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US4478935A
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Manganese-containing antibiotic agents
Assignee: International Minerals and Chemical Corp
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Filing: 1982-09-20
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Granted: 1984-10-23
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Expires: 2002-09-20
Abstract
Abstract Manganese complexes of various monovalent and divalent polyether antibiotics are provided which act as coccidiostats and growth promoting agents when administered to food-producing animals such as cattle, sheep, swine and poultry. Soluble manganese salt is added to a fermentation beer containing the polyether antibiotics to form an insoluble, recoverable biomass containing the desired manganese antibiotic complex. This biomass, in a dried form, can be given orally to animals such as cattle, sheep, swine and poultry. The subject manganese complexes include: linear monovalent and divalent polyethers (monensin, nigericin, lasalocid, lysocellin, etc.); non-glycolic monovalent monoglycoside polyethers (septamycin, dianemycin, lenoremycin, carriomycin and antibiotic A-204); mononitrogen-containing divalent pyrrole ethers (calcimycin, X-14547, etc.); polynitrogen-containing divalent pyrrole ethers (A-23187, etc.); glycolic monovalent monoglycoside polyethers (etheromycin, etc.); other polyether antibiotics including ionomycin, aabomycin, disnerycin, duamycin, BL-580, K-41, SF-1195, M-4164A, A-32887, 30,504RP, 38,986, 44,161, 47,433, 47,434 and 47,224. The above manganese complexes are also active agents for improving cardiovascular function in animals. Purified manganese-containing antibiotic complexes can be extracted from the biomass using suitable organic solvents, followed by crystallization or precipitation of the purified complexes. The purified products can be administered as boluses, subcutaneous implants, or as injectable preparations.
Inventors
- 👤 Robert D. Williams
- 👤 David R. Bright
- 👤 Vernon V. Young
- 👤 Jerome L. Martin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 14:24
CPC Classifications
C; C07; C07H; C07H23/00; C12; C12N; C12N1/00; C12N1/20; C12N1/205; C12P; C12P17/00; C12P17/16; C12P17/162; C12P19/00; C12P19/44; C12P19/445; C12R; C12R2001/00; C12R2001/01; C12R2001/465
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
90 words
A method for producing a manganese complex of a polyether antibiotic, said method comprising: (a) fermenting a nutrient broth inoculated with a Streptomyces microorganism capable of producing by growth in the broth a polyether antibiotic for a period of time and under suitable fermentation conditions in order to produce said polyether antibiotic in said fermentation broth; and (b) providing in said antibiotic containing fermented broth a water-soluble manganese salt in an amount sufficient to form a manganese complex of said polyether antibiotic, which complex is insoluble in the fermented broth.
Claim 2
Independent Claim
90 words
A method for producing a manganese complex of a polyether antibiotic, said method comprising: (a) fermenting a nutrient broth inoculated with a Streptomyces microorganism capable of producing by growth in the broth a polyether antibiotic for a period of time and under suitable fermentation conditions in order to produce said polyether antibiotic in said fermentation broth; and (b) providing in said antibiotic containing fermented broth a water-soluble manganese salt in an amount sufficient to form a manganese complex of said polyether antibiotic, which complex is insoluble in the fermented broth.
Claim 3
Dependent Claim
19 words
The method of claim 1 further including the step of recovering said insoluble manganese complex from said fermented broth.
Claim 4
Dependent Claim
35 words
The method of claim 1 wherein the fermentation of the inoculated broth is conducted at a temperature of from about 25° C. to 35° C. and at a pH of from about 6.5 to 7.5.
Claim 5
Dependent Claim
28 words
The method in accordance with claim 2 or 3 wherein the manganese salt is added to the fermented broth and is selected from manganese chloride and manganese sulfate.
Claim 6
Dependent Claim
24 words
The method of claim 2 or 3 wherein the Streptomyces microorganism employed is Streptomyces lasaliensis and the polyether antibiotic complex produced is manganese lasalocid.
Claim 7
Dependent Claim
24 words
The method of claim 2 or 3 wherein the Streptomyces microorganism employed is Streptomyces cinnamonensis and the polyether antibiotic complex produced is manganese monensin.
Claim 8
Dependent Claim
24 words
The method of claim 2 or 3 wherein the Streptomyces microorganism employed is Streptomyces longwoodensis and the polyether antibiotic complex produced is manganese lysocellin.
Claim 9
Dependent Claim
90 words
The method of claim 2 or 3 wherein the Streptomyces microorganism employed is selected from the following list to produce the manganese polyether antibiotic specified below: ______________________________________
S. sp. X14547 Manganese X-14547
S. chartreusis Manganese A-23187
S. hygroscopicus sp.
Manganese etheromycin
S. hygroscopicus
Manganese carriomycin
S. albus sp. Manganese salinomycin
S. albus sp. Manganese alborixin
S. albus sp. Manganese SY-1
S. aureofaciens Manganese narasin
S. noboritoensis
Manganese noboritomycin A & B
S. griseus Manganese grisorixin
S. ribosidicus Manganese lonomycin
S. mutabilis Manganese mutalomycin
S. eurocidicus var.
Manganese laidlomycin
asterocidicus
______________________________________
Claim 10
Dependent Claim
39 words
A method in accordance with claim 2 wherein the insoluble manganese antibiotic complex is recovered from said fermented broth as part of a biomass which additionally contains insoluble manganese complexes of residual nitrogen-containing compounds present in the fermentation broth.
Claim 11
Dependent Claim
48 words
A method in accordance with claim 9 wherein water soluble manganese salt is added to the fermented broth in an amount sufficient to provide a manganese content of from about 3 to 12 percent by weight on a dry basis in the biomass recovered from said fermentation broth.
Claim 12
Dependent Claim
94 words
A method in accordance with claim 1 further including the steps of: (c) acidifying the fermented broth containing the insoluble manganese complex to form the acidic form of the polyether antibiotic; (d) extracting the polyether antibiotic from the broth into a water-insoluble organic solvent; (e) adding a lower aliphatic alcohol to the organic solvent containing the polyether antibiotic, the alcohol containing a soluble manganese salt in an amount sufficient to form a manganese complex of the polyether antibiotic; and (f) recovering the formed manganese complex of the polyether antibiotic from the organic solvent-alcohol mixture.
Claim 13
Dependent Claim
13 words
A method in accordance with claim 11 wherein the aliphatic alcohol is methanol.
Claim 14
Independent Claim
16 words
A method in accordance with claims 11 or 18 wherein the organic solvent is butyl acetate.
Claim 15
Dependent Claim
20 words
A method in accordance with claim 11 wherein the fermentation broth is acidified to a pH of 4.0 or less.
Claim 16
Dependent Claim
18 words
A method in accordance with claim 11 wherein the manganese salt used in step (e) is manganese chloride.
Claim 17
Dependent Claim
28 words
A method in accordance with claim 11 further including the step of adding an aliphatic alcohol to the organic solvent containing the polyether antibiotic prior to step (e).
Claim 18
Dependent Claim
42 words
A method in accordance with claim 11 further including the steps of extracting the polyether antibiotic from the organic solvent into an aqueous alkaline solution and then extracting the polyether antibiotic from the aqueous alkaline solution into a second water-insoluble organic solvent.
Claim 19
Dependent Claim
27 words
A method in accordance with claim 17 wherein the aqueous alkaline solution contains a metal hydroxide selected from the group consisting of sodium hydroxide and potassium hydroxide.
Claim 20
Dependent Claim
26 words
A method in accordance with claim 17 wherein the second water-insoluble organic solvent is selected from the group consisting of butyl acetate and methyl tertiary-butyl ether.
References Cited (Backward Citations)
US3923823A
Cited
US3873715A
Cited
US4083968A
Cited
US4164586A
Cited
US4058620A
Cited
US4076834A
Cited
US3985893A
Cited
US3907832A
Cited
US4129578A
Cited
US4100171A
Cited
US4181573A
Cited
US4161520A
Cited
US4148890A
Cited
US4129659A
Cited
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