US4213860A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Affinity chromatography and substrate useful therefor
Assignee: University of Florida
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Filing: 1978-06-29
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Granted: 1980-07-22
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Expires: 1998-06-29
Abstract
Abstract A polymer suitable for use in affinity chromatography comprising a polymer of an aminimide and a vinyl compound having at least one pendant halo-methyl group and the said polymer wherein an amine ligand which affords sites for binding in affinity chromatography is coupled to the polymer by reaction with a portion of the pendant halo-methyl groups and the remainder of said pendant halo-methyl groups are reacted with an amine containing a pendant hydrophilic group.
Inventors
- 👤 John C. M. Tsibris
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 03:40
CPC Classifications
C; C07; C07J; C07J41/00; C07J41/0005; B; B01; B01D; B01D15/00; B01D15/08; B01D15/26; B01D15/38; B01D15/3804; B01J; B01J20/00; B01J20/22; B01J20/26; B01J20/261; B01J20/281; B01J20/286; B01J20/288; B01J20/30; B01J20/32; B01J20/3202; B01J20/3204; B01J20/3231; B01J20/3242; B01J20/3268; B01J20/327; B01J20/3276; B01J20/3291; B01J20/3293; C08; C08F; C08F8/00; C08F8/30; C08F8/32; B01J2220/00; B01J2220/50; B01J2220/58
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
117 words
A system suitable for affinity chromatography comprising a column of support material coated with an addition copolymer of (1) at least one aminimide of the structural formula: ##STR8## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups, the remainder of said pendant halomethyl groups being reacted with an amine containing a pendant hydrophilic group.
Claim 2
Independent Claim
117 words
A system suitable for affinity chromatography comprising a column of support material coated with an addition copolymer of (1) at least one aminimide of the structural formula: ##STR8## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups, the remainder of said pendant halomethyl groups being reacted with an amine containing a pendant hydrophilic group.
Claim 3
Dependent Claim
12 words
The system of claim 1 wherein said support material comprises glass beads.
Claim 4
Independent Claim
113 words
A system suitable for affinity chromatography comprising a column of an addition copolymer of (1) at least one aminimide of the structural formula: ##STR9## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups, the remainder of said pendant halomethyl groups being reacted with an amine containing a pendant hydrophilic group.
Claim 5
Independent Claim
113 words
A system suitable for affinity chromatography comprising a column of an addition copolymer of (1) at least one aminimide of the structural formula: ##STR9## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups, the remainder of said pendant halomethyl groups being reacted with an amine containing a pendant hydrophilic group.
Claim 6
Independent Claim
101 words
A system suitable for affinity chromatography comprising a column of support material coated with an addition copolymer of (1) at least one aminimide of the structural formula: ##STR10## wherein R 1 and R 2 are the ame or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said addition copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups.
Claim 7
Independent Claim
101 words
A system suitable for affinity chromatography comprising a column of support material coated with an addition copolymer of (1) at least one aminimide of the structural formula: ##STR10## wherein R 1 and R 2 are the ame or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said addition copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups.
Claim 8
Independent Claim
98 words
A system suitable for affinity chromatography comprising a column of an addition of copolymer of (1) at least one aminimide of the structural formula: ##STR11## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said addition copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups.
Claim 9
Independent Claim
98 words
A system suitable for affinity chromatography comprising a column of an addition of copolymer of (1) at least one aminimide of the structural formula: ##STR11## wherein R 1 and R 2 are the same or different and represent lower alkyl; R 3 represents an organic group, and Z represents the residue of a polymerizable vinyl compound, and (2) a vinyl compound having at least one pendant halomethyl group; said addition copolymer having coupled thereto an amine ligand which affords sites for binding an affinity chromatography by reaction of said amine with a portion of the pendant halomethyl groups.
Claim 10
Dependent Claim
45 words
In an affinity chromatography method wherein an absorbent having an affinity for at least one material is contacted with a liquid mixture containing said at least one material to separate said at least one material therefrom, the improvement comprising employing the absorbent of claim 1.
Claim 11
Dependent Claim
45 words
In an affinity chromatography method wherein an absorbent having an affinity for at least one material is contacted with a liquid mixture containing said at least one material to separate said at least one material therefrom, the improvement comprising employing the absorbent of claim 3.
Claim 12
Dependent Claim
44 words
In an affinity chromatography method wherein an absorbent having an affinity for at least one material is contacted with a liquid containing said at least one material to separate said at least one material therefrom, the improvement comprising employing the absorbent of claim 4.
Claim 13
Dependent Claim
45 words
In an affinity chromatography method wherein an absorbent having an affinity for at least one material is contacted with a liquid mixture containing said at least one material to separate said at least one material therefrom, the improvement comprising employing the absorbent of claim 5.
Claim 14
Dependent Claim
16 words
The method of claim 6 wherein said chromatography method is a high pressure liquid chromatography method.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-07-22 | 1984-01-21 | 1984-07-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-07-22 | 1988-01-21 | 1988-07-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-07-22 | 1992-01-21 | 1992-07-22 | $7700.00 | Window Closed / Lapsed |