US4474878A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Sandwich EIA for antigen associated with hepatitis
Assignee: Diamedix Corp
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Filing: 1975-09-29
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Granted: 1984-10-02
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Expires: 1995-09-29
Abstract
Abstract A sensitive direct immunoassay system is provided for the detection of an antigen associated with hepatitis in body fluids. A single antibody which reacts with a hepatitis antigen or antigens and which is bonded to an insoluble member, is incubated with a test sample. During this first period of incubation a portion of an antigen present in the test sample will combine with the antibody immobilized on the insoluble member. The antibody bonded member, to which antigen is attached, is then washed and incubated with an enzyme tagged antibody reagent. During the second incubation, the tagged antibody reacts with antigen fixed to the antibody member in the first incubation. Thus, an immobilized "sandwich" is formed of an insoluble member- antibody-antigen-enzyme tagged antibody. After the second incubation, the member is washed again to remove unreacted enzyme antibody reagent. The member is then exposed to a substrated which is converted by the enzyme to produce an end product. The tagged antibody reagent will be fixed in the second incubation only if antigen was present in the sample. The amount of enzyme tagged antibody fixed is proportional to the amount of antigen of antigens present in the test sample up to the maximum capacity of the test. The concentration of the end product, and hence the amount of antigen or antigens, is determined by a spectrophotometer which measures the optical absorption of light by the end product. This readout is then compared against a standard value for both antigen negative and antigen positive samples.
Inventors
- 👤 Seymour P. Halbert
- 👤 Milton Anken
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 18:52
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N33/576; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S436/00; Y10S436/811; Y10S436/82
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
227 words
A process for determining the presence of antigens associated with hepatitis in a sample comprising the following steps: (a) incubating the sample with an antibody immobilized on an insoluble member, the antibody being reactive with the antigens associated with hepatitis, the incubation enabling a bond to be formed between the antibody and an antigen present in the sample to produce an insoluble member having an antigen bonded thereto in the event antigens associated with hepatitis are present in the sample; (b) separating the insoluble member from any unbonded substances; (c) incubating the insoluble member with a solution containing tagged antibody reactive with an antigen associated with hepatitis, the tagged antibody being tagged with an enzyme capable of effecting a reaction of a substrate to produce a detectable reaction product, the incubation being conducted to enable the tagged antibody to bond to any antigen bonded in step (a) to the antibody on the insoluble member; (d) separating the insoluble member from the enzyme tagged antibody solution to remove any unbonded tagged antibody therefrom; (e) exposing the insoluble member to a substrate solution which the enzyme of the tagged antibody reacts upon to enable a chemical change in the substrate to take place and to produce a detectable reaction product; and, (f) detecting any reaction product present in the solution to determine the presence of antigens associated with hepatitis.
Claim 2
Dependent Claim
227 words
A process for determining the presence of antigens associated with hepatitis in a sample comprising the following steps: (a) incubating the sample with an antibody immobilized on an insoluble member, the antibody being reactive with the antigens associated with hepatitis, the incubation enabling a bond to be formed between the antibody and an antigen present in the sample to produce an insoluble member having an antigen bonded thereto in the event antigens associated with hepatitis are present in the sample; (b) separating the insoluble member from any unbonded substances; (c) incubating the insoluble member with a solution containing tagged antibody reactive with an antigen associated with hepatitis, the tagged antibody being tagged with an enzyme capable of effecting a reaction of a substrate to produce a detectable reaction product, the incubation being conducted to enable the tagged antibody to bond to any antigen bonded in step (a) to the antibody on the insoluble member; (d) separating the insoluble member from the enzyme tagged antibody solution to remove any unbonded tagged antibody therefrom; (e) exposing the insoluble member to a substrate solution which the enzyme of the tagged antibody reacts upon to enable a chemical change in the substrate to take place and to produce a detectable reaction product; and, (f) detecting any reaction product present in the solution to determine the presence of antigens associated with hepatitis.
Claim 3
Dependent Claim
23 words
The process as set forth in claim 1 wherein the reaction product is detected by measuring the optical absorption of the substrate solution.
Claim 4
Dependent Claim
14 words
The process as set forth in claim 1 wherein the enzyme is alkaline phosphatase.
Claim 5
Dependent Claim
14 words
The process as set forth in claim 3 wherein the substrate is p-nitrophenyl phosphate.
Claim 6
Dependent Claim
33 words
The process as set forth in claim 3 wherein the substrate is selected from the group consisting of β-glycerol phosphate, phenyl phosphate, β-naphthyl phosphate, p-nitrophenyl phosphate, phenolphthalein phosphate, thymolphthalein phosphate, and 4-methylumbelliferyl phosphate.
Claim 7
Dependent Claim
66 words
The process as set forth in claim 1 wherein the enzyme is selected from the group consisting of alcohol dehydrogenase, glycerol dehydrogenase, glyoxylate reductase, L-lactate dehydrogenase, malate dehydrogenase, glucose 6-phosphate dehydrogenase, mannitol 1-phosphate dehydrogenase, glucose oxidase, galactose oxidase, L-amino acid oxidase, D-amino acid oxidase, polyphenol oxidase, ascorbate oxidase, catalase, peroxidase, cholinesterase, a-amylase, lysozyme, β-galactosidase, amyloglucosidase, β-glucuronidase, carboxypeptidase A, urease, inorganic pyrophosphatase, aldolase, carbonic anhydrase, and histidase.
Claim 8
Dependent Claim
33 words
The process as set forth in claim 1 wherein the value obtained in detecting any reaction product is compared with the value obtained from a sample known to contain antigens associated with hepatitis.
Claim 9
Dependent Claim
31 words
The process as set forth in claim 1 wherein the insoluble member is a polymeric member having an antibody reactive with an antigen associated with hepatitis covalently bonded to its surface.
Claim 10
Dependent Claim
19 words
The process as set forth in claim 1 wherein the sample incubated in step (a) is a body fluid.
Claim 11
Dependent Claim
32 words
The process as set forth in claim 9 wherein the body fluid is selected from the group consisting of serum, plasma, components of plasma, components of serum, urine, saliva, and cerebrospinal fluid.
Claim 12
Dependent Claim
21 words
The process as set forth in claim 1 wherein the antigen that is determined is the antigen associated with hepatitis B.
Claim 13
Dependent Claim
21 words
A process as set forth in claim 1 wherein a horse globulin solution is included in the incubation of step (a).
Claim 14
Independent Claim
132 words
A method for the detection of an hepatitis antigen in a liquid sample containing said antigen, comprising the steps of: a. providing a quantity of the antibody associated with said antigen, said antibody being bound to a water-insoluble, water-insuspensible, solid carrier; b. contacting and incubating said liquid sample with said solid carrier of step (a) to form a reaction mixture; c. providing a solution of a second quantity of said antibody, said second quantity of said antibody being covalently linked to an enzyme; d. contacting and incubating the solid carrier resulting from (b) with said solution of enzyme-linked antibody of (c); separating the solid carrier from the solution of enzyme-linked antibody; e. correlating the enzyme activity of substance bound to the solid phase to the presence of hepatitis antigen to be detected.
Claim 15
Independent Claim
132 words
A method for the detection of an hepatitis antigen in a liquid sample containing said antigen, comprising the steps of: a. providing a quantity of the antibody associated with said antigen, said antibody being bound to a water-insoluble, water-insuspensible, solid carrier; b. contacting and incubating said liquid sample with said solid carrier of step (a) to form a reaction mixture; c. providing a solution of a second quantity of said antibody, said second quantity of said antibody being covalently linked to an enzyme; d. contacting and incubating the solid carrier resulting from (b) with said solution of enzyme-linked antibody of (c); separating the solid carrier from the solution of enzyme-linked antibody; e. correlating the enzyme activity of substance bound to the solid phase to the presence of hepatitis antigen to be detected.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-02 | 1988-04-02 | 1988-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-02 | 1992-04-02 | 1992-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-02 | 1996-04-02 | 1996-10-02 | $7700.00 | Window Closed / Lapsed |