US4209587A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of tumor angiogenesis factor by cell culture
Assignee: Monsanto Co
•
Filing: 1978-12-29
•
Granted: 1980-06-24
•
Expires: 1998-12-29
Abstract
Abstract Process for the production of human TAF in vitro comprising growing the human liver adenocardinoma cell line SK-HEP-1 in agitated, liquid suspension of nutrient culture medium at about 35 DEG -38 DEG C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.
Inventors
- 👤 William R. Tolbert
- 👤 Joseph Feder
- 👤 Mau-Jung Kuo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 20:05
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4748; C07K14/475; C07K14/515; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
48 words
Process for the production of human TAF in vitro comprising growing the human liver adenocarcinoma cell line SK-HEP-1 in agitated, liquid suspension of nutrient culture medium at about 35°-38° C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.
Claim 2
Dependent Claim
48 words
Process for the production of human TAF in vitro comprising growing the human liver adenocarcinoma cell line SK-HEP-1 in agitated, liquid suspension of nutrient culture medium at about 35°-38° C. for a sufficient time to elaborate TAF and recovering the resulting TAF from the cells or cell product.
Claim 3
Dependent Claim
19 words
The process of claim 1 in which the nutrient culture medium in Dulbecco's modification of Eagle's minimum essential medium.
Claim 4
Dependent Claim
16 words
The process of claim 1 in which the nutrient culture medium is fortified with mammalian serum.
Claim 5
Dependent Claim
23 words
The process of claim 1 in which the nutrient culture medium is fortified with from about 10% to about 15% fetal bovine serum.
Claim 6
Dependent Claim
16 words
The process of claim 1 in which the TAF is recovered by extraction from the cells.
Claim 7
Dependent Claim
38 words
The process of claim 1 in which the nutrient culture medium is Dulbecco's modification of Eagle's minimum essential medium and is fortified with fetal bovine serum and in which the TAF is recovered by extraction from the cells.
References Cited (Backward Citations)
US4059486A
Cited
US4059485A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-06-24 | 1983-12-24 | 1984-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-06-24 | 1987-12-24 | 1988-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-06-24 | 1991-12-24 | 1992-06-24 | $7700.00 | Window Closed / Lapsed |