Patent US4089122A successfully refreshed from Google Patents.
Patent US4523997A successfully refreshed from Google Patents.
US4523997A
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Affinity chromatography matrix with built-in reaction indicator
Assignee: Avantor Performance Materials LLC
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Filing: 1984-03-02
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Granted: 1985-06-18
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Expires: 2004-03-02
Abstract
Abstract p-Nitrophenylester of succinoylaminopropyl silica gel is useful as an affinity matrix for reacting with ligand for use in affinity chromatography. The affinity matrix, upon reaction with a ligand, releases one molecule of p-nitrophenolate ion for every molecule of ligand that reacts and the solution turns yellow as the reaction occurs.
Inventors
- 👤 Laura J. Crane
- 👤 Hugh E. Ramsden
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:59
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/291; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J20/02; B01J20/10; B01J20/103; B01J20/28; B01J20/28002; B01J20/28004; B01J20/28054; B01J20/28078; B01J20/30; B01J20/3071; B01J20/32; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/552; B01J2220/00; B01J2220/50; B01J2220/54
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
6 words
The p-nitrophenylester of succinoylaminopropyl silica gel.
Claim 2
Dependent Claim
6 words
The p-nitrophenylester of succinoylaminopropyl silica gel.
Claim 3
Dependent Claim
36 words
The product of claim 1 wherein the silica gel has an average particle diameter of from about 3 to about 70 microns and an average pore size of from about 50 to about 100 Angstrom units.
Claim 4
Dependent Claim
52 words
In a method of separating or purifying a substance from a solution by reacting the substance in the solution with an affinity matrix having a ligand covalently bound to the affinity matrix, the improvement comprising employing the p-nitrophenylester of succinoylaminopropyl silica gel having a ligand covalently bound thereto as the affinity matrix.
Claim 5
Dependent Claim
52 words
In a method of separating or purifying a substance from a solution by reacting the substance in the solution with an affinity matrix having a ligand covalently bound to the affinity matrix, the improvement comprising employing the p-nitrophenylester of succinoylaminopropyl silica gel having a ligand covalently bound thereto as the affinity matrix.
Claim 6
Dependent Claim
24 words
The method of claim 3 wherein the silica gel affinity matrix has an average particle diameter of from about 3 to about 70 microns.
Claim 7
Dependent Claim
20 words
The method of claim 3 wherein the ligand is a protein containing an amine group reactive with the affinity matrix.
Claim 8
Dependent Claim
20 words
The method of claim 4 wherein the ligand is a protein containing an amine group reactive with the affinity matrix.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-06-17 | 1988-12-17 | 1989-06-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-06-17 | 1992-12-17 | 1993-06-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-06-17 | 1996-12-17 | 1997-06-18 | $7700.00 | Window Closed / Lapsed |