US4212905A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of coating supports using addition copolymers of aminimides
Assignee: University of Florida
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Filing: 1978-06-20
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Granted: 1980-07-15
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Expires: 1998-06-20
Abstract
Abstract A polymer suitable for use in affinity chromatography comprising a polymerf an aminimide and a vinyl compound having at least one pendant halo-methyl group and the said polymer wherein an amine ligand which affords sites for binding in affinity chromatography is coupled to the polymer by reaction with a portion of the pendant halo-methyl groups and the remainder of said pendant halo-methyl groups are reacted with an amine containing a pendant hydrophilic group. The invention also comprises a method of coating a substrate with the above polymer and a method of fractionating mixtures by affinity chromatography employing the above polymer as an adsorbent.
Inventors
- 👤 John C. M. Tsibris
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 05:52
CPC Classifications
B; B01; B01J; B01J20/00; B01J20/281; B01J20/282; B01J20/285; B01J20/22; B01J20/26; B01J20/261; B01J20/264; B01J20/265; B01J20/286; B01J20/30; B01J20/3092; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3214; B01J20/3217; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/3276; C; C07; C07J; C07J41/00; C07J41/0005; C08; C08F; C08F8/00; C08F8/30; C08F8/32; B01J2220/00; B01J2220/50; B01J2220/54; B01J2220/58; Y; Y10; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2991; Y10T428/2993; Y10T428/2996; Y10T428/31504; Y10T428/3154; Y10T428/31544; Y10T428/31678; Y10T428/31681
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
148 words
A method of coating a support with an addition copolymer of (1) at least one aminimide of the formula: ##STR8## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group; and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant primary halomethyl group, said polymer having coupled thereto by reaction with a portion of the pendant primary halomethyl groups an amine ligand which affords sites for binding in affinity chromatography, comprising contacting a support with a solvent solution of the polymer, removing said solvent and contacting said coated support with a non-solvent for the final polymer containing a primary amine having a pendant hydrophilic group, whereby said amine reacts with the remainder of said primary halomethyl groups and enhances the bond between said polymer and said support.
Claim 2
Independent Claim
148 words
A method of coating a support with an addition copolymer of (1) at least one aminimide of the formula: ##STR8## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group; and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant primary halomethyl group, said polymer having coupled thereto by reaction with a portion of the pendant primary halomethyl groups an amine ligand which affords sites for binding in affinity chromatography, comprising contacting a support with a solvent solution of the polymer, removing said solvent and contacting said coated support with a non-solvent for the final polymer containing a primary amine having a pendant hydrophilic group, whereby said amine reacts with the remainder of said primary halomethyl groups and enhances the bond between said polymer and said support.
Claim 3
Dependent Claim
14 words
A method according to claim 1 wherein said non-solvent for said polymer contains monoethanolamine.
Claim 4
Dependent Claim
12 words
A method according to claim 1 wherein said support comprises glass beads.
Claim 5
Dependent Claim
25 words
A method according to claim 1 wherein said aminimide is 1,1-dimethyl-1-(2-hydroxyoctyl)amine methacrylimide, said vinyl compound is a chloromethylstyrene and said primary amine ligand is ##STR9##
Claim 6
Dependent Claim
25 words
A method according to claim 1 wherein said aminimide is 1,1-dimethyl-1-(2-hydroxyoctyl)amine methacrylimide, said vinyl compound is a chloromethylstyrene and said primary amine ligand is: ##STR10##
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-15 | 1984-01-14 | 1984-07-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-15 | 1988-01-14 | 1988-07-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-15 | 1992-01-14 | 1992-07-15 | $7700.00 | Window Closed / Lapsed |