US4059486A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture process
Assignee: Monsanto Co
•
Filing: 1976-11-03
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Granted: 1977-11-22
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Expires: 1996-11-03
Abstract
Abstract SV3T3 cells are rapidly propagated in vitro to elaborate MIF and TAF by first growing on a surface and thereafter transferring an inoculum of the growing cells into an agitated liquid suspension of nutrient culture medium, incubating at 30 DEG -38 DEG C while continuing the agitation of the medium, periodically transferring the growing cells into fresh medium and continuing the incubation with agitation of the medium, whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
Inventors
- 👤 William R. Tolbert
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 23:03
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; A; A61; A61P; A61P43/00; C07K14/475; C07K14/515
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
101 words
A method of rapidly propagating SV3T3 cells in vitro to elaborate MIF and TAF comprising growing SV3T3 cells on a surface, thereafter transferring an inoculum of said growing cells into a normally liquid suspension of nutrient culture medium of known essential amino acids, mineral salts, vitamins, and carbohydrates, incubating at a temperature of from about 30° to about 38° C while substantially continuously maintaining agitation of said medium, periodically transferring the growing cells into fresh nutrient culture medium and continuing the agitated incubation until harvest whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
Claim 2
Independent Claim
101 words
A method of rapidly propagating SV3T3 cells in vitro to elaborate MIF and TAF comprising growing SV3T3 cells on a surface, thereafter transferring an inoculum of said growing cells into a normally liquid suspension of nutrient culture medium of known essential amino acids, mineral salts, vitamins, and carbohydrates, incubating at a temperature of from about 30° to about 38° C while substantially continuously maintaining agitation of said medium, periodically transferring the growing cells into fresh nutrient culture medium and continuing the agitated incubation until harvest whereby said cells are proliferated in aggregates and elaborate MIF and TAF in significantly detectable amounts.
Claim 3
Dependent Claim
17 words
The method of claim 1 in which the nutrient culture medium is Dulbecco's Modified Minimum Essential medium.
Claim 4
Dependent Claim
18 words
The method of claim 1 in which the incubation temperature is from about 35° to about 37° C.
Claim 5
Dependent Claim
24 words
The method of claim 1 in which two periodic transfers of the growing cells into fresh nutrient culture medium are made at predetermined intervals.
Claim 6
Dependent Claim
16 words
The method of claim 1 in which the nutrient culture medium is fortified with mammalian serum.
Claim 7
Dependent Claim
14 words
The method of claim 5 in which the mammalian serum is fetal calf serum.
Claim 8
Dependent Claim
14 words
The method of claim 1 in which the SV3T3 cells are BALB/c SV3T3 cells.
Claim 9
Dependent Claim
58 words
The method of claim 1 in which the SV3T3 cells are BALB/c SV3T3 cells, the nutrient culture medium is Dulbecco's Modified Minimum Essential medium fortified with fetal calf serum, the incubation temperature is from about 35° to about 37° C and two periodic transfers of the growing cells into fresh nutrient culture medium are made at predetermined intervals.
Claim 10
Dependent Claim
21 words
The method of claim 1 including the additional step of harvesting the cells and extracting therefrom an active concentrate of MIF.
Claim 11
Dependent Claim
21 words
The method of claim 1 including the additional step of harvesting the cells and extracting therefrom an active concentrate of TAF.
References Cited (Backward Citations)
US3850748A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1980-11-21 | 1981-05-23 | 1981-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1984-11-21 | 1985-05-23 | 1985-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1988-11-21 | 1989-05-23 | 1989-11-22 | $7700.00 | Window Closed / Lapsed |