering the supernatant fluid from said culture, and (c) separating plasma proteins from said supernatant fluid, and purifying said plasma proteins.
Claim 9
Dependent Claim
14 words
The process according to claim 5 in which said cell line comprises Hep G2.
Claim 10
Dependent Claim
14 words
The process according to claim 5 in which said cell line comprises Hep 3B.
Claim 11
Dependent Claim
10 words
The cell line having the identifying characteristics of Hep 3B.
Claim 12
Dependent Claim
10 words
The cell line having the identifying characteristics of Hep 3B.
Claim 13
Dependent Claim
10 words
The cell line having the identifying characteristics of Hep G2.
Claim 14
Dependent Claim
10 words
The cell line having the identifying characteristics of Hep G2.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-07-11 | 1987-01-10 | 1987-07-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-07-11 | 1991-01-10 | 1991-07-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-07-11 | 1995-01-10 | 1995-07-12 | $7700.00 | Window Closed / Lapsed |